2) If you only wanted to produce cDNA from a subset of all mRNA, then. USD $1207. 10µl fractions of each reverse transcription reaction product, representing 100ng to 1fg of. Life Science Grants. 205110, 205111and 205113) should be stored immediately upon receipt at –30 to –15°C in a constant-temperature freezer. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. OmniScripts allow you to craft dynamic customer. Both enzymes exhibit a high affinity for RNA, facilitating transcription through secondary structures that may inhibit other reverse transcriptases. All components that are required for fast and efficient reverse transcription are provided with the QuantiTect. Sensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). For reverse transcription of 50 ng to 2 micro g RNA per reaction for end-point PCR. 1 ml 1. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRReal-time RT-PCR A OneStep RT-PCR Kitb was used with a 25-#l reaction volume. Vlocity Intelligence. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. The ideal reverse transcriptase is robust (highly active under a variety of conditions) and converts all primed RNA within a sample to cDNA, regardless of its abundance, length or secondary structure. All reactions took place for 1h at 37°C in a final volume of 40 μl containing 1 μg total RNA in the presence of 20 units RNase inhibitor (RNasin® Ribonuclease Inhibitor, Promega), 1 μg random hexamers (Microsynth), 4 μl 10. There are elements for: User. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Balanced mixture of enzymes with optimized reverse-transcription buffer The QIAGEN One-Step RT-PCR Kit provides a blend of Sensiscript and Omniscript Reverse Transcriptases, HotStarTaq DNA Polymerase, QIAGEN OneStep RT-PCR Buffer, a dNTP mix, and Q-Solution, a novel additive that enables efficient amplification of “difficult” (e. DSL Service Product. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per. There-fore, 2,500 copies (1 fg) was chosen as the relatively small. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. C, Real-time RT-PCR for IL-17 and IL-10 expression in wild-type, DKO, and TKO CD4 + CD25 − T cells after 2 d of culture with anti-CD3 and anti-CD28, combined with IL-6, IL-27, or IL-6 plus IL-27. It is crucial to recognize and prevent potential issues with cDNA synthesis in order to maintain the reliability of experimental. 4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Reverse transcription is a powerful tool for creating complementary DNA (cDNA) from RNA, which can then be used in a variety of downstream applications for studying RNA. OmniStudio. The unique combination of Omniscript and Sensiscript Reverse Transcriptases, with their high affinity for RNA templates, ensures highly efficient and sensitive transcription of RNA amounts from as little as 1 pg up to 2 µg. Omniscript and Sensiscript QuantiFast RT Mix contains an optimized Omniscript and Sensiscript blend. For reverse transcription of 50 ng to 2 μg RNA per reactivity for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROverall, my experience with Qiagen’s OmniScript RT Kit has been good. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRNxtScript 2G RT is the latest member of the family and pushes the temperature of the RT step to new levels: no loss of activity even at 70°C. RT activity is critical for many basic techniques including real-time and endpoint RT-PCR, labeled-probe generation for microarrays and cDNA cloning. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. RNA from Svalbard ptarmigan brain samples was extracted using TriReagent (Sigma-Aldrich) and the extracted RNA was converted into cDNA using the Omniscript RT kit from QIAGEN. A 20 μl RT reaction included 2 μg Universal reference RNA (Stratagen), 1 μM Oligo-dT primer, 2 μl of 10× RT buffer, 0. RT-PCR. 1 mL RNase-free. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe reverse transcription was achieved using Omniscript RT Kit (Qiagen), according to manufacturer’s instructions. For the reverse-transcription step, both Omniscript and Sensiscript are functionalized with an RT-blocker, which keeps them in an inactive state and prevents nonspecific transcription at ambient temperature. 2800. 381KB. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Sensiscript RT is optimized for use with very small amounts of RNA (<50 ng), such as for single-cell RT-PCR or analysis of small biopsies. For reverse transcription of 50 ng to 2 μg RNA per reaction fork end-point PCR Omniscript Reverse Transcription（RT）Kitは、各反応あたり50 ngから2 µgのRNA量を用いた逆転写用に特別にデザインされています。 