1. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 0 and 0. 73. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Add to Cart. SpectraMax® Aquamax. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Molecular Devices Spectramax M5 Microplate Reader. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Learn how to set up protocols, customize reports, export results, and more with this PDF document. TNF standard curve. Spectramax Multi-Mode Microplate Reader Performance Specifications. Access this by going to Help >. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. . The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. STAKMAX. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. The SpectraMax® ABS Plus Microplate Reader can run . FlexStation™ II. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. When a FlexStation® 3 Multi-Mode Microplate Reader. Ltd. 2. All associated StakMax® stackers. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The dual monochromator optics allow the. IMAP FP Settings in SoftMax Pro M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. 0 OD for earlier models. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. Molecular Devices | Life Science Applications | Bioanalytical. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. Filterless readers provide consistent results with steady temperature regulation. Microplate Readers are modular and . Five. 2. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. The cuvette must be in place when you read the plate. SpectraMax iD5 Multi-Mode Microplate Reader. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. 05% Tween 20 using a Nunc ELISA plate washer. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. We would like to show you a description here but the site won’t allow us. View the Molecular Devices website. Most papers they cultured bacteria to OD600>0. Examples: “Press carefully to engage,” “ Do not. Category 2 and 3 microplate readers use gradient heating as well. SpectraMax M3 M4 M5 M5e SpectraTest FL1 revA. 313 reference gain check fail. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. M3 M4 M5 M5e LM1 v<nnn>. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. If you do not have an iLab account you must first make one: The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. We use Spectramax M5 microplate reader in our lab. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 6. For readers without a cuvette port, these options are not shown in the software. Temperature variation ± 0. 1. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. Read one sample or up to 384 inThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Before you Begin Materials Required but Not Provided • Fresh, anhydrous. The chamber contains springs that position the cuvette in the proper alignment for a read. Shop now. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Figure 4: Transcreener ADP. 81). 40266 Email: drugdiscoverytech@lifetech. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 312 gain calibration failed. Detailed specifications of each model reader are reported in Section 7, “Specifications”. When a SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 05 3. SpectraMax M3, M4, M5 and M5e Multi-Mode Microplate Readers User Guide Fluorescence Microplate Reader Comparison Chart. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Page 1 of 8 . free tracer decreases, resulting in an. Supersedes 1. Microplate reader settings. The system. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. Then, after H 2 O 2 was added, the fluorescence was measured for each time period at 488 nm (excitation) and 525 nm (emission) wavelengths on a SpectraMax M5 microplate reader. 2. No additional manuals are available. microplate reader. 1–0. 0 out of 5 $ 3,799. After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. You should get comparable results from top and bottom reads using EC50's to compare reads. Figure 1. iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. 314 low lamp level warning. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. When a feature or capability applies to only certain readers, this exception is noted. Documentation Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The spectrophotometric. 00 USD. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Figure 3. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. reader in the visible range between 405–750 nm with NIST and NMI. Expanded wavelength coverage. ATG provides repair, maintenance, and contract services for laboratory research and bioprocess instruments. Add to Cart. We would like to show you a description here but the site won’t allow us. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Anne T. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Maximum signal with lowest background. TR-FRET assay principle. Our absorbance plate readers feature our PathCheck Sensor. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. SpectraMax® ABS. es •. The plate reader can measure multiple readouts, including UV and visible absorbance. The cells were incubated with compounds at the indicated doses for 1 h. Nontreated cells in DMEM medium were used as a control. Fluorescence from the Alamar blue® assay was detected by SpectraMax M5 microplate reader (Molecular Device Inc. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SpectraMax M5 reader settings are shown. . Compact and tunable absorbance microplate readers DATA SHEET. 00 USD. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Then, the fluorescence change rates (F 0 − F)/F 0 were calculated, and the selectivity histogram. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Five preferred detection modes. Qualify the fluorescence performance of the SpectraMax . Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Turn on the microplate reader. M3-M5e_manual. Step 2. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. 5. The percentage of viable cells for each treatment group was calculated by adjusting the untreated control group to 100%. Shop now. Standard features include a. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Urea-induced unfolding of rA1AT-WT and the Q9N mutant was measured using a SpectraMax. ® reader to the computer. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. 7. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. Western blotting assays were conducted according to previous studies . • Tel. Table 1. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. 2. SoftMax Pro Software has been integrated by many leading robotics andFor readers without a cuvette port, simply check the box next to ‘PathCheck’. spr. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. • Tel. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidethe reader’s stated range. The optical density (OD) was measured at 450 nm with a SpectraMax M5 microplate reader (Molecular Devices). 7°C and the bottom to 36. The drawing shows a standard microplate of 15 mm height covered with a lid. edu) to be added as a trained user in iLab. 1. We use Spectramax M5 microplate reader in our lab. 4 0. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Microplate Reader Shelf. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. Recommended Optics Wavelength (nm) Wavelength. Fluorescence Microplate Reader Comparison Chart. ,. The following document is intended to demonstrate setup of this instrument. Insert the filled microplate into the drawer, matching well A1 with position A1 in the drawer. temperature and fluorescence was measured at Ex/Em 490/585 nm using a Molecular Devices SpectraMax M5 microplate reader. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. 1. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. flexibility of monochromator-based systems. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. The absorbance value of each reaction solution was measured at 405 nm by a Molecular Device SpectraMax M5 microplate reader (Sunnyvale, CA, USA). SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. ATP standard curves read on SpectraMax microplate readers. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. It measures the optical density (OD) of samples in a microplate or in a cuvette at aThe cells were then washed three times with PBS, and the fluorescent intensity of the cells was measured with a SpectraMax M5 microplate reader (Molecular Devices, USA) at an excitation wavelength of 488 nm and emission wavelength of 525 nm. 6 0. SpectraMax 250 User. Description: This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with t. 00 USD. Figure 1. 7 cells were co-transfected with 0. ) by nonlinear regression using. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. KEY FEATURES • Five. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The Multiskan Sky microplate reader is an absorbance plate reader. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Fluorescence Microplate Readers. The SpectraMax M2 and M2e are versatile and reliable. used. After two more rinses with Hank’s solution, fluorescence intensity of the red/green ratio was determined on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at an excitation of 490 nm and emission of 530 nm (green fluorescent monomers) and 590 nm (red fluorescent aggregates) respectively. 1step assay with SpectraMax microplate readers. Or scan up to 384 wells at any time to determine the best assay conditions. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 6-384 (96- and 384-well with pipetting) 6-384. Fill a clean plate with water. The raw data from each individual experiment were normalized to vehicle-treated HK-2. SoftMax Pro. e. Microplate Reader, Multimode Tecan Infinite M200 Pro Nanoquant M Nano+ Dual-Mode Microplate Reader 30050303. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. 2. both cuvette-based and microplate reader applications on the same instrument. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. M3-M5e_manual. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. View the Molecular Devices website. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. The following document is intended to demonstrate setup of this instrument. Published Dec 16, 2020 | Updated Dec 16, 2020. . Note: In this user guide, all references to SpectraMax Multi-Mode. 0 μL) instead of the enzyme solution was used as the sample blank group. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. 4. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. The absorbance was measured at 490 nm using a SpectraMax® M5 microplate reader (Molecular Devices, USA). SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Bottom-reading will also give you higher RFU's (and the appearance of high sensitivity) because the polystyrene will fluoresce a bit. Add to Cart. The SpectraMax® iD3 Multi Mode Microplate . The guide also covers the software features, the specifications, and the accessories of the systems. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. Here the delta mP was 336, and Z’ factor was 0. 96-384. 304 signal level saturation. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. TR-FRET assay. Example: [Drawer]. Standard SDS-PAGE was performed with a 10% (w/v) separating gel and a 4% stacking gel. For more sample throughput, the system can Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. Figure 1. l. SpectraMax iD3, iD5, i3x, i3, M3, M4, M5, M5e, SpectraMax L, 6186 Luminescence FlexStation 3. Fresh cecum content samples were collected for 16S rRNA sequencing by Shanghai Majorbio Bio-Pharm Technology Co. Flexible for 6-well to 384-well microplates and cuvettes. 59 6. Path Check Pathlength Measurement SpectraMax Spectrofluorometers. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. . SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. Microplate Reader, MultimodeProducts : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). When a feature or capability applies to only certain readers, this exception is noted. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. e. Page 28 Chapter 5: Read Modes and Read Types 1. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The Spectr. The samples were boiled for 5 min with 1 ×. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. SpectraMax M5e Multi-Mode Microplate Reader. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. : 2 Oct 12 Page 2 of 10 Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Thank you!. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Make sure the microplate is flat against. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Between steps, reactions were rinsed 3 times with TBS + 0. 87. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. A control experiment of the activity of the proposed enzyme/ZIF-8A particles without peptides against protease digestion was also performed. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. 7 cells were pretreated with SG (0, 25, 50, and 100 μg/mL). view more . The assay window spanned four orders of magnitude. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. If you do not have an iLab account you must first make one: would like to show you a description here but the site won’t allow us. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. 1. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). 7 cells (6 × 10 5 cells/well in 6-well plates) were pretreated with of 3,3′,4,5′-TMS or 3,4′,5-TMS at 10 and 50 µM for 4 h, followed by the addition of LPS (1 µg/mL) for 12 h. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Microplate Reader Documentation Version No. Fill a clean plate with water. Compatible automation solutions for SpectraMax Readers. The cells were then treated to induce apoptosis. Or, with the reader specifications noted from the Instrument Description section of your reader's. We would like to show you a description here but the site won’t allow us. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Biotek FLx800 Fluorescence Microplate Reader; Related products-20%. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). The inset, using the same data set, highlights the sensitivity of the assay at low levels of H 2O 2. free tracer decreases, resulting in. Data are normalized to vehicle-treated. After 16 hr of treatment, cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. 305 reference level saturation. Fluorescence intensity was detected by. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. E Models Only) 69. The chamber contains springs that position the cuvette in the proper alignment for a read. edu) to be added as a trained user in iLab. SpectraMax Plus 384 (Molecular Devices) [4 units] UV and visible absorbance monochromator-based microplate reader SpectraMax M5 (Molecular Devices) [2 units] multi-label monochromator and filter-based microplate reader for UV and visible absorbance, fluorescence intensity, time-resolved fluorescence, fluorescence. FlexStation™ III. The CellTiter-Glo assay from Promega uses luciferase. It cannot be used for luminescence measurements. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Introduction. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. SpectraMax Lmicroplate luminometer.