LbL films are immersed into 20 mM DTT in PBS buffer (2. The same procedure. ). The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Eis inhibitors were dissolved in Tris-HCl (50 mM, pH 8. Fluorescence, luminescence, and UV-Vis absorbance multimode plate reading. For the Evans blue (EB) dye extravasation test, 3,8. SpectraMax Injector Cartridge with SmartInject™ Technology. 2 only) SpectraMax M-series Microplate readers. 2 mg/mL using 2 different UV-visible spectrophotometers, a SpectraMax M5 plate reader, and a NanoDrop instrument. S. FeCl 3, iron standards are always included (0-4 µg /ml). An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Cell Titer Glo reagent (Promega) was then added to each well, and luminescence (RLU) was measured on a SpectraMax M5 plate reader (Molecular Devices). The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Plate geometry 6- to 384-well plates, Petri dishes Dispense precision ≤2% at 50-200 μL Dispense accuracy ±1 μL or 2% Physical Characteristics Power 250 Watts max. The following document is. Flock Safety is an Atlanta-based company that sells automatic license plate detection readers, or ALPRs for short, to law enforcement agencies, schools, neighborhood homeowners associations and. SpectraMax Paradigm Multi-Mode Safety Guide fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Microplate Readers; SoftMax Pro Software; Microplate Stacker and Washer; Cellular Imaging Systems; Clone Screening; FLIPR; AXON Patch-Clamp; Assay Kits. Accordingly there are different configurations possible. Certain functionalities in the table above requires specific configurations. 14. The SpectraMax 190 reader can be integrated with Molecular . With SOFTmax PRO, the contents of the wells in a microplate can be mixedAdjusting fluorescence gain on microplate readers. Published Dec 16, 2020 | Updated Dec 16, 2020. Purified fluorescent protein was diluted 15-fold into 0. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. SpectraMax M5 plate reader (Molecular Devices, San Jose, CA) was used. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 1 M NaCl at varying pH levels (3–10) and incubated at room temperature for 1 h. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Five preferred detection modes. I’m. 40266 Email: drugdiscoverytech@invitrogen. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Standards and QCs are composed of a. 5. The concentration of infectious virus in both stock. This is a result of the light being attenuated and scattered by the. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. SpectraTest ABS2 Absorbance Validation Package performance tests. To monitor the aggregation kinetics, 10 μM ThT was added to a small aliquot (300 μL) of the mixture, and the fluorescence signal (λ ex = 444 nm, λ em = 485 nm) was monitored on a Spectramax M5 plate reader (Molecular Devices). Molecular Devices M5 plate readers; Multiple liquid bulk dispensers (Wellmate, Multidrop Combi) Price List. How to export results files from the plate reader into Excel. ScanLater Western Blot Detection Cartridge. The EPOCH2 can easily develop various types of assays (wavelength scan: 200–999 nm), and its temperature control (up to 65 c) is excellent. Developed with Promega reagents to provide a. 0 μM wild-type R3/I5. 18632/oncotarget. The SpectraMax® ABS Plus Microplate Reader can run . Multimode Readers Comparison Chart. 5 h (during the lag phase), 7 h (during the exponential phase), and 24 h (during the plateau phase) for analysis of the fermentation. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only (blank) and four wells containing 50 nM ATP (sample). cerevisiae transcription factor Azf1p Taylor Stewart1, Benjamin E. 6. The colorimetric reaction was stopped with 2 M sulfuric acid, and absorbance was measured at 450 nm using a SpectraMax M5 plate reader (Molecular Devices). Table of Contents. Catalog number: 165306. 5 pM (0. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. ". The multi-mode. After the plate is read, data will appear in the plate section: 11. Microplate Reader Shelf. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Description Figure 1. 5 hours after an initial 10-minute incubation period. Molecular Devices. Microplate Readers Fisher Scientific AccuSkan FC Microplate Reader – 2020. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. 0200-0729. Wide range of applications. Help the planet as you cruise along at 5X speed. Absorbance was measured at 405nm on a Molecular Devices SpectraMax M5 plate reader. reader (Molecular Devices). The TRITC and FITC labeling process is conducted as in a previous study . plate and push the “drawer” button (this does not need to be done before opening/exploring the software, but must be done before reading the plate, of course). Results: In the presence of Cliradex ®, Johnson’s® Baby Shampoo, OCuSOFT Lid Scrub®were read using SpectraMax M5 plate reader (Molecular Devices, San Jose, CA, USA) with excitation of 380 nm and emission of 460 nm. 1 or 10 Tablet PC with USB connection to instrument. Table of Contents. Cell Analysis. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86After 5 minutes of incubation at room temperature, luciferase activity was assessed by SpectraMax M5 plate reader (Molecular Probes). 2016 Aug 2; 7(31): 50027–50042. Absorbance. 1. 5. 17. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 2–200 pg/well). Published online 2016 Jul 13. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 51 L/g). 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. -Glo assay solution (Promega) at room temperature and luminescence measured at 1000 ms −1 integration on a SpectroMax M5. Wolfe1, and Stephen M. 3. The eight mammalian Src-family tyrosine kinases are dynamic, multi-domain structures, which adopt distinct “open” and “closed” conformations. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Endothelial permeability was also measured in vitro, using a transwell assay. Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readers Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Transfection of siRNA into cells. When the user applies a set temperature of 37°C, the top is heated to 37. ameters by UV-Vis assays was done on a multimode SpectraMax M5 plate reader by using 96-well plates (Fisher Scientific). Supernatants were collected, and the concentration of l-lactate was determined by measuring absorbance of reaction samples using the Spectra Max M5 plate reader (Molecular Devices). If the computer is connected to the Internet: Select Search Automatically for updated driver software. Uncontrolled Copy for Training and Reference Purposes Only . The data analysis was performed by GraphPad Prism. Small molecule ligands that engage the regulatory. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Plate chamber design of the SpectraMax category 1 microplate readers. to meet all your research needs. 2. LbL films containing TRITC- or FITC-labeled PAA are degraded by DTT and monitored by a fluorometer (SpectraMax M5 Plate Reader, Molecular Devices). Supersedes 1. The medium fluid was, respectively, collected at 4. 40266 Email: [email protected] fluorescence intensity was determined using a fluorescent plate reader (SpectraMax M5 plate reader) set at an excitation wavelength of 485 nm and an emission wavelength of 530 nm. ScanLater Western Blot Detection Cartridge. 2013): the specific activity of both enzymes was ~1. This is a result of the light being attenuated and scattered by the. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Options include top and bottom detection, temperature control and reagent injector. Please contact Tatiana to reserve the plate reader time: tnl1@psu. 9. 1. Read More. PDE-lux transcriptional fusion plasmids were constructed as previously described. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. S. Multimode Reader Comparison Chart. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. , 2018b). The EC50 values were calculated from the absorbance values vs. Each microtiter plate was sampled three times on different days, and each of the recipient plates were separately assayed with a BioTek Synergy2 microplate reader. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. For a Price List, please call (858) 646-3100 ext. Table of Contents. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Quantitative PCR RNA was collected in duplicate from 1 × 10 6 cells lysed in TRIzol Reagent (Invitrogen, 15596018) after. 11. The emission of roGFP2 was measured between 500 and 530 nm after excitation at 405 nm and 488 nm every 30 s in a SpectraMax M5 plate reader (Molecular Devices). Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. The percentage of inhibition was calculated using the following formula: O D s R A G E - O D s a m p l e O D s R A G E × 100 ( % ) where OD sRAGE was the signal obtained for sRAGE incubated without the. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. The amount of HRP encapsulated in liposome was calculated from the calibration curve of. SpectraMax M5 Plate Reader: 901F: Nanodrop 2001: BioRad ChemiDoc gel documentation (old) Bio-Rad T100Tm Thermal Cycler : Bio-Rad CFX ConnectTM Real-Time System : Location. The inhibitory rate of ingredients was calculated as follows: Inhibition (%) = (FI of control – FI of treatment) / FI of control × 100. 0 . edu, 814-863-3503. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. To the OP, if you are familiar with VAG-COM, you know that RS232-based VAG-COM cables are in the $14 range on eBay. We use Spectramax M5 microplate reader in our lab. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. The password is. In the M2, M5 and Plus 384, the light. 1 software to identify the IC50. Fluorescence, brightfield, color brightfield, and phase contrast imaging are available with the automated cell imager. 6-, 12-, 24-, 48-, 96- and 384-well plates. Softmax Pro GxP Software 6. SpectraMax microplate reader with UV absorbance detection (Molecular Devices) UV–transparent microplates Disposable: Corning cat. Fluorescence was measured with the Spectramax M5 plate reader (Molecular Devices). 0. Multimode microplate reader equipped with latest detections like fluorescence, absorbance etc. The reaction was monitored with a SpectraMax M5 plate reader photometer (Molecular Devices GmbH, Biberach a. S6. Page 1 of 8 . 1. 4 fmol/well, 384-well plate)−bottom Reading Speed (Kinetic) 96 wells: 11 s 384 wells: 22 s Luminescence Wavelength Range 300−700 nm Dynamic Range > 6 decades Sensitivity. Fe standards require only about 30 minutes but can be kept in 1x working solution for at least a week withoutAfter 15 min, the absorbance at 550 nm was recorded using a Molecular Devices SpectraMax M5 plate reader. Abs). Maximum signal with lowest background. 4: Test tube cover. Rating: 5. Please talk to your your local Tecan representative for the right configuration which suits your application demands. Description. 25 For direct lysis of clots by plasmin, plasmin (250nM) was added to the surface of clots and lysis monitored by theSteady-state affinity measurements were performed on an Analyst AD, Analyst HT, or SpectraMax M5 plate reader (Molecular Devices). Junichi Yamamoto a, Takumi Ito b, Yuki Yamaguchi a and Hiroshi Handa * c a School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan b Institute of Medical Science, Tokyo Medical. After 48-h growth at 25 C with agitation, the optical density was recorded using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). a SpectraMax M5 plate reader (Molecular Devices LLC) to detect the absorbance value at 450 nm. 8 cm x 41. 5. 2. was measured atdetermined450 nm using SpectraMax M5 plate reader. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). harvard. Reader Documentation Version No. Download a PDF version of the SpectraTest validation package. Uncontrolled Copy for Training and Reference Purposes Only . Fluorescence Microplate Reader Comparison Chart. Immunohistochemistry Four μm kidney sections were heated at 65 °C for 1 hr and deparaffinized in xylene, followed by rehydration in decreasing concentrations of ethanol (two washes in 100% ethanol, two washes in 95%, one wash in 70%, one wash in 50%,. After 2 h of incubation, the plates were shaken, and the absorbance was measured using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm (OD450). They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 0 . </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). Published Dec 16, 2020 | Updated Dec 16, 2020. To determine whether each drug had proved. We would like to show you a description here but the site won’t allow us. A sensitivity level of 0. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. The assay was performed on HDL collected. plates can be used in the SPECTRAmax PLUS 384. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. 2. The solids content was measured using a MA35 Moisture Analyzer (Sartorius AG). Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. Mutations in the α2–α2′ and α4–α4′ dimer interfaces affect the secondary structure of the DENV2C protein. Thermo Scientific™ Nunc MicroWell 96-Well Optical-Bottom Plates with Polymer Base have a solid polystyrene black or white upper structures with a polystyrene film at the bottom of the plate. Most papers they cultured bacteria to OD600>0. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. About. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. com 10. Dimensions 20" D x 16. Microplate Reader Shelf. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. 5. 1. The Back Panel Figure 1. The empty LNP matrix solution was made by mixing 300 μL of empty LNP solution with 45 mL diluent. 5 mL, pH 7. Assay was run using JAK1 JH1/JH2 and Omnia Y peptide 12 kit. 0 adjusted at rt containing 10% v/v DMSO) (100 μL) and a 2- or 5-fold dilution was. Thus, Eu 3+ has a detection limit of approximately 10–100 amol nowadays depending upon. Fuchs1,2,3* 1 Department of Biology, Tufts University, Medford, MA 02155, USA 2 Institute for Protein Innovation, Boston, MA 02115, USA 3 Program in Cellular and Molecular Medicine,. 5. Users can choose to process all plates within the magazine or select a specific number. 3. The Infinite and Spark multimode microplate readers are modular and upgradeable. Lucifer yellow concentrations in the donor and acceptor wells were measured in the last of this incubation. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. and 72 h using the CCK-8 protocol with absorbance readings at 450 nm measured with a SpectraMax M5 plate-reader (Molecular Devices. 