Published Dec 16, 2020 | Updated Dec 16, 2020. Lower limits of detection for ATP determined using the ATPliteAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. 2. . For readers without a cuvette port, simply check the box next to ‘PathCheck’. Step 1: Open the SoftMax Pro IMAP FP protocol. Please refer to the Obsolete Microplate Reader Models for more information. Microplate Readers BioTek Epoch 2 Microplate Reader. zip. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. A microplate reader as The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Detailed specifications of each model reader are reported in Section 7, “Specifications”. With optimized reagents, option to operate the. Reader modes are customized to your specific applications with options to upgrade easily. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Selection of Water Constant in PathCheck settings. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Figure 2-23: Bottle holder To install the bottle holder: 1. Order Now. e. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. 2. The plate reader optics are tunable for absorbance. SpectraMax Mini User Guide. limit of detection (LLD) for ATP was estimated for each microplate reader using the . unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. When the user applies a set temperature of 37°C, the top is heated to 37. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. 1 software Download page. We would like to show you a description here but the site won’t allow us. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Examples: “Press carefully to engage,” “ Do not. Prerequisites: SoftMax Pro 7. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 2 only) SpectraMax M-series Microplate readers. The dual monochromator optics allow. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. 314 low lamp level warning. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Offer Ends In: 2 Days 10: 40: 19. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. The screw remains inside the instrument. Other technologies such as imaging. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. The plate. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. SpectraMax Glo Steady-Luc Reporter Assay Kit. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Absorbance (optical density, OD) was read at 570 nm and 605. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). 33 Dramatic aggregation of Alzheimer A b by Cu(II) is induced by conditions representing physiological acidosis . The advanced optical and. It also offers a field-upgradeable cellular imaging option and a cooled PMT for low-light sensitivity. We would like to show you a description here but the site won’t allow us. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. We would like to show you a description here but the site won’t allow us. Access this by going to Help >. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. . 315 can't find zero order. Three low volume 24-well bottom slides. Category 2 and 3 microplate readers use gradient heating as well. 2. Microplate readers’ standard detection modes. Using the optimized wavelength settings, the SPECTRAmax GEMINI XS fluorescence microplate reader can distinguish between live and dead cells using this assay. SoftMax® Pro 7. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile microplate reader capable of quantitating samples in a variety of microplate formats. Figure 1. . The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. . The system uses two scanning monochromators to determine the optimal excitation and emission settings. View the Molecular Devices website. ¨0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingThe SpectraMax iD5 Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Summary of Contents for Molecular Devices SpectraMax M2. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 6% for the on-board optics (monochromators). For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. western blot membrane detection in a multimode microplate reader platform. The calculated LOQ values were 0. Download PDF versions of the SpectraMax Microplate Reader User Guides. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The SpectraMax 190 reader can be integrated with Molecular . . The powerful analysis tools in the software generate a standard curve and calculated data. Promega's ROS-Glo H202 assay can be combined with SpectraMax readers to explore the relationship between. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Data exemplifying linear ranges from the three different kits are presented in Figure 2. The drawing shows a standard microplate of 15 mm height covered with a lid. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Italic and boldface type are used for emphasis. 58% for the detection module and 2. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual. We would like to show you a description here but the site won’t allow us. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. This unlocks the transport slide. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). com 4. Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. Use similar settings for other Molecular Devices readers with fluorescence detection mode. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Plate chamber design of the SpectraMax category 1 microplate readers. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Highly customizable low volume. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Time-. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Figure 6: Transcreener ADP2 FI assay principle. microplate reader. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. 5 12. Minimize manual manipulation of samples and data processing, and improve laboratory efficiency. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Five preferred detection modes. Lift up the lid over the chamber to insert or remove a cuvette. both cuvette-based and microplate reader applications Microplate Readers. Our SpectraTest® ABS2, FL1, LM1, SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. SpectraMax 250 User. Cell Analysis. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. Guides: SpectraMax Mini Installation Guide. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both superior. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. The advanced optical and. Self Service Available. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. These lab workhorses are used in drug discovery. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. Temperature control and shaking come as standard. The dual monochromator optics allow the. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 306 plate air cal. keeping with best practices, reader performance should. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. Wide range of applications The VersaMax reader covers. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. For a higher . Example: [Drawer]. Download the SpectraMax Mini Reader Plug-in and associated guides. SpectraMax Microplate Readers. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. For the calculation of the ratiometric biosensor signal, the emission intensity at 510 nm was recorded upon an excitation at 380 nm and 470 nm and the former was divided by the latter (Exc. Table 1. Lysozyme contains two tryptophan residues that. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. This pathlength correction is accomplished only with the use of. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Endpoint, kinetic, spectrum. Fully tested with validations, warranty. Maximum signal with lowest background. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Page 28 Chapter 5: Read Modes and Read Types 1. Find regional support. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Unparalleled performance on a personalized platform. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. In addition, the SpectraMax iD5 can be expanded to. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Using the. flexibility of monochromator-based systems. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. 306 plate air cal fail,low light. 1. Path Check Pathlength Measurement SpectraMax i3x. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMinimum volume and maximum throughput with 96- or 384-well microplate compatibility. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Here, we demonstrate performance of the SpectraMax® iD3 Multi-Mode Microplate Reader for assays measuring intrinsic tryptophan fluorescence. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. 0 out of 5 $ 5,999. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. The plate reader optics are tunable for. Add to Cart. Thank you!. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. Endpoint, kinetic, spectrum. Measuring platelet aggregation with a microplate reader. 030 fmol Fluorescence Polarization (FP). Microplate Spectrophotometers. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Type:Spectrophotometer. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. In . 7°C (gradient heating design). Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. We would like to show you a description here but the site won’t allow us. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. If it looks fine, then proceed. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. SpectraMax Microplate Readers. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. Temperature control. Figure 1: Endotoxin standard curves on SpectraMax microplate readers, plotted using a log-log curve fit. Our absorbance plate readers feature our PathCheck Sensor technology and. Sale. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. SpectraMax® Mini Multi-Mode Microplate Reader for Biotech Laboratories Built on the company’s nearly four decades of experience delivering high-performance microplate reader technology to the market, the SpectraMax Mini reader is robust enough to meet a lab’s core microplate reader needs, while its compact size is ideal for integration into a. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. We would like to show you a description here but the site won’t allow us. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. Cuvette Microplate wells. Microplate automation and detection instruments for walk away operation and high quality results. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Offer Ends In: 18 Days 19: 38: 21. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Find regional support. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Learn more about its features, specifications, and applications in this user. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. IMAP FP Settings in SoftMax ProSpectraMax M5 Multi-Mode Microplate Reader. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. The calculated LOQ values were 0. The 2-channel configuration is available with 0, 2, or 4 injectors. SpectraMax M5 reader settings are shown. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. . The temperature in the plate chamber is ambient and isothermal. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. For more sample throughput, the system can SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Step 4: Incubate at room temperature for 1 hour, protected from light. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 72 MB. 305 reference level saturation. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). In addition, the SpectraMax iD5Next generation SpectraMax® absorbance microplate readers. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. Table of Contents. , a. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Each sample has a discrete sample beam and reference beam so that each well is measured directly. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. More Product Information. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. For readers without a cuvette port, these options are not shown in the software. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Total volume (except deep. The CellTiter-Glo assay from Promega uses luciferase. The SpectraMax microplate readers can be used to accurately quantify ROS levels using a luminescence-based assay. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Read one sample or up to 384 inThe SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Materials. 0 out of 5 $ 5,999. Devices’ StakMax® Microplate Stacker in a matter of minutes and . The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. 380 nm/Exc. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax i3x. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. Devices’ StakMax® Microplate Stacker in a matter of minutes and . It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Choose from a three- (M3), four- (M4), orIn this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Or scan up to 384 wells at any time to determine the best assay conditions. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget.