The advanced optical and. In addition, the SpectraMax iD5The SpectraMax® L Luminescence Microplate Reader is a high-performance microplate reader that measures luminescence in 96 and 384-well microplates. Instrument settings used with the SpectraMax M5 Microplate Reader. Most papers they cultured bacteria to OD600>0. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. Blank subtraction and standard curve fitting were done automatically by the software. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The 2-channel configuration is available with 0, 2, or 4 injectors. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. 0 out of 5 $ 5,999. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). Figure 3. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Absorbance (optical density, OD) was read at 570 nm and 605. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. Omnia® Compatible Microplate Reader Documentation Version No. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 000 Euro/Dollars for a high-end multi-mode microplate reader with several detection modes and the best technology for highest sensitivity and fastest detection. Maximum signal with lowest background. 0 out of 5 $ 5,999. The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Microplate Readers BioTek Epoch 2 Microplate Reader. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. For more sample throughput, both readersSupplier: Molecular Devices. The dual monochromator optics allow. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. edu) to be added as a trained user in iLab. Dual-Glo luciferase assay system (Promega Cat. Products : General, Microplate Readers. Cell Analysis. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. 100 readings per well gives optimal Z’ values for this assay. The University of Rouen in Normandy uses the SpectraMax iD3 and. Add to Cart. An enhancer is provided in the kit where applicable to further reduce background fluorescence and to increase the. 72 MB. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. microplates or in a cuvette at a. Page 28 Chapter 5: Read Modes and Read Types 1. The plate. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. 0 out of 5 $ 23,995 $ 22,495. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 304 signal level saturation. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. Introduction. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Three low volume 24-well bottom slides. Sale. The Quant-iT PicoGreen dsDNA Assay Kit, when run on a SpectraMax microplate reader with SoftMax Pro Software, is a quick, sensitive detection method for double-stranded DNA. M2. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. ¨0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingThe SpectraMax iD5 Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. In this product video, see how the SpectraMax M5 quickly reads samples. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Microplate reader assays requiring infrared (IR) or red-shifted. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Chapter5:ReadModesandReadTypes 0112-0115J 27 Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 5 87. flexibility of monochromator-based systems. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. Ease of use. SpectraMax Mini User Guide. Time-. Examples: “Press carefully to engage,” “ Do not press down. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. The SpectraMax® ABS Plus Microplate Reader can run . The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Supplier: Molecular Devices. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Fill a clean plate with water. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). 7°C and the bottom to 36. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Spectranet customer portalthe SpectraMax® M Series Multi-Mode Microplate Readers, for . The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. Italic and boldface type are used for emphasis. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. microplate reader. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. We would like to show you a description here but the site won’t allow us. both cuvette-based and microplate reader applications Microplate Readers. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96-. Highly customizable low volume. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalSpectraMax Microplate Readers. HTRF-compatible SpectraMax Multi-Mode Readers. 997 (n = 3). Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Fluorescence analysis of the colonies from the dilution series was performed in a microplate reader (SpectraMax iD3). SAN JOSE, CA — Molecular Devices, LLC. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. 2. Download PDF. Microplate Spectrophotometers. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published0200-6262. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Find regional support. The dual monochromator optics allow the widest range of. configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking andThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expandthe boundaries of your research capabilities. Use the provided 3. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Introduction. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader. SpectraMax Microplate Readers. Temperature control and shaking come as standard. Lysozyme contains two tryptophan residues that. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. their consistent performance, reliability and durability. Download the SpectraMax Mini Reader Plug-in and associated guides. Figure 6: Transcreener ADP2 FI assay principle. Open the top cover of the instrument. Type: Clear: Add to Cart. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 7°C and the bottom to 36. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. For readers without a cuvette port, simply check the box next to ‘PathCheck’. With optimized reagents, option to operate the. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Add to Cart. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. For biological applications involving small samples,Minimum volume and maximum throughput with 96- or 384-well microplate compatibility. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. A preconfigured protocol is available in the software to. Detailed specifications of each model reader are reported in Section. In addition, the SpectraMax® iD5 can be expanded to include bottom. Fluorescence Microplate Reader Comparison Chart. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. We would like to show you a description here but the site won’t allow us. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. The SpectraMax® ABS Plus Microplate Reader can run . AlphaScreen. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. com 4. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. 1 software Download page. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. with SpectraMax Microplate Readers Introduction Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Due to elevated security of a Windows 7 64-bit operating system, the USB instrument drivers may not be installed automatically. 58% for the detection module and 2. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. This is equivalent to having 511 built-in filters, The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Sensitive fluorescent quantitation of DNA with the Quant-iT. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. All . SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. This unlocks the transport slide. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Other technologies such as imaging. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. 1. ATP standard curves read on SpectraMax microplate readers. flat meniscus), but is approx 15 mL. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. 2 software: see SoftMax® Pro 7. See SpectraMax® Microplate Reader User Manual Download Page. SpectraMax Spectrofluorometers. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. 0 out of 5 $ 2,999-20%. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Bookings are free, but you will need to use iLab to book. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. 58% for the detection module and 2. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax i3x. We use Spectramax M5 microplate reader in our lab. 2 only) SpectraMax M-series Microplate readers. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Additionally, user-installable options allow the SpectraMax i3 System to. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Step 1: Turn on your microplate reader. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. SpectraMax L Microplate ReaderThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. (A) AccuBlue Pico kit; (B) AccuClear Nano kit; (C. Click SoftMax Pro Software User Guide. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Path Check Pathlength Measurement every Gemini EM Reader. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. 20 μL of Component A was added to each well of the microplate. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Step 2: Launch SoftMax Pro Software. IMAP FP Settings in SoftMax ProSpectraMax M5 Multi-Mode Microplate Reader. Download PDF. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMinimum volume and maximum throughput with 96- or 384-well microplate compatibility. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. 33 Dramatic aggregation of Alzheimer A b by Cu(II) is induced by conditions representing physiological acidosis . Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Summary of Contents for Molecular Devices SpectraMax M2. 0200-6263. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. 100 fmol 1536-well (8 µL) 0. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. #6590-0131) Fluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 2. Figure 2-23: Bottle holder To install the bottle holder: 1. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. Each sample has a discrete sample beam and reference beam so that each well is measured directly. SpectraMax 250 User. 003 fmol 0. Win7 Manual Spectramax-L or StakMax Driver Installation after Initial Install. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. Molecular Devices SpectraMax M2 Multimode Plate Reader. The following document is intended to demonstrate setup of this instrument. Highly customizable low volume. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Temperature control. We would like to show you a description here but the site won’t allow us. We would like to show you a description here but the site won’t allow us. \ Figure 3. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. The plate reader can measure multiple readouts, including UV and visible absorbance. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Nuclear Receptor. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Table 2 summarizes the results for the uniformity test. 300 µL of Detergent Compatible Bradford reagent was added to each well. . Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. 0 out of 5 $ 11,999. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. The calculated LOQ values were 0. . FI assay. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. 0 out of 5 $ 5,999. . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate 312 gain calibration failed. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. In addition, SoftMax Pro Softwareluminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. 314 low lamp level warning. Offer Ends In: 18 Days 19: 38: 21. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Contact our support team with your questions regarding Molecular Devices products and applications. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Published Dec 16, 2020 | Updated May 19, 2023. SpectraMax Microplate Readers. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. When the user applies a set temperature of 37°C, the top is heated to 37. The dual monochromator optics allow the. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Positions H21-K22 contained plate blanks of PBS only. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Microplate readers’ standard detection modes. Other technologies such as imaging. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. This pathlength correction is accomplished only with the use of. In addition, the SpectraMax iD5 can be expanded to. Compare plate readers The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The SpectraMax® i3 is Molecular Devices latest multi-mode microplate reader which delivers several applications including; Ultra-Violet to visible absorbance detection, fluorescence intensity and multi-colour luminescence. The plate reader can measure multiple readouts, including UV. Figure 1. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The drawing shows a standard microplate of 15 mm height covered with a lid. Examples: “Press carefully to engage,” “ Do not. Brochure. Example: [Drawer]. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an.