Chromogenic bacteria black line stain on teeth. We relocated to. Chromogenic bacteria black line stain on teeth

 
 We relocated toChromogenic bacteria black line stain on teeth  chromogenic bacteria, and gingival hemorrhage

If you are looking for teeth whitening dentists. Amalgam will cause a gray/black discoloration directly on the tooth. 7417/CT. dark drinks, including coffee, tea, grape juice, soda, and red wine. Match. caused by compounds, organic chromogens, attached to the pellicle producing a stain. Some examples of the most common extrisic stain are ? Coffee, tobacco, yellow, green and black line stain. Black Stain. How is the composition of black line stain different from supraginival calculus? in supragingival calculus, cocci predominate. were isolated from the dental plaque of a child diagnosed with black stain. results from oral uncleanliness, chromogenic bacteria fungi and blood pigments. Various factors can cause the teeth to become. The color of the stain tells a lot. My son also had chromogenic bacteria on his teeth for several years and we had to go to the dentist 2-3 times a year to remove it. Upgrade to remove ads. Can be fixed. 46 The characteristic black. the etiology of this stain is the result of uncleanliness, chromogenic bacteria, and gingival hemorrhage. extrinsicclinical- light or yellowish green to dark green, line or crescent shape, facial surfaces (cervical 3rd of max anterior), area beneath stain could be decalcified, may be intrinsic, Skinner solution may be helpful in removal etiology- chromogenic bacteria and fungi, decomposed hemoglobin from hemorrhage, poor oral hygiene (materia alba and food. Study with Quizlet and memorize flashcards containing terms like Discoloration of the teeth and restorations occur in 3 general ways, Significance of Stains, Classification of Stains and more. a preparation in liquid, tablet, or lozenge form that contains a dye or other coloring agent. Stain usually caused by food pigments on dental biofilm, common to all ages, more evident when care is neglected, appears a dull yellow. -Often found in coffee, tea, and chromogenic bacteria. stain forms on the teeth in the pellicle after repeated use of stannous fluoride gel. Extrinsic dental black stain (BS) can appear as a complete or incomplete black line on the buccal and/or lingual surfaces of the teeth near the gingival margin 1. Iron containing oral solutions. Learn vocabulary, terms, and more with flashcards, games, and other study tools. -occurs on external surfaces of teeth-removed by scaling/polishing. LOCATION-Extrinsic/Intrinsic 2. Extrinsic black dental staining is an external dental discoloration of bacterial origin, considered a special form of dental plaque. 1038/s41415-022-4345-0. Area in. Red-black stain. Stains that occur on the external surfaces of the teeth may be removed by polishing, scaling, and/or toothbrushing Ex: Coffee, tea, smokingBLIS M18 was shown in a recent in vitro study to inhibit two of the bacterial species that cause the black stains to form on teeth. an endogenous tooth stain originates from _____ the tooth. external-stain adhering to tooth surfaces-stain contained within calculus or soft depositsBlack Line Stains *Lingual surface *More often in females than males, more often in children than adult *Chromogenic bacteria. Preprocedure rinse with Chloroxadine gluconate. The stains look like pigmented black lines along the gumline and are due to the presence of a form of bacterial plaque living in the child’s mouth. Study with Quizlet and memorize flashcards containing terms like Black line stain, Black line stain, Black line stain and more. chronologic. We relocated to. It can turn grayish-black over time due to exposure to certain substances that stain it. intensity of color. -Chlorhexidine rinse. Black stain (BS) manifests as dots or black lines distributed in parallel to the gingival margin of teeth commonly in the cervical third, and is firmly attached to the. -Most frequently found on lingual and proximal surfaces of maxillary. 3. on the outside surface of the tooth. light brown to dark leathery brown or black. are always intrinsic. Black tooth stain is a type of extrinsic discoloration of the tooth. Black stain (BS) manifests as dots or black lines distributed in parallel to the gingival margin of teeth commonly in the cervical third, and is firmly attached to the enamel, thereby complicating. This type of stain seems to be more prevalent in children. Test. discoloration most often found in children; found on the facial surface of the maxillary anterior teeth at the cervical third, containing chromogenic bacteria and fungi; varies from light to dark green or yellowish-green. 