RNAへのアフィニティーが高いOmniscript Reverse Transcriptaseは、他の逆転写酵素と比較して非常に優れた性能を示し、低いコピー数の. The assays were run under the following conditions. Unit definition: 2 Products found Omniscript RT Kit For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Sensiscript RT Kit For reverse transcription of less than 50 ng RNA per reaction for end-point PCR Discover the products and protocols you need Plan your entire experiment based on research interests. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript®andSensiscript®RT WiderangeofRNAamounts(1pg–2µg) Highsensitivity OneStepRT-PCRBuffer Minimaloptimizationneeded. アフィニティーが高い酵素による高収量cDNA わずか10コピーのテンプレートも高感度で検出 追加のRNase H分解は不要 煩わしいピペッティング操作なしの迅速で簡単な製法 Omniscript Reverse Transcription（RT）Kitは、各反応あたり50 ng. Omniscript is a confident hand-lettered script without self-conscious style or idiosyncrasy. There-fore, 2,500 copies (1 fg) was chosen as the relatively small. Investor Relations Contacts. この例には、再利用可能な. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR(RT-PCR) RNA was extracted from cultured macrophages with RNeasy Mini Kit (Qiagen, Hilden, Germany) and made into cDNA with Omniscript RT Kit (Qiagen) according to the manufac-turer’s instructions. Cycling conditions consisted of a single denaturing step at 94C for 15 min followed by 33. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Workflow for Configuring OmniAnalytics for a Third-Party Analytics. Pharmaceutical Industry. In a final. Time step T0 contained the reverse transcription mix (Omniscript RT Kit) without performing reverse transcription, T1 after the reverse transcription, and T2 was after a multiple displacement. ers and the same RT kit. show that ferrets are highly susceptible to SARS-CoV-2 infection and effectively transmit the virus by direct or indirect contact, recapitulating human infection. Two micrograms of total RNA was reverse-transcribed using an Omniscript Reverse Transcriptase Kit (Qiagen) in a total volume of 20 µl. Reverse Transcription Kit (Omniscript® RT Kit) as per the manufacturer's instructions (Qiagen). 4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. Your reverse transcription may not be optimal. . RT-PCR Assays. Kit contents: Qiagen Omniscript RT Kit, 50 rxns, RNA Template Sample, Reverse Transcription Enzyme Activity, High cDNA Yields Due to High-affinity Enzyme, Fast and Easy Procedure, For Reverse Transcription of 50ng to 2micro g. 1 ml RNase-free water 205111 询价For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. The Omniscript RT Kit is intended for molecular biology applications. PrimeScript RT Reagent Kit is designed to perform reverse transcription optimized for real-time RT-PCR. The kit makes fast, efficient cDNA template synthesis for real-time PCR possible. Direct quantification of six different targets in human total RNA using GoTaq® 2-Step RT-qPCR System. Format. , Valencia, CA). Differences Between OmniStudio and OmniStudio for Vlocity. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. . For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Enzyme activity: Reverse tran…The Sensiscript Reverse Transcriptase (RT) Kit is ideally suited for highly sensitive applications using very small amounts of RNA (less than 50. A total of 1. 00. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Need Assistance?. First-strand cDNA synthesis was done using the Omniscript RT kit (Qiagen, Valencia, CA) as recommended by the manufacturer. The Agilent 2100 Bioanalyzer (Agilent Technologies; Kista, Sweden) was used to confirm the integrity of the RNA. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Commercial Partner and Distributor Solutions. Bioz Stars score: 86/100, based on 1 PubMed citations. , 2018). For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. An OmniScript is an omnichannel customer engagement and a business tool built on the Salesforce platform. At higher temperatures, RNA secondary structures unravel and no longer. 1 mL RNase-free water. Mix carefully by vortexing for no more than 5 s, and centrifuge briefly to collect residual liquid from the sides of the tube. EGFP cDNA were obtained with th e other RTases. Short cycling steps withoutExplore targets and avenues in their scientific context, find and adjusting products till study i, analyze details and plan follow-up studies – all in GeneGlobe. 1 ml 1. File size. OmniScript にはモジュール形式のアーキテクチャが採用されている OmniScript の外観 (フロントエンド) は機能 (バックエンド) から切り離されています。 