For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. The opaque walls prevent stray light from being detected from neighbouring. It has a 340-750nm Spectral Range. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Optional 12-way manifold) 5032336. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 5 ng per well. 5L) MultiWash+. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 96-well plates - Filter plates with 0. The Versamax Microplate Reader is built around a grating monochromator. 96 well plate injection time with SpectraMax® L plate reader. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. It also integrates with the. robotics-automation-compatible. Mode Plate Reader. Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. In addition to increased throughput, microplate formats enable researchers to miniaturize their analysis (using less sample and reagent) to study regulatory events and. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Controlled by the computer. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human AT1R receptor. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. x. Features. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. The Phosentix MicroQC™ Plate is an advanced microplate reader analysis tool that offers exceptional multi-modal functionality. x. 0025 OD from 0. Select models. 5 fg per well to 0. Working in. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. We would like to show you a description here but the site won’t allow us. Selects either 96-well or 384-well plate for a stand-alone read. After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. 5 ng per well. 96-well microplate compatible Well read area range: D4. Figure 3. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. Click SoftMax Pro Software User Guide. Place the microplate in the drawer. Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. The plate reader optics are tunable for. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. x. Detection of KASP genotyping reactions. This reader's dual monochromators for. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. • 0. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The VersaMax reads 96-well microplates. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. Figure 2. A control well was set up to verify. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. Well scanning With well scanning, multiple points within each well can be read, providing a high. An industry-renowned instrument for monitoring GPCRs and ion channels. 96- and 384-well. 190. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. T = I/I 0 and %T = 100 (T). Self-righting base, up to 40 degrees past center without dispenser. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Access this by going to. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. Packed 25 plates per sleeve, 2 sleeves per case. 005 OD Normal and Rapid modes, 96- and 384-well plates;. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. #iD5-STD). 1 – 2. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Intuitive graphical user interface makes each assay extremely easy to set. a white 96-well half-area microplate (Corning cat. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. Description: Enter plate name (optional)(2) Wild‑type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β‑catenin pathway Molecular medicine reports January 1, 2023 Hu X, Zhang R, Yao J, Mu B, Zhao C. No tools required to assemble. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. 0 Optical system Filters Power requirementAfter reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. Capabilities. View Product. A = Log 10 (I 0 /I). 2. We would like to show you a description here but the site won’t allow us. M2. % transmittance of plates used in a plate reader. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Range of 96, 384-well SBS microplates . • To avoid removing loosely attached cells don’t wash if the background signal is not too highTeams measured absorbance at 600 nm (OD600) and GFP in a plate reader from four technical replicates per biological replicate (for a total of eight replicates and fitting on a 96-well plate) at. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. 96-, 384-, and 1536-well microplates. Molecular Devices' microplate readers. 96- or 384-well microplate compatibility. 00 USD. #ABS PLUS) Methods. 2. Optimized for fluorescence microplate readers. It also integrates with the powerful SoftMax Pro Software for. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Artel MVS Bundle DM Std/800TSNB Reader Details. as well as all other Molecular Devices' microplate readers. Populate the following info under Description. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. For a 96-well plate it takes a total of 27 seconds to switch between wells and then you have to add the actual injection time based on the injection rate entered by the user. of 3 wells of a 96-well plate, from 1 of 3 independent experiments. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. What are the dimensions of the plate? Code. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. 384-WELL AND 96-WELL 10MM-HEIGHT STERILE MICROPLATES. Instrument settings can beCorning 96-well black microplates with clear bottom (Corning cat. The equation that allows one to calculate absorbance from % transmittance is. Validation made easy -1) Select the correct plate and the wells that you want to measure. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. 0 . measurements in compatible Molecular Devices® microplate readers. A. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Description. Glass microfiber: Use for cell harvesting and binding assays, liquid scintillation counting or high loading, DNA binding and purification; Melt blown polypropylene: Use for pre-filtration; Cellulose acetate: Use for low protein binding,. 1. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. #3603) SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. • Easy and gentle immobilization of biotin. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. #E010) Pyroplate 96-well microplate (ACC cat. Plate. The 6-well plates were incubated at 37ºC for 9 days before picking. ₹ 1,80,000 Get Latest Price. 97 independent and fixed optical fibers can achieve a mean time between failures (MTBF) of 20,000 hours, which keeps the microwell plate still during the entire reading cycle,. 5. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Infinite possibilities. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 306 plate air cal. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 24-, 96-, 384-well plates and microwell strips; Manifold Channels: 96-well washing: 8-well (1x8) manifold, 2x8-well manifold, 12-well (1x12) manifold, 8-well short tube (1x8) manifold. This is equivalent to having 511 built-in filters, The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. Dynamic range > 6 decades . PURPOSE. Dual injectors for kinetic assays. This eliminates the need for standard curves, and 96–well: 3 mP 384–well: 3 mP 1536–well: 6 mP 1 LUMI Glow luminescence detection for different plates 0200-7014POS Glow for 96-well plates 96–well: 1 pM 96–well: 1. #C1500) Control standard endotoxin (ACC cat. Published Dec 16, 2020 | Updated Dec 16, 2020. When the user applies a set temperature of 37°C, the top is heated to 37. Return to top. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. µl) was added to each well and incubated at 37°C for 1 h. Plus 384. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Microplates, cuvettes (via adapter) Detectors. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. Turn on Plate Reader and wait for it to complete its start-up routine. 00. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. Temperature control: 4°C above ambient to 45°C. adjust the reader. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. Elevated well walls for easier sealing Round bottom formats Square and round well formats Conform to SBS standards Lids and cap mats available Packed in sleeves of 6, 24. The top- and bottom-reading Gemini EM Microplate. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. We would like to show you a description here but the site won’t allow us. 100 fmol 384-well (75 µL) 0. Make sure toPlate reader instructions Computer. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. 993 (Figure 3), validating the assay’s usefulness over a wide range of viable cell numbers. 1. Cayman Chemical. 1% in white 96- and < 0. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The Absorbance 96 Plate Reader is designed to run everyday assays with ease and speed. 3) At the bottom of the strip, click on "Show. 00 USD. x. Reaction formats The MagMAX™-96 DNA Multi-Sample Kit can accommodate a broad range of throughputs. An industry-renowned instrument for monitoring GPCRs and ion channels. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. #R8195. Plate types Flat and round 96-well plates Data output Export to PC Detection system Single channel silicon photodiode Reading speed 25 seconds Software SoftMax Pro Software Shaking None Temperature control None Linearity 0. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. Other technologies such as imaging. 5 OD at 492 nm Computer interface USB 2. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume differences that may. This study investigated the efficacy of fermented seaweed (Eucheuma cottonii) on the remediation of fluoride-contaminated soil. Cell viability and cytoxicity assays. Microplate Readers. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. SpectraMax ID5 Plate Reader (Molecular Devices). 5A spare fuses, 2 cleaning needles, tubing sets for all bottles, cleaning tool for needles. . VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Compare this item. 0001 ABS (calculated) • Light source: Halogen lamp, 8V/50W • Wavelength range: 405, 450, 492, 630 nm, 4 more filters optional. Important things to consider when purchasing a multimode microplate reader. President of Molecular Devices. 2b,c. . Making it possible to perform kinetic analysis of solid. Wells of 96-well plates (NEST) were pre-coated with 100 μg/ml of collagen solution for 1 hour. Fluorescence Microplate Reader Comparison Chart. Important things to consider when purchasing a multimode microplate reader. Compare Tool. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. Published Dec 16, 2020 | Updated Jun 22, 2023. 96- & 384-well automated washer, multiwash™+ automated washer, 96- and 384-well plates, washer maintenance kit. Conventional technique: subjective, time-consuming. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc. 6-384 (96- and 384-well with pipetting) 96-well microplates. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. 01. This approach enables uniform light dose application to 9 wells (a 3-by-3 matrix in a 96-well plate) simultaneously, which can be leveraged to shorten the duration of PDT experiments (Fig. Instrument settings can. Plate SpectraMaxiD3,iD5,i3x,i3,M2,M2e,M3,M4,M5,M5e,Plus. S. 1. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. 500 OD ± 3. 300 µL of Detergent Compatible Bradford reagent was added to each well. Align the two slots on the bottle holder with the two pegs on the rail. 000 to 2. The picked. Erik Koppes, Richard Haas, Jerry Vockley, Lina Ghaloul-Gonzalez approximately 5,000 cells were seeded in 4 replicates in 96-well plates. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. tools to expand the boundaries of their research. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. 5 µL of each sample was pipetted into a 96-well plate for PCR. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. S. 2% in. REF (SpectraMax. These are the default kind of cuvette & 96 well plate stocked by the lidstrom lab as of 11/2013. Up to 96 well plates, there is often a good signal that can easily be detected, and 96 wells are measured comparatively quickly. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. On the day of the assay, the plate was equilibrated. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. The 8-channel read head allows for fast read times which means a 96-well plate can be read in as little as five seconds. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. A = 2 - log 10 (%T). It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). This page contains the. The advanced design of the Sunrise plate reader includes. Top and bottom reads are. This robust system is capable of reading 96 wells, each with its own sample beam and reference detector. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Some will only support one well density (e. 3% in white 96- and 384-well microplates < 3 x 10-5 (white plates) < 3 x. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 000 OD ± 1. 96-, 384-, and 1536-well microplates. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution forThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. x. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. Corning. Polypropylene deep well plates, round bottom. HeLa. Related Products: 96 Well Plate Reader. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. May require LS-50B…. Including injection time, it takes approximately 68 secs. White light and fluorescence images of the 6-well plates were captured with the ClonePix 2 System. High sensitivity and speed are required for these plate formats if you wish to examine quick kinetic measurements such as calcium fluxes or applications with small signal. x. 312 gain calibration failed. Products : General, FilterMax F5 reader. We would like to show you a description here but the site won’t allow us. Unparalleled performance on a personalized platform. 020 fmol 0. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. Includes PC and software. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. • Photometric range: 0. Shaking: Linear, orbital, and double orbital. Single or dual wavelength read, kinetic readings, automix, endpoint, and blank pattern, curve fits included with PC Software. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. x. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. 96-, 384-, and 1536-well microplates. Microplate Reader Ordering information Item Description Part number FLIPR Membrane. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). Suggested plates are black walled. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . With more than 40 years of plate reader expertise and life science research, we’ve amassed an extensive. Lucid Scientific. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. DynaPro ™ Plate Reader III. Read up to 2 wavelengths in endpoint absorbance for 96-well plates. Published Dec 16,. Product Compare 0. Endpoint, kinetic, spectrum. flat-bottom: 50-60 µl. Two different agonists, acetylcholine and carbachol, were tested. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. Description. SoftMax Pro GxP Software version 5. minutes and begin reading microplates with seven mouse clicks. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. Clear plates will give more cross talk between wells. Supersedes 1. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. Order Now.