2 AU? (An AU or astronomical unit is Earth's average distance from the sun; the sun and planets are represented by points of identical. Reforms after removal. In addition, they tend to reappear after their removal 1. Common dental black stain treatments include scaling and polishing the teeth [6,9]. Study with Quizlet and memorize flashcards containing terms like Extrinsic Stains, Yellow and Brown Stains, Tobacco Stains and more. Test. Test. Extrinsic stain that may appear as a small curved line following the contours of the facial gingival crest, Smeared irregularly over the whole facial surface,Study with Quizlet and memorize flashcards containing terms like Ways to Acquire Stain, Classification of Stain, Green Stain and more. chromogenic bacteria. orange and red stains. 033) and regular application of fluoride (P = 0. Decomposed hemoglobin. endogenous- develop or originate from within the tooth. Black line stain. Extrinsic stain, fine line at gingival third, made of gram positive rods and other microorganisms. Intrinsic. smeared irregularly over facial surface, 3. Erinhrmt. It may occur on primary or permanent teeth. dentinogenesis imperfecta. It’ll help prevent some staining. Light to leathery stain that may be in calculus and is difficult to polish off. No thickness -Found on cervical 1/3 near GM -follows gingival contour -mostly on lingual -Occurs most on max. Metallic dusts (copper), or marijuana. 99/year. This is a thin black line, firmly attached on tooth surface. -pulpless or traumatized teeth-developmental issues -amelogenesis. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Coronal Polishing. -yellow stain-green stain-black line stain-tobacco stain-brown-betel leaf- Enamel under stain is sometimes demineralized as a result of cariogenic biofilm - Primarily on facial tooth surfaces; most frequently facial cervical third of maxillary anterior teeth - Chromogenic bacteria and fungi, decomposed hemoglobin, and inorganic elements - Primarily found in childhood but can occur at any ageIntroduction. Black stain is characterized as a dark line or an incomplete coalescence of dark dots localized on the cervical third of the tooth. 42 ± 0. Now, they are a common anaerobic bacteria and germs on your teeth capable of causing cavities and disease. – whitening toothpastes. Rare. Study with Quizlet and memorize flashcards containing terms like adhere to tooth present in biofilm or calculus in tooth structure, extrinsic stain, intrinsic stain and more. Abstract. Colour: is a combination of, Chroma (type of colour), value (intensity of colour –the relativity of lightness or darkness) and hue (a dimension of colour). Location: follows gingival martini about 1mm from margin, cervical 1/3 Cause: commonly. extrinsic stains C. Comparators: children without dental black stains. orange and red stains. predisposing factors. This recurrence may be attributed to. -Chromogenic bacteria and fungi-Gingival hemorrhage - bacteria decompose hemoglobin-Poor oral hygiene. Study stains and fluoride flashcards. chromogenic bacteria & fungi and gingival hemorrhage. originates from chromogenic bacteria (which causes the color) metallic stain. It looks like pigmented black lines along the gumline. Other brown stains VI. Its aetiology is not completely understood, although the black pigmentation is likely to be an insoluble ferric salt precipitate, produced by. 3) Chromogenic bacteria 4) Any age, Primarily childhood; permanent or primary teeth 5) Depends on home care 6) Poor oral hygiene 7) Often demineralized teeth under green stain, not wise to scale these teeth, should polish instead 8) Check what drugs, where they work, or do they smoke marijuanaBlack line stain is usually related to a clean mouth. Guest over a year ago. metallic . false. anteriors. Stain, biofilm, calculus. Outcomes: Occurrence of dental caries, number of teeth affected, number of surfaces affected. 10. Chromogenic bacteria, fungi, decomposed hemoglobin and inorganic elements. 1. I did read that people with black line stain tend to have excellent dental hygiene. common in children . Match. gingivalis, contributed to the black pigmentation. Key points. These stains are firmly attached. A microbiological analysis of BS samples from children’s teeth highlighted the presence and importance of chromogenic bacteria such as Prevotella melaninogenica, Actinomyces israelii and. orange stain-extrinsic-chromogenic bacteria in plaque; associated with poor oral hygiene; typically located on anterior teeth. BTS is clinically diagnosed based on the deposition of dark-colored lines or dots parallel to the gingival. If given during tooth development or during the. The condition is more common in children than adults. 