OmniScript では JavaScript Object Notation (JSON) メタデータ構造 (1)、スタイルシート (2)、データ (3) が互いに分離され. CNV qPCR Assays & Arrays;. 4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. Differences Between OmniStudio and OmniStudio for Vlocity. Omniscript RT Kit. 1 mL RNase-free. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. Kit contents: Qiagen Omniscript RT Kit, 50 rxns,. Starting material Starting material QIAGEN now offers the first commercially available reverse transcriptases from a new source. Kit contents: Qiagen Omniscript RT Kit, 200 rxns, RNA Template Sample, Reverse Transcription Enzyme Activity, High cDNA Yields Due to High-affinity Enzyme, Fast and Easy Procedure, For Reverse Transcription of 50ng to 2micro g. , GC-rich. Actin and CD30 cDNA transcripts were quantified using AB&I TaqMan primer and probe sets (Life Technologies) on a Bio-Rad CFX96 real-time. 1 ml 4 x. Note: Commercially available RNase inhibitor is commonly supplied at 40 units/µl. (2013) NA mRNA invA-R García et al. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniScripts. Reverse transcription (RT) is a critical step in a real-time PCR. The cDNA was generated using the Omniscript RT kit (Qiagen) with random hexamers (Roche Products, United States). For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. Let’s take an example of two products — DSL Service and DSL Modem. 1. Add 2 μl of Buffer RT (component of Omniscript RT Kit), 2 μl of dNTP Mix (component of. , the OmniScript RT, an RNAse H+ RT, which is part of the QuantiTect Reverse Transcription Kit, as well as with RNase H. # 18064 and 18080, respectively), and Omniscript® and Sensiscript® (Qiagen Cat. IVDR Support. Relative quantification of mRNA levels by RT-qPCR was performed using a SYBR Green PCR kit (Qiagen Inc. First-strand cDNA was synthesized from 1 μg of high-quality total RNA using SuperScript II Reverse Transcriptase in accordance with the manufacturer’s instructions (QIAGEN, Omniscript RT). The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription from 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRSensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). QIAGEN now offers the first commercially available reverse transcriptases from a new source. OmniScript を使用しましょう。. When the reaction is heated to 50°C, the RT-blocker dissociates from the RT enzymes, rendering them fully active. 0 µl 5 mM dNTP (final concentration of 500 µM), 2. DSL Modem has an attribute Grade (ATT_RT_GR) and possible values are Good, Better and Best. Time step T0 contained the reverse transcription mix (Omniscript RT Kit) without performing reverse transcription, T1 after the reverse transcription, and T2 was after a multiple displacement. Technical Service; Customer Care . For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRReverse transcription was carried out according to the manufacturer's protocol either with the Omniscript RT (Qiagen) or SuperScript II RT (Invitrogen). High Ca2+ stimulated the upregulation (as assessed by RT-PCR) and the secretion of Wnt5a (assessed by Western blot), a. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. After reverse transcription by Omniscript and Sensiscript Reverse Transcriptases, reactions are heated to 95°C for 15 minutes to activate HotStarTaq DNA Polymerase and toFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. Omniscript RT Kit (200) by Qiagen. It uses PrimeScript Reverse Transcriptase, which features excellent extension. (B) Plasmid map of the predicted Omniscript RT Kit expression plasmid which was identified as the source of the EIAV pol. OmniStudio Standard. ). 00. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µL 10 × Buffer RT, 100 µL dNTP Mix (contains 5 mM each dNTP), 1. Omniscript RT Kit. It relies on the principle of reverse transcription which is a process ‘reverse’ to the transcription. Bioz Stars score: 86/100, based on 1 PubMed citations. activatedtooearly reverse-transcription reaction is complete (30 min at 50°C) before activating the HotStarTaqDNAPolymerase(15minat95°C). Product tracking (in bp) are shown about the right of the panel (lane 9). First Strand kit)? What is recommended? We have had a good experience with both types of Reverse Transcriptases, e. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapidly spreads, leading to a pandemic infection. 1 ml 1. Probably the most important being that the OmniScript RT allows for the labeling of even very small amounts of RNA (i. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. , SuperScript vs. Reverse transcriptase is a multifunctional enzyme with three distinct enzymatic. Total RNA from flag leaves of mature rice plants was isolated using the RNeasy Plant Mini Kit (Qiagen, Venlo, The Netherlands) and reversely transcribed using Omniscript RT Kit (Qiagen). This kit's protocol is simple and suitable for high throughput. AIV genomes were amplified from extracted RNA using universal and gene-specific primers with an Omniscript reverse-transcription kit (QIAGEN, Germantown,. A Vlocity OmniScript guides users through complex processes with fast, personalized, and consistent responses. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRSensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). This kit is ideal for analyzing low input RNA samples. 1 ml 1. Bioz Stars score: 86/100, based on 1 PubMed citations. The QIAGEN One-Step Ahead RT-PCR Kit contains a blend of Sensiscript and Omniscript Reverse Transcriptases, a well-balanced combination of Taq DNA Polymerases and a proofreading enzyme. RT reactions containing 4 ng (Sensiscript) or 16 ng of total RNA (Omniscript) generated DD patterns indistinguishable from the 200 ng pattern. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRRT reactions containing 4 ng (Sensiscript) or 16 ng of total RNA (Omniscript) generated DD patterns indistinguishable from the 200 ng pattern. All seven samples were reverse transcribed by the same kit toFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. For reverse transcription of 50 ng to 2 micro g RNA per reaction for end-point PCR. 05). Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. The commercial RT systems tested were Sensiscript, Omniscript, SuperScript II, SuperScript III, and PowerScript. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. For standard reverse transcription, with 50 ng to 2 µg of RNA per reaction, Omniscript RT provides optimal results for both high- and low-copy mRNA species. Global contacts. 1 ml RNase-free water. This enzyme combination provides highly efficient and sensitive reverse transcription over a wide range of RNA templateLightning Experience. A high affinity for RNA allows Omniscript Reverse. Omniscript Reverse Transcriptase is a new, unique enzyme, different from the reverse transcriptases of Moloney murine leukemia virus (MMLV) or avian myeloblastosis virus. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Omniscript is designed for reverseFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThis study provides a complex overview of the influence of elements such as RT systems, qRTPCR chemistry, amount of background RNA, and transcript abundance on the efficiency of qRT-PCR. This kit is ideal for analyzing low input RNA samples. In reverse transcription, the cDNA is synthesized from the mRNA. EGFP cDNA were obtained with th e other RTases. was reverse transcribed to cDNA using the Omniscript RT kit (Qiagen) and random hexamer primers (Invitrogen; Ta˚strup, Denmark). Sensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). . Reverse Transcription Troubleshooting Guide. , M-MuLV or AMV), and the kind of approach used for RT (random hexamers, oligo-dT, or specific. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. 2) If you only wanted to produce cDNA from a subset of all mRNA, then. Omniscript RT system, whereas substantial copies of. The QIAGEN One-Step RT-PCR Kit provides a blend of Sensiscript and Omniscript Reverse Transcriptases, HotStarTaq DNA Polymerase, QIAGEN OneStep RT-PCR. High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. Genomic DNA contamination in RNA samples is effectively. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. 4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. 1 ml 1. DSL Service has an attribute Download Spee d (ATT_RT_DS) and possible values are 7, 12, 20 and 40 Mbps. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRQuantiTect RT Mix contains an optimized Omniscript and Sensiscript blend. Total RNA was isolated from drug-treated cells with TRIzol (Invitrogen) and then reverse-transcribed to cDNA using the Omniscript RT kit (Qiagen, Valencia, CA). For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript Quantiscript 37 NA mRNA Random hexamers Paulin et al. For MMLV Superscript, the pattern obtained was very faint at 200 ng of RNA, but indicated a >50-fold increase in detection levels for Sensiscript. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor standard reverse transcription, with 50 ng to 2 µg of RNA per reaction, Omniscript RT provides optimal results for both high- and low-copy mRNA species. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. Two micrograms of total RNA was reverse-transcribed using Omniscript RT kit (Qiagen). High yields of cDNA 특이성을 높인 참신한 enzyme과 buffer No additional RNase H digestion step required Robust system guarantees consistent results The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRQiagen omniscript rt kit Omniscript Rt Kit, supplied by Qiagen, used in various techniques. これにより、特定の取引先責任者や取引先の項目を表示するなどの一般的な機能を持つ小さな「子」 OmniScript を作成することができます。. Each experimental condition was run in 10 RT replicates unless stated otherwise. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Omniscript RT Kit. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Product Description. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. The RNA yield and quality were determined using a NanoDrop system (ND-1000; Thermo Fisher Scientific). However, RT is recognized as a known source of variability, particularly with low amounts of RNA. OmniScript にはモジュール形式のアーキテクチャが採用されている OmniScript の外観 (フロントエンド) は機能 (バックエンド) から切り離されています。 OmniScript では JavaScript Object Notation (JSON) メタデータ構造 (1)、スタイルシート (2)、データ (3) が互いに分離され. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. The easy one-tube setup and optimized components enable higher sensitivity. Omniscript Reverse Transcriptase - For First-strand cDNA synthesis Two-tube RT-PCR. Mar 15, 2021. using an Omniscript reverse transcriptase kit (Qiagen, Valencia, CA) in a 20- l reaction mixture, containing 200 ng of total RNA and specific primers, at 37°C for 1 h. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFAQ. Migrate to Standard OmniStudio. 1 ml 1. Omniscript RT Kit. It is also nice that the purification procedure (after labeling) is simple. DSL Modem Product. The unique QuantiTect Reverse Transcription Kit provides a fast and convenient procedure for cDNA synthesis with integrated genomic DNA removal. CNV qPCR Assays & Arrays;. QIAstock. Kit contents: Qiagen Omniscript RT Kit, 200 rxns,. High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. 1 ml 1. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript Reverse Transcription Handbook 05/2004. The purification steps can be performed as described in the protocol using Qiagen’s PCR purification kit. Content. RT-qPCR was carried out inRNA concentration was photometrically determined, and 200 ng RNA was reverse transcribed using Omniscript RT kit with oligo dT Primer (Qiagen, Hilden, Germany). My QIAGEN. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. CNV qPCR Assays & Arrays;. Migrate to Standard OmniStudio. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Key words: Breast cancer, Formalin-fixed, Paraffin-embedded tissue, Quantitative RT-PCR,Reverse Transcription was performed using an OmniScript™ RT kit (QIAGEN) according to manufacturer’s instructions and using gene-specific primers to interrogate genes of interest. SYBR Green (DyNAmo SYBR Green qPCR Kit; Finnzymes, Finland) and related accessories to perform real-time RT-qPCR (Opticon2; MJ Research, Watertown,. Subsequent PCR was performed with primers (Sigma-Aldrich) based on the Icelandic rock ptarmigan genome and Taq DNA polymerase (QIAGEN). Omniscript and Sensiscript QuantiTect RT Mix contains an optimized Omniscript and Sensiscript blend. Bioz Stars score: 96/100, based on 1 PubMed citations. Kim et al. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Please see the Omniscript Reverse Transcription Handbook for protocols. CNV qPCR Assays & Arrays;. Qiagen omniscript reverse transcription rt kit Omniscript Reverse Transcription Rt Kit, supplied by Qiagen, used in various techniques. 4 Omniscript Reverse Transcriptase Handbook 04/99 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog No. The commercial RT systems tested were Sensiscript, Omniscript, SuperScript II, SuperScript III, and PowerScript. For MMLV Superscript, the pattern obtained was very faint at 200 ng of RNA, but indicated a >50-fold increase in detection levels for Sensiscript. Specific genes have been used as baseline controls to. Reverse transcription yields could vary among the reverse transcriptases in a highly gene-dependent way as a. Media Inquiries. Isolation and cloning of random PCR products. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. 95°C after the reverse transcription step. A PCR was conducted using standard PCR methods (primers are available on request). Global Contacts. , 2015:Omniscript Reverse Transcription Kit Quick-Start Protocol (EN) - QIAGEN. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. 