2M posts 124. Application Area: Multi mode plate reader. com 9. Fluorescence was recorded in a Spectramax M5 plate reader at a 0. 316 grating motor driver faulty. Fluorescein 0. Find regional support. Unless reading plates from the bottom, put plates on the purple adapter to put them closer to the reading head. ) and had a resistivity of 18. . 0200-0729. Temperature variation ± 0. To avoid these issues and provide the best possible dynamic range between the highest and the lowest measurement values of your assay, fluorescence gain is typically adjusted on the sample with the expected highest signal output (e. Lysin A constructs were expressed in M. 7°C (gradient heating design). 99 was required for a valid standard curve. The Formazan crystals formed in each well were dissolved by adding 100 µl DMSO. 7°C and the bottom to 36. 3) At the bottom of the strip, click on "Show. Add to Cart. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. For a higher . the contents were carefully mixed by pipetting up and down. 10569C 2 C 12 cells were treated exactly as described for free [Mg 2+] determination, except that MgG was replaced by 5 μM safranine O. We carry both single-mode and multi-mode readers from manufacturers such as BioTek Instruments, Perkin Elmer, and SRU Biosystems. Highly selective for dsDNA over ssDNA or RNA. All kinetic reads were taken at 25 °C in nonbinding all black clear-bottomed Greiner 96-well plates covered with optically clear films and stirred for 10 s prior to each reading. plates in 150 ll OS minimal medium (Schnider-Keel et al. T o enable accurate quantita-tion, initially standard curves were plotted f or both Pi and. Results were expressed as absorbance units. The WT DENV2C and its single-point mutants L50S, L54S, L81N and I88N were. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. PPi. Thermo Scientific Microplate Reader Verification Plates are dedicated performance verification systems helping to verify and document the key performance parameters of Thermo Scientific Multiskan FC, Multiskan Sky, Luminoskan, Fluoroskan FL, and Varioskan LUX. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. 2000) supplemented with each chemical com-pound with the final concentration of 10 mmol l-1. Developed with Promega reagents to provide a. You can use it for many applications (OD, fluorescence, luminescence). SpectraMax Injector Cartridge with SmartInject™ Technology. To quantify the cell apoptotic degree, the harvested cells were stained with. Data reduction and calculation were performed with MSD Discovery workbench software (v 3. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 3. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. 5: Schematic of the back panel of a reader. Cell growth was monitored by measuring OD values at 600 nm using a SpectraMax M5 plate reader (Molecular Devices, Silicon Valley, CA, United States). Turn the reader on and secure the USB cable. The fluorometric performance of theAbsorbance is read with a SpectraMax M5 plate reader. 9. The plate can be shaken (icon right of thermometer, and see automix option in setup). The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications. There is currently no charge for using this plate reader. begin reading microplates with seven mouse clicks. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Chirag considers the SpectraMax M5 a multi-purpose reader that. 3. Detection modalities. Please contact Tatiana to reserve the plate reader time: [email protected] signals were scanned using a Molecular Devices SpectraMax M5 plate reader (M5, Molecular Devices, U. 0. Also refer to this Multimode Reader Comparison Chart. 2016 Aug 2; 7(31): 50027–50042. Patented Hybrid Technology delivers the sensitivity of filters with the flexibility of monochromators. 9000-0756. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Fluorescence values were used to calculate the percent inhibition of enzyme activity relative to maximal activity, PTP4A3 in the absence of inhibitor, and maximal inhibition, PTP4A3 in the presence of 2 mM Na 3 VO 4. com GeneBLAzer® Assay Setup Guide on theThe SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. edu to schedule training. Imaging opens up a range of applications for cell-based assays that cannot be performed on a standard plate reader. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Turn the reader on and secure the USB cable. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. After that, DCFH-DA was used to mark cells and the ROS level was determined by Spectramax M5 plate reader and five parallel samples were prepared for each group (Wu et al. Add 100 μL of sample media and standards (0, 1, 2, 4, 8, 16, 32, 64, 128 μM NaNO 2) to plate in duplicate. Blue, kinase (no inhibitor); green, no kinase. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. To detect the luminescent signals, 100 μl of each dilution was added into a 96-well plate before adding 50 μl of LumiGLO Peroxidase Chemiluminescent substrate (KPL, 54-71-00) to measure luminescent intensity by a SPECTRAmax M5 plate reader. Information on how to reserve the plate reader Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. To determine the DAR, STRO-002 in plasma samples were captured using Streptavidin Mag Sepharose Beads (GE Healthcare) coated with biotinylated. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Cultures were grown at 30°C and 900 rpm for 24 h, then OD600 was measured on a SpectraMax M5 plate reader to determine bacteriostatic potential. The luminescent signal was recorded on a SpectraMax M5 plate reader (Molecular Dy-namics). The average 526/470 ratio for the sample aliquots. edu The content of this site is published by the site owner(s) and is not a statement of advice, opinion, or information pertaining to The Ohio State University. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Description Figure 1. 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. Then, luminescence of samples was read in a Spectramax M5 plate reader with integration time of 5 s. Data were expressed as % control =. HeLa cells were seeded in a 6-well plate with standard DMEMfluorescence intensity in the supernatant was measured in a SpectraMax M5 plate - reader (Molecular Devices) at 485 nm excitation and 535 nm emission wavelengths. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Microplate Readers. Perkin Elmer Victor X4 Multimode Plate Microplate Reader 2030-0040 with PC. SpectraMax M5e Multi-Mode Microplate Reader. 5. 25 pM (0. GraphPad Prism was used to process the data and calculate the Km and Vmax values. 5 and 10 nm were used for excitation and emission, respectively. #655801. 314 low lamp level warning. Microplate automation and detection instruments for walk away operation and high quality results. 5: Schematic of the back panel of a reader. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. Thesupplyofhumidifiedair (zero air) was controlled by a solenoid valve with on and offLastly, Jurkat cells were least susceptible to Vorinostat treatment (all IC 50 > 50. After that, the medium was. Then the plate was incubated at 37 °C for 2 h. The degradation. Use of Attune Flow Cytometer, Leica Cryostat, M5 Plate Reader, and Typhoon 5 require training; please contact Cell_Bio_Operations at hms. Protein concentration of viral suspensions was measured using a Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific) with a mucin standard curve read on a SpectraMax M5 plate reader (Molecular Devices). 4: Test tube cover. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well microplates. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. We therefore gathered salient. Spend a little more and you can get an FTDI-based USB cable. The absorbance at a wavelength of 540 nm was quantified using a Spectramax M5 plate-reader (Molecular Devices) to determine the amount of gamma glutamyl hydroxamate, indicative of active enzyme. 0 and room temperature. The % PEG midpt and apparent solubility values (thermodynamic activity) were calculated as described previously. Luminescence values were measured on a SpectraMax M5 plate reader (Molecular Devices). Third this recommendation. High tolerance for contaminants. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 13. Wide range of applications The VersaMax reader covers a wide range of applications:To monitor the increase rate, fluorescence measurement is then performed at 37°C at 10-s intervals for 10 min with Ex/Em 450/510 nm in the plate reader (Spectramax M5 plate reader, Molecular Devices, For each bacterial sample, we performed the reactions in duplicate and averaged the results. The dual monochromator optics allow. Menu. no. [4] The IC 50 value of each drug was calculated by the Logit approach. CD spectra were recorded using a Jasco-715 circular dichroism spectrophotometer at 20 °C. 6. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through. Riss, Germany) at 330 nm and room temperature for 30 min. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 5: Schematic of the back panel of a reader. After MMP9-antibody complex formation, substrate (20 μM for human and mouse assays, 10 uM for rat) was added and fluorescence was monitored in kinetic mode at 37°C on either a SpectraMax M2 or M5. On microplate readers,. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. $4,995. 4. SpectraMax M-series Microplate readers. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. The plate was read by a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm with 620 nm as reference. RNA for the RNA-Seq experiment was obtained exactly as described previously for the quantitative RT-PCR. 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 10569The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Open and turn on the laptop. Incubation.