4 methods of stain removal. Cigarette smoking has been associated with a number of diseases, and all of them are far worse than simple staining of the teeth. It is likely that the cause will be more than one. Green stain. Can be chromogenic bacteria or color producing bacteria due to food pigment. Black stain (BS) is considered a special form of dental plaque unique for its insoluble iron salts and high calcium and phosphate contents. 2019. Abstract. always intrinsic and usually are discolorations of the dentin reflected through the enamel. Over the last century, the etiology of black stain has been the subject of much debate. primarily facial often extends to proximal, most frequently facial gingival third of maxillary anterior teeth chromogenic bacteria and fungi. The presence of pigmentation from chromogenic bacteria is a relatively common in day-to-day practice finding. Read more. Exposure: black stains of any amount or extent related to primary teeth. Study with Quizlet and memorize flashcards containing terms like Extrinsic Stain, Example of chromogenic bacteria, Black line stain and more. Chromogenic Bacteria 2. Chromogenic bacteria cause stains, typically at the gingival margin of the tooth. Black line stain. Log in. Tabacco Stainyellow, green, black line, tobacco, brown, orange and red and metallic stains. Tetracycline Discoloration. Learn vocabulary, terms, and more with flashcards, games, and other study tools. In some. -Often found in coffee, tea, and chromogenic bacteria. Removed by tooth-brushing, scaling, and/or use of abrasive polish and rubber cup. There's no guarantee that the issue won't return in the future, but removing this type of discoloration couldn't be any easier. ) Small curved line following contour of gingival crest 2. Study with Quizlet and memorize flashcards containing terms like Medical and dental history, Extrinsic stain, Yellow stain and more. Stains occur from dyes in food, beverages, tobacco, mouth rinses, exposure to metallic compounds, the presence of chromogenic bacteria (colour-producing bacteria) and poor oral hygiene. Clean 2 to 3 teeth then rinse with. Many other recent studies also support an inverse relationship. Very interesting. Pigment producing bacteria are called. In most cases, black line stains may be detected at the gumline. Caries prevalence was 49. These stains are firmly attached to the tooth and are difficult to remove with conventional toothbrush and toothpaste. Poor dental hygiene and tartar buildup can also result in dark stains on the teeth. Appearance: highly pigmented black or dark brown calculus (like stain) Occurrence: more common in females; adults or children; frequently found in clean mouths, recurs despite good OH Distribution: cervical 1/3 of teeth; follows contour of gingiva approx. Both the statement and the reason are correct, and they are related. black, green, orange. Chapter 22: Dental Stains and Discolorations. 2 mathrm{AU} = 0. What are the different colors of Metallic Stains?. PLAY. The color of the stain tells a lot about how the discoloration occurred: Orange-red: chromogenic bacteria, chromic acid/copper chemicals; Yellow: heavy dental biofilm/calculusPrevious literature shows that children with dental black stain might be less susceptible to dental caries. Chromogenic Bacteria stain on teeth . Only $35. Black line stain. -Teeth are frequently clean and shiny with a tendency to lower incidence of caries. metals and metallic salts may be inhaled in industrial settings or taken orally in certain drugs, or comprise part of the material used to restore teeth. Subjects. It can be diagnosed as pigmented, dark lines parallel to the gingival margin or as incomplete coalescence of dark dots rarely extending beyond the cervical third of the crown. does black line stain appear on MX. Match. Learn. Children are especially prone to orange tooth stains, usually as a result of improper or infrequent brushing. The stains are absorbed onto tooth surface deposits such as plaque bacteria (white, sticky substance) that accumulate on teeth, especially around the gum line. Introduction. Source. . Staining due to tobacco. It could also mean that your child is a mouth breather. Create. 2 other stains caused by Chromogenic bacteria. Gram positive rods-Actimomyces species Bacteriodes melaninogenicus. SOURCE-comes from chromogenic bacteria and hemoglobin in bacteria-occurs in primary teeth Black line stain where is the stain? caries and fissures? -GM is demarcated from the brown-black stains -low caries -black in fissuresStudy with Quizlet and memorize flashcards containing terms like Amelogenesis Imperfecta, Chlorophyll, Chromogenic and more. Sign up. Dhruv Gupta, MD answered this Chromogenic Stains On Your Teeth: Causes And Solutions . exogenous stains B. Coronal Polishing. Key points. In a companion clinical trial in children, BLIS M18 was shown to discourage the recurrence of the black stains after removal by professional cleaning. 