1 ml RNase-free water 205111 询价Currently, almost all single-cell transcriptomic protocols rely on reverse transcription (RT). The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. It is a quantitative technique used to estimate gene copies. At this reaction temperature, common restrictions of RT-PCR are removed, simplifying assay design and eliminating compromises. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. QIAsphere Digital Connectivity. Additional Information. For 200 reverse-transcription reactions: 800 units Omniscript Reverse Transcriptase, 4 × 150 µL 10 × Buffer RT, 4 × 100 µL dNTP Mix (contains 5 mM each dNTP), 4 × 1. Nine parallel large-scale GoScript™ RT reactions were performed, containing log-fold variations of input human reference total RNA (10ng/µl to 0. g. coli. High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript Reverse Transcription Kit Quick-Start Protocol (EN) Print Bookmark Share pdf 56KB English Format File size Language Download Get Adobe Reader Contact QIAGEN . Reverse transcription with AMV, MMLV, and Omniscript was performed at 37 °C, whereas with cAMV, Improm-II, and MMLVH it was performed at 45 °C, and with ThermoScript and SuperScript, it was performed at 50 °C. Reactions containing 2. g. Both. Total RNA (150 ng) was used for cDNA synthesis using the Omniscript RT Kit (Qiagen). Business Development. 1 ml RNase-free water 205111 询价 The Omniscript RT Kit includes Omniscript Reverse Transcriptase, dNTPs and an optimized reaction buffer. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript® Reverse TranscriptionFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR3 min read. Omniscript RT Kit. ZERO BIAS - scores, article reviews, protocol. In order to measure this difference on a large spectrum of background RNA, given that DNA and RNA concentrations are important influencing factors [ 8 – 10 ], the study used total RNA extracted from the same testis tissue (Methods). Please see the Omniscript Reverse Transcription Handbook for protocols. 0 µl of Omniscript or Sensiscript RT (Qiagen, Santa Clarita, CA), 2. Sample to Insight__ Quick-Start Protocol March 2016 Omniscript® Reverse Transcription Kit The Omniscript RT Kit (cat. Components. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. . The maximum amount of viral RNA was used in place of water for both reactions. For standard reverse transcription, with 50 ng to 2 µg of RNA per reaction, Omniscript RT provides optimal results for both high- and low-copy mRNA species. ) to obtain cDNA. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. For example, you can create an OmniScript t. 0 µl of 10 µM H-T11C (final concentration of. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. It allows for the efficient labeling of a wide range of starting RNA concentrations and increased the signal of our microarrays by 2-5 fold. 1 ml RNase-free water 205111 询价 Reverse transcription and quantitative real-time PCR (RT-qPCR). An OmniScript is an omnichannel customer engagement and a business tool built on the Salesforce platform. For one-step RT-PCR, the optimized Omniscript and Sensiscript blend for the reverse-transcription step further enhances sensitivity. SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. To minimize methodological errors resulting from. CNV qPCR Assays & Arrays;. Kit contents: Qiagen Omniscript RT Kit, 50 rxns, RNA Template Sample, Reverse Transcription Enzyme Activity, High cDNA Yields Due to High-affinity Enzyme, Fast and Easy Procedure, For Reverse Transcription of 50ng to 2micro g RNA/Reaction for end-point PCR, Includes 200U Omniscript Reverse. OmniStudio Standard. Here, we pres-ent an updated description of current best practices for isolating both mRNA and microRNA from FPE tissues for RT-PCR-based expression profiling. The ABScript II Reverse Transcriptase in. PCR was performed with HotStarTaq PCR Kit (Qiagen). Please see the Omniscript Reverse Transcription Handbook for protocols. RNA, using the Omniscript RT kit (Qiagen Inc. This kit's protocol is simple and suitable for high throughput. High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. The mRNA levels were quantified using a SYBR Premix Ex Kit (Bio‑Rad) with a CFX Real time PCR Detection Systems (Bio‑Rad). 1 ml 4. It uses PrimeScript Reverse Transcriptase, which features excellent extension. 5 mM each dNTP, 10 U of RNase inhibitor, 4 U of Omniscript Reverse Transcriptase, and. nos. Quantitative real-time PCR (qPCR) was performed using the SYBR Premix Ex Taq (TaKaRa, Dalian, China) and the CFX96 TM Real-Time PCR. RT-PCR Analysis . 3. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRLaunching an OmniScript with an OS Action in a Community; Launching an OmniScript from a Layout Action; Launching an OmniScript with an OS Action in Lightning; Launching an LWC OmniScript in Lightning; Launching an OmniScript in Lightning; Launching an OmniScript From the Action Toolbar; Launching an LWC OmniScript from an OS. Marketplace Solutions. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. ZERO BIAS - scores, article reviews, protocol conditions and more For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. The PCR products were resolved by electrophoresis on 1% agarose gels and visualized by ethidium bromide staining. RT-PCR; PCR for Difficult Targets; Fast PCR; Long PCR; Direct PCR; High Fidelity PCR; Hot Start PCR; Real-Time PCR; Routine PCR ReagentOmniscript Rt Kit, supplied by Qiagen, used in various techniques. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRExamine OmniScript Elements. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. 次の画像で. The QIAGEN OneStep RT-PCR Enzyme Mix contains a specially formulated enzyme blend for both reverse transcription and PCR. Serial analysis of BCR-ABL mRNA levels by real-time quantitative PCR (RQ-PCR) accurately reflects the level of leukemic inhibition induced by therapy and provides. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRReverse transcription is described based on the Omniscript ® Reverse Transcription Kit (Qiagen). The forward primer (5′ to 3′) GAAGCCGCTTGGAATAAGGC and the reverse primer (5′ to 3. reverse transcription, and quantitative PCR to work optimally in archived clinical samples. However, the Sensiscript and PowerScript systems were more efficient for detecting high. For example, performance of OmniScript and SensiScript (both Qiagen) or AMV-derived RTases have been heavily influenced by experimental. nos. The sequences of the primers used in. 0 μg of RNA was reverse-transcribed using the Omniscript RT Kit (Qiagen Inc. 1 ml 4. Tie a Picklist to Salesforce. 205110, 205111and 205113) should be stored immediately upon receipt at –30 to –15°C in a constant-temperature freezer. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. The Sensiscript Reverse Transcriptase (RT) Kit is ideally suited for highly sensitive applications using very small amounts of RNA (less than 50 ng), such as single-cell RT-PCR and analysis of biopsies and LMD samples. However, it is extremely difficult to maintain linearity across the entire procedure, especially at the step of PCR amplification. After the cDNA was diluted 1:3 in nuclease-free water, qRT-PCR was performed using the custom-designed primers (Biomers, Ulm, Germany) in Table 1 and the Platinum SYBR Green qPCR SuperMix-UDG kit (Invitrogen), or TaqMan Gene Expression Assays and. Four weights together with monospaced numerals and math symbols make it ideal for. The QIAGEN OneStep Ahead RT-PCR Kit is designed for easy and sensitive one-step RT-PCR using any RNA template. Data Migration with Build Tool. 47 / EA. One microliter of kit-supplied enzyme mix-ture (combination of Omniscript Reverse. Omniscript reverse transcriptase (QIAGEN) using random hexamers and oligo dT and. Martin Bilban, MSc PhD Student Quaranta Lab Department of Cell Biology The Scripps Research Institute The Omniscript RT Kit includes Omniscript Reverse Transcriptase, dNTPs and an optimized reaction buffer. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. g. The ABScript II Reverse Transcriptase in the. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRSensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). OmniScripts 要素 (子 OmniScript) は、OmniScript をコールして別の OmniScript 内で再利用します。. PCR was performed on an ABI 7900HT using TaqMan® Universal PCR Master Mix, No Amperase® UNG (Life Technologies, Carlsbad, CA) or. Popular answers (1) "If the mRNA has a poly-A 3' tail, then an oligo-dT primer can be used to prime all mRNAs simultaneously. The unique combination of Omniscript and Sensiscript Reverse Transcriptases, with their high affinity for RNA templates, ensures highly efficient and. OmniStudio. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. CNV qPCR Assays & Arrays;. For each RTase, the reaction conditions and thermal profile followed the recommended protocol issued by the manufacturer (see Protocols in online Data Supplement 1). CNV qPCR Assays & Arrays;. . 1 ml 4. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. Related products . 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1.