Contents of wells were pipetted up and down several times to mix. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Deep well blocks available in 24, 96 and 384-well formats Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. validation, maintenance, and repair documentation. . The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. White 96-well plate The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. robotics-automation-compatible. Both COC microplates, the µCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. In . 2 OD at 200 nm; this decreased to 0. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. GxP regulated industry assessments of microplate readers. Plate readers, also known as microplate readers or microplate photometers,. 50 racks/case for 384-well (16 channel pipettor head. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). After 24 hours, cells were trypsinized and seeded at 100 μL/well into a 96-well plate starting at 30,000 cells/well with subsequent 2-fold dilutions. i3x. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate:The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. Compact design Minimum volume and. 0 OD at. Suggested plates are black walled. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. Wavelength Range. Eight filter modes enable applications such as protein quantification, cell viability and ELISA. 384 Awareness Technology ChroMate BioTek Synergy Mx. 0. Small but mighty absorbance plate reader •ffordable monochromator-based plate reader with linearity A of up to 3 OD • orks with 96- and 384-well plates as well as cuvettes W (ABS Plus) • Option to run samples as low as 2 μL with the SpectraDrop™ Micro-Volume Microplate • Runs various assays within the Vis/UV-Vis range such asI tried Nunc Edge 2. 7. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Fluorescent DNA quantitation. Equipped with scanning, shaking and kinetics modules. Samples were run in triplicate. 05. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. Standard SBS 96 and 384 well plates (128. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. Our SpectraTest® ABS2, FL1, LM1,The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Microplate Formats: 6, 12, 24, 48, 96, 384. I contacted Molecular Devices and they quoted me almost $6k to come out and replace. This unlocks the transport slide. of 0. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Temperature Control: Ambient +5C up to 42C. *Simple analysis and optimization. 1 OD at 230 nm. Cross-talk < 0. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. “The ABS Plus reader comes standard with our patented PathCheck® Technology which enables consistent results across a 96- or 384-well plate. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. The EMax® Plus Microplate Reader is designed to be a versatile and robust microplate reader for research laboratories. 96-well: 0. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. field-upgradeable option with one-of-a-kind brightfield cell segmentation, green and red. It is a UV/Vis spectrophotometer/microwell reader with built-in cuvette port and microplate drawer. 2 mm x 86 mm) Height:. ValitaTiter Plus 96-well (1 plate) VAL004. tecHnical SPecificationS Performance Specifications Measurement range: 400–750 nm 0–4. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. 63. 4 Pages. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. Filters: Excitation and emission slides for 4 filters each. Temperature control. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. 2 2600A ValitaTiter. SpectraMax® 340PC384 Microplate Reader for. SmartReader 96: Designed for optimal ELISA Testing. Learn more about how absorbance is measured, and some key applications that utilize. #3676). This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. SpectraMax 250 User. Make sure to Data 96孔微孔板的数据对比. In addition, SoftMax Pro Software Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. Keywords: fluorescence polarization, trf, fret, igg. Molecular Devices is a leader in comprehensive compliance solutions with microplate detection systems. Minneapolis, MN, USA), according to the manufacturer's protocol. The Multiskan Sky microplate reader is an absorbance plate reader. Cells were washed three times with calcium wash buffer using an AquaMax 4000 Microplate Washer with 96- or 384-well cell wash head. An industry-renowned instrument for monitoring GPCRs and ion channels. 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl Recommended ArticlesA method is described for the routine determination of the rate of colorimetric enzyme reactions using a 96-well microtiter plate reader commonly used in. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. 00. Related Products: Low Binding Plates. Plate Formats: 6 to 384-well microplates. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. 001 OD Read modes (EMax): Endpoint Read modes (VMax): Endpoint, kinetic Read times (96-well microplate): Endpoint read-only: 5 seconds. 2 Pages. 1536-well: 0. Consult Molecular Devices's entire Threshold Immunoassay System catalogue on MedicalExpo. Page: 1/4. Reactions catalyzed by firefly and *Renilla luciferases. Figure 2-23: Bottle holder To install the bottle holder: 1. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. This reader also makes use of a unique temperature-independent methodology for path length correction. reagent basin for ease of pipetting into. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Both readers were heated to 37ºC prior to setting up . #PLUS 384) Methods. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 100 μL/well Steady-Luc working solution was added to the wells. 3 OD at 200 nm. For more sample throughput, both readers can be easily integrated into full roboticPrimary considerations when choosing a microplate reader are throughput and flexibility. July 17, 2023. Danaher Inc 96 well microplate reader 96 Well Microplate Reader, supplied by Danaher Inc, used in various techniques. SoftMax Pro Software allows you to set up and run a complete protocol for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. The SpectraMax® ABS Plus Microplate Reader can run . The absorbance of the tested solutions was measured at 405 nm using a SpectraMax® ABS PLUS Absorbance Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). fluorescence microplate reader from Molecular Devices. Zhu P, Ju C, Sheng J, Du D, Wan J, Yin H, Xing Y, Li H, et al. 2600A October 2023. High. The concentration of total free thiols in the protein. explore more. Operates as a stand-alone reader or with input/output stackers and bottom reading optics. Molecular Devices VersaMax Microplate Reader Specifications. Abstract and Figures. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Can read any standard cuvette, micro cuvette, test tube and 96-well or UV-transparent microplate in the wavelength range of 190-1000 nm. Description. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. 96-well microplate at 200 µL/well. #ID3-STD) L-Tryptophan (Sigma cat. Molecular Devices' microplate readers. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a black, clearbottom microplate. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. For more sample throughput, the system can be. 05. No additional manuals are available. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. 04 sec. molecular devices gmbh tel. A control well was set up to verify. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 40266. Microscopy. In addition, the iD5 reader measures TRF and FP and. Microplate luminescent reader assay for ERK activity. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. MultiWash+ Microplate Washer, A 96- & 384-well Automated Washer| Molecular Devices Author: Molecular Devices, LLCA polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. 1 pM 1536-well: 0. $489. In addition, SoftMax Pro Software In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. 3. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. U-bottom: 30-40 µl. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the. The direct transfer capability reduces reagent consumption and allows for more assay conditions to be explored in a single microplate, making the system more amenable to agonist and antagonist assay formats. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. Microplate readers can be either single-function instruments or “multimode. 0 96 Well Plate. Growth medium was then replaced with fatty acid uptake solution. LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent:. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. When the user applies a set temperature of 37°C, the top is heated to 37. book Page 9 Wednesday, July 7, 2010 2:09 PM. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofMulti-mode microplate readers with fluorescence detection. #L6876) Guanidine hydrochloride (Sigma cat. While they play an important role in generating data from a wide range of. The device was able to achieve 3σ limits of detection of ca. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. For biochemical. 2 W x 44. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. A 1:10 serial dilution of ATP was prepared in medium. 96-well microplate; Immunoassay analyzer;VersaMax price. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. 1/17 1907B Printed in USA . The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. 984. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) • The FlexStation™ II³⁸⁴ instrument is designed for 96- and 384-well microplates. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. Lucid Scientific. This reader provides visible. Microplate Reader (Molecular Devices cat. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. Growth medium was then replaced with fatty acid uptake solution. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Both readers were heated to 37ºC prior to setting up the assay. accurately over a wide dynamic range. 96-well: 0. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in. Three kits covering a wide range of dsDNA concentrations. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Plate wash time (300 µl). These robust readers have been placed in labs from Antarctica to. Description. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. #W50-100). • SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Accurate measurements are ensured by automaticThe SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. 24, 48, 96, 384 Well Plates; Wavelength(s): 250 - 850 nm; Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. These instruments are all elegant and simple in their form and function. Five preferred modes for diverse bioresearch applications. Or scan up to 384 wells at any time to determine the best assay conditions. Molecular Devices M5 Plate Reader Plate Calibration Form . i3x Multi-Mode Microplate Reader. The VersaMax reads 96-well microplates. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. Read time* 96 wells < 15 sec. 0200-6262. ± 0. Published Dec 16, 2020 | Updated Dec 16, 2020. Table 1: Categories of heating element design. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. For more sample throughput, the system canMolecular Devices LLC 96 well microplate reader spectrophotometer 96 Well Microplate Reader Spectrophotometer, supplied by Molecular Devices LLC, used in various techniques. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Top or bottom detection is available with a simple click of a button. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. 4% in white 96- and 384-well microplates Sensitivity (ATP-Glow) Optimized 96 wells 3 pM 384 wells 6 pM * With 6 flashes in absorbance and 3 flashes in fluorescence mode and 0. Align the two slots on the bottle holder with the two pegs on the rail. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplateWide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. 2 OD at 200 nm; this decreased to 0. the SpectraMax i3x Multi-Mode Microplate Reader, may also be used for this application. The Perkin Elmer Wallac 1420. maximum-illumination-channels. Taking into account the price differences between each seller and each country. #L6876) • Guanidine hydrochloride (Sigma cat. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 2. Click on the crosshair and move it so that it is centered over the well image. 384 well polystyrene microplates have plate dimensions of 127. Highly selective for dsDNA over ssDNA or RNA. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell viability assays, gfp, picogreen dna. Comparing Luminescence sensitivity of SpectraMax Microplate Readers using ATP Assay | Molecular Devices Author: Molecular DevicesMake the most of your budget with the VersaMax™ ELISA Microplate Reader, our affordable, visible spectrum absorbance reader for 96-well microplates. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. With various on-board features and capabilities, the ThermoMax is. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C. 0 OD Photometric resolution: 0. The system provides options for 20-, 40-,. A multitude of modalities. For more great deals on Microplate readers by Molecular Devices, please browse our Molecular Devices Microplate readers page. Create custom fluorescence assays without having to purchase expensive filter sets. The drawing shows a standard microplate of 15 mm height covered with a lid. Average StDev (mP) 1. A single-mode plate reader is a good starting point. Reading Capabilities: cuvette or microplate. These robust readers have been placed in labs from. /well integration in 96-well luminescence mode and 0. Description. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. CloneMatrix. It measures the optical density (OD) of samples in both 96- and 384-well. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. In May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader . Microplate Readers. (12 and 96 well) all: FMax: all: LMax: all: Macro 96 Well Cell Harvester: all: Micro 96 Well Cell Harvester: all: Mini 96 Well Cell HarvesterSpectraMax L Microplate Reader A flexible, sensitive luminometer for 96- and 384-well microplates Key Features • Unsurpassed sensitivity • Low background and cross-talk • Multi-channel configurations for a 6-fold. The Vmax Kinetic Microplate Reader is designed to measure any of the visual wavelengths so that you can use your substrate of choice. ZERO BIAS - scores, article reviews, protocol conditions and moreThe reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. The FilterMax F3 can read 96-well and 384-well plates. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. OEM Molecular Devices All Microplate Reader, Top r. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Page 11: Reader ComponentsBioTek Synergy HTX Microplate Reader - Absorbance Only Specifications. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Products : General, FilterMax F5 reader. #T-0254) Lysozyme (Sigma cat. Documentation:1. rhodamine-like labels, using specific LED and EX/EM filters • 6- to 1536-well plates 0200. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The accurate positioning of 96-arrayed colonies on rectangular agar plates (in a 96-well scheme) was used to measure each colony with the optics of the plate reader. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit (signal 3X SD of baseline) 8. The kinetic top-read mode of a SpectraMax M3 microplate reader (Molecular Devices, CA, USA). , Sigma cat. Figure 2. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. Injectors Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. #3693; n = 4 for each standard). This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. (FP) configured microplate readers. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. While "plate reader" usually refers to the devices described. It also integrates with the powerful SoftMax Pro Software for. 300 µL of Detergent Compatible Bradford reagent was added to each well. SoftMax Pro Software’s SpectralThe Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. 300 µL of Detergent Compatible Bradford reagent was added to each well. 采用ELISA 方法，对比Molecular Devices 与某进口品牌中等吸附和高等吸附96 孔全透明微孔板，结果表明两种品牌高等吸附板都能结合更多的Protein A，同时Molecular Devices 全透明微孔板有更优的复孔重复性。 The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. 2 nm Wavelength Bandpass 2. It also allows for the elimination of standard curves. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. System setup and calibration are controlled from within SoftMax Pro Software. The CellTiter-Glo assay from Promega uses luciferase. Microplate Capacity Standard 96-well microplates Incubation Temperature control up to 50ºC Temperature uniformity ± 0. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. 384-well: 0. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. University of Reading Simon Lydford: Molecular Devices (UK) Ltd. Published Dec 16, 2020 | Updated Dec 16, 2020. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. buffers for (2) 96-well microplates R8088 CatchPoint cAMP 96-well Fluorescent Assay Kit (Bulk) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (10) 96-well microplates R8089 SpectraMax® i3/i3x Multi-Mode Microplate Reader SpectraMax® Paradigm® FlexStation® 3 Multi-Mode. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. Use standard plate unless you see your type listed. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. The SpectraMax L reader is upgradeable to dual-channel configurations for a 2-fold. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the. adapter plate in drawer of Plate Reader with well A1 in position A1 of the drawer. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. This reliable model allows for accurate wavelength detection in visible spectrum without the need for filters. No accessories are needed and SoftMax® Pro Software. Other features and benefits include: Eliminate changing out filters. This unit offers a wavelength range of 340 nm to 850 nm and a bandwidth of 5 nm. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. . SpectraMax M5eMulti-Mode Microplate Reader (Molecular Devices cat. We would like to show you a description here but the site won’t allow us. 747. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. 030 fmol Fluorescence Polarization (FP). Catalog Number: (10822-512) Supplier: Molecular Devices Description: Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. 54: 1. #G7294). Use the provided 3. All stand-alone instrument functions can be controlled using the software. Fluorescence Microplate Reader Comparison Chart. ZERO BIAS. 4. Despite its form, the Absorbance 96 delivers precise and accurate results at an affordable price. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. 3. The color touch screen provides a visual user interface, making programming fast and intuitive. Microplate Reader, Molecular Devices Optimax, Refurbished. Paradigm: Delta mP. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. F3/F5 Multi-Mode Microplate Readers. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepEight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. Multimode Readers Comparison Chart. After 5, 7, 11, and 13 days. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. 5. ODs were measured in 96 well plates with a plate reader (Molecular Devices, SpectraMax ABS Plus) with two technical replicates of each of.