The SpectraMax M2 and M2e are versatile and reliable. Molecular Devices. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. 00. A: Microplate Preparation. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. Key applications: ELISA, nucleic acid, endotoxin testing. E Models Only) 69. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. ATG provides repair, maintenance, and contract services for laboratory research and bioprocess instruments. Do not touch the clear surfaces of the cuvette. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 04 to 3. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. flexibility of monochromator-based systems. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. Cell viability was quantified by measuring the absorbance of the solution at 570 nm using a SpectraMax® M5 microplate reader (Molecular Devices). Temperature variation ± 0. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplateinstalled if you are using a SpectraMax ABS microplate reader **SoftMax Pro Software version 7. Depending on which. SpectraMax M3 M4 M5 M5e SpectraTest FL1 revA. Maximum signal with lowest background. 00. Table of Contents. Lower limits of detection for ATP determined using the ATPlite . The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. Fifteen different automated tests are provided. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. ) by nonlinear regression using. , USA). It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. 6 0. 0 out of 5 $ 24,695. SpectraMax® M5e. 304 signal level saturation. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . 303 unable to cal dark current. Microplate Readers include the M3, M4, M5, and M5e models. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. Three independent experiments were performed. SpectraMax® ABS Plus. 2 only) SpectraMax M-series Microplate readers. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 2. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. 0 μL) instead of the sample solution was applied as control group, and. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Here the delta mP was 336, and Z’ factor was 0. It measures the optical density (OD) of samples in a microplate or in a cuvette at aThe cells were then washed three times with PBS, and the fluorescent intensity of the cells was measured with a SpectraMax M5 microplate reader (Molecular Devices, USA) at an excitation wavelength of 488 nm and emission wavelength of 525 nm. Introduction. . Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Molecular Devices | Life Science Applications | Bioanalytical. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. When a SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. Western blotting assays were conducted according to previous studies . Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Standard SDS-PAGE was performed with a 10% (w/v) separating gel and a 4% stacking gel. Compact and tunable absorbance microplate readers DATA SHEET. Introduction. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. PURPOSE. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. 7. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The following document is. 4. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Thank you!. Microbiota DNA was. Cables. book Page 7 Wednesday, July 7, 2010 2:09 PM. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. . Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. SpectraMax M Series multi-mode microplates are configurable readers with triple. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Setup for GeneBLAzer® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. 6 for further experiment, so I'm confused which