9%. These bacteria create a brownish film on your teeth, which turns black with time. This type of pigmentation is a special form of. Learn. Old plaque can remain stuck between teeth, in grooves, and along the gum line. Study with Quizlet and memorize flashcards containing terms like extrinsic stain, intrinsic stain, exogenous and more. -Most frequently found on lingual and proximal surfaces of maxillary. The results suggest that the black material is a ferric salt, probably. For clinicians, most interesting external tooth discoloration in children has been the black discoloration, which is more frequently observed compared to the other types. Black Line Stain, Extrinsic. dark pink= plaque. permanent teeth [1]. Upgrade to remove ads. Effect of bleaching agents on black tooth stains caused by chromogenic bacteria: 10 years follow-up case report. Yellow. stain contained within calculus & soft deposits 3. Extrinsic dental black stain (BS) can appear as a complete or incomplete black line on the buccal and/or lingual surfaces of the teeth near the gingival margin 1. Cigarette smoking has been associated with a number of diseases, and all of them are far worse than simple staining of the teeth. caused by external source. Stains contained in calculus and soft deposits. I wonder if it's because this bacteria is immune to good hygiene, so you're killing the bacteria that don't cause this stain,, allowing the black line stain bacteria to thrive. Orange and red stains can be. These deposits can cause tooth discoloration to black or even green and orange in children with poor oral hygiene. 2019. -Stains adhere directly to the tooth. Fluorosis can affect any permanent or primary teeth that are exposed to excess fluoride systemically during development. oral. Light or yellowish green to very dark green. PLAY. Study with Quizlet and memorize flashcards containing terms like Medical and dental history, Extrinsic stain, Yellow stain and more. Log in. Calcium is beneficial for teeth as it strengthens and remineralizes enamel. J Vis Exp. DESCRIBE THE STAINS CAUSED FROM METALLIC DUST OF INDUSTRY . A dietary cause like tea and coffee drinking can cause chromogenic stains if it is coupled with teeth that are inherently rougher. Extrinsic staining of the teeth can make the teeth look gray, brown, yellow, orange, or green these stains can be due to: Food stains such as after eating blueberries. - Black line stain occurs despite regular personal care. Microorganisms: gram + rods in an intermicrobial substance. Black stain (BS) is a specific type of extrinsic dyschromia caused by the presence of chromogenic bacteria in the oral cavity, which occurs more frequently in children, but may also occur in adults. Intrinsic. }Composed of chromogenic bacteria, fungi, and decomposed hemoglobin. something that occurred while. Gram + bacteria (actinomyces) binding to pellicle that turns black due to this. composition -Green Stain (Extrinsic) - Chromogenic bacteria ,gingival hemorrhage and fungi, by blood pigment from decomposed hemoglobin, and. Sign up. Yellow green black line tobacco chlorhexidine . other types of stains A light to very dark green extrinsic stain appearing primarily on the labial surface of the teeth due to inadequate daily removal cleaning, chromogenic bacterial deposits or decomposed hemoglobin. Start studying Chapter 22: Dental Stains and Discoloration. black-line stains are compared to _____ and may be detected by gentile application of an explorer. Most common stain in children. , (1974) reported that chromogenic bacteria were proposed as etiological factor in production of black stains. Dental Accretions: Hard and Soft Deposits. Stains, Selective Polishing, Disclosing study guide by ssilvia5 includes 20 questions covering vocabulary, terms and more. Then the antibiotic killed it off. It can be diagnosed as pigmented,. Study with Quizlet and memorize flashcards containing terms like What are the ways stains can adhere to the teeth?, Gingival disease and tooth decay are related to what?, True/false extrinsic stains can be removed, as they are on the external surface of the tooth and more. Chlorhexidine; antibiofilm. It started a few years ago. So far there is no significant and safe treatment available. Black line stain. Can often be removed by tooth brushing, scaling & polishing. Restorative procedures, such as veneers or composite bonding, achieve a better aesthetic result for clients with tetracycline-stained teeth because gray-blue stains are resistant to tooth bleaching. chromogenic bacteria and fungi; decomposed hemoglobin from gingival hemorrhage;. The presence of black line stains indicates the presence of chromogenic bacteria. These deposits can cause tooth discoloration to black or even green and. I did read that people with black line stain tend to have excellent dental hygiene. Green stain is most commonly found where? Black staining of teeth due to Chromogenic (coloured) bacteria can be a source of poor self-esteem for patients, especially children. discoloration of teeth and restorations occur in 3 ways. You may notice a thin black line, or a dotted line, on your teeth, mostly near your gumline. This could lead to a significant reduction in the amount of black extrinsic stains. May 30, 2020 What Causes Black Line Stains? by BURST Oral Care There are droves of people that are frustrated with stains on their teeth. Learn vocabulary, terms, and more with flashcards, games, and other study tools. When gum disease is present, it can lead to inflammation and infection that cause the gums to darken in color. What is black line stain composed of? Gram (+) actinomycetes. Only way to remove it is by having a cleaning done by a hygienist. chewing substance that stains teeth. algae, and some bacteria. along a small curved line following the facial of the gingival crest, 2. Yellow: Yellow discoloration might be the most common. It could also mean that your child is a mouth breather. Endogenous - by location. Linked to chromogenic bacteria & requires scaling to remove. What caused the green stain, things/food can't get out so it leaves a green stain. Sign up. Green stain. originates from sources outside the tooth - tobaccoStains can be all shapes and sizes: spots, patches, a line that crosses the tooth, or a dark ring that circles between teeth. Black tooth stain (BTS) is a common condition characterized by an extrinsic discoloration of teeth due to the deposition of bacterial plaque enriched in calcium phosphate minerals and insoluble iron-containing compounds (1, 2). What causes chromogenic bacteria on teeth? Orange Stained Teeth. Drugs that could cause metallic stain - iron sulfide - potassium permaganate. 2. Study with Quizlet and memorize flashcards containing terms like What 3 ways do stain adhere to teeth?, What are gingival disease and tooth decay related to?, What does stain play a role in? and more. Brownish stain on the tongue and tooth surfaces may result, 4. grow large enough to touch and unite. fungi, chromogenic bacteria, biofilm and acquired pellicle, matallic and marijuana can stain it as well. Can be found on the lingual & facial surfaces of the anterior teeth near the gingival margin. Of all the children involved, 6. Extrinsic dental black stain (BS) can appear as a complete or incomplete black line on the buccal and/or lingual surfaces of the teeth near the gingival margin 1. Baby teeth can develop a type of bacteria that causes brownish to black extrinsic stains on the surface of teeth. Home. in MCF-7 cell line: MTT assay. Staining by Color. Black stains associated with chromogenic bacteria are a common dental finding. The dental black stain (BS) is formed due to the colonisation of chromogenic bacteria, which is commonly reported in the population at the pre-puberty age. It presents as an incomplete line of dark dots formed at the cervical third of the tooth, typically following the gingival margin and not extending to the proximal areas. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Exogenous staining. Actinomyces stain. mesenteric line, brown stain, black extrinsic tooth stain, black line stain or pigmented dental plaque. stain within tooth structure or restorative material (1 & 2 can be removed by scaling or polishing) Importance of stain removal. Chromogenic. No correlation was observed between the presence of black stains and age and gender of children. 7 black-stained teeth. Chromogenic Bacteria. Stains can provide _____. Stain Pigmented substance, either on the surface of the tooth or within the tooth, that results in an abnormal discoloration of a tooth a) it can adhere directly to tooth surfaces b) it can be contained within calculus or soft deposits c) it can be incorporated in the tooth surfaceStudy with Quizlet and memorize flashcards containing terms like how dental stains occur:, classification of stains:, exogenous stain and more. continuous or interrupted fine line; forms along gingival margin; teeth clean and shiny with lower decay rate. While the issue is most common in children, rarely, it may also occur in adults. The discoloration appears in lines or dots. -specialized type of brown stain-caused by excessive fluoride intake-if it occurs during tooth development, the result is permanent staining-mild cases appear as white spots or dots in the enamel-severe cases appear as pitting and discolouration of the enamel-severity depends on the amount of fluoride ingestion and age it occurredStudy with Quizlet and memorize flashcards containing terms like What can certain stains evaluate of a person's oral health?, stain that is on the external surface of the tooth, stain that occurs within the tooth surface and more. tobacco stainWhat are the organic components of calculus? non-vital microorganisms, desqaumated epithelial cells, leukocytes, mucin from saliva, cholesterol phospholipids, fatty and amino acids. stain incorporated within the tooth and structure or the restorative materal. intrinsic staining-occur w/in tooth-NOT removed by scaling/polishing. They can be removed by professionally cleaning the tooth’s surface with delicate techniques and tools which do not scratch it, such as: – ultrasounds. The stain is composed of ferric sulfide and is formed by the reaction between hydrogen sulfide produced by bacterial action and iron in the saliva and gingival exudates. Gram positive rods embedded in an intermicrobial substance. posterior teeth -often found in people with excellent oral hygiene. Terms in this set (15) Extrinsic. That black line may result from having stain-forming bacteria in your mouth. I had this severe problem of black stains caused by chromogenic bacteria on my teeth. -composed of chromogenic bacteria, fungi, and decomposed hemoglobin and inorganic elements. For tartar. Search. Some scientific research suggests that it is associated with iron compounds (ferric sulfide) and betel leaf chewing habits. 2 different categories of sources. Study with Quizlet and memorize flashcards containing terms like Dental stains, Classify stain by. endogenous. black line stain - gram and rods bacteria - lingual and proximal surfaces of maxillary posterior teeth and occlusal pits - slightly above gingiva - feels like "speed bump" tobacco stains}Embedded in biofilm}Can exist as a curved line following contour of facial gingiva, streaked through grooves in enamel, or covering cervical 1/3 to ½ of facial surfaces. - C: Green stain, which is also found in clients with poor oral hygiene, occurs near the cervical third of the teeth - black stain (black-line stain) can occur in clients with meticulous oral hygiene. Example of chromogenic bacteria. 59 terms · Most stains occur on _______ of the teeth → The cervical 1/3, T/F: Stains incorporated within the tooth structure can be removed. Expert solutions. Black line stain IV. Black tooth stain (BTS) is a common condition characterized by an extrinsic discoloration of teeth due to the deposition of bacterial plaque enriched in calcium phosphate minerals and insoluble iron-containing compounds (1, 2). 4%. It differs in etiology, appearance, composition, location and severity. Brown stain resulting from foodstuffs such as coffee or tea E. producing color or pigment. 3. Black line stain is. 2163 Abstract Among the various pathologies of the oral cavity, the formation of "unsightly black spots" on the surface of the tooth, universally known as Black Stain (BS) has recently been acquiring more interest. exogenous tooth stains can over time become _____. -primarily facial -chromogenic bacteria and fungi -Occur at any age -results from bad oral health care, chromogenic. One of the major causes of teeth blackening are the chromogenic bacteria which form biofilm in the teeth. Preventing stained teeth. intrinsic. -chromogenic bacteria in plaque or enamel cuticle-fungi-decomposed hemoglobin (bleeding)-inorganic elements. gram positive bacteria; typically located on cervical 1/3 of facials and linguals. Endogenous. Spell. . Outcomes: Occurrence of dental caries, number of teeth affected, number of surfaces affected. calculus formation. Start studying N2- Stains and Discolorations. 67 It starts as early as age 2 and can signi fi cantly affect the parents and have an in fl uence on the child ’ s personalit y as well as theStudy POC Stains flashcards. metallic dusts, green teas, drugs (weed). brown tobacco stain. Both the statement and the reason are correct, but they are not related. Dentinogenisis imperfecta. Colonies of these orange or green bacteria usually mean that you need to do a better job of cleaning your child's teeth. Study with Quizlet and memorize flashcards containing terms like Stains adhere to the teeth in 3 ways, Significance, Extrinsic and more. (3) Slots et al. Match. Classication Black stain can be classied based on the surface area of the tooth a˚ected. Colonies of these orange or green bacteria usually mean that you need to do a better job of cleaning your child's teeth. Upgrade to remove ads. Flashcards. Sometimes grey, brown, green and even orange! These bacteria produce hydrogen sulphide that reacts with saliva in mouth and form black stains on your teeth. Home. 1,3 The antibiotics alter the normal flora, allowing chromogenic bacteria to grow and. 2. Results from poor oral hygiene, dental biofilm retention, chromogenic bacteria, and gingival hemorrhage. Black-line stain. (2) Chromogenic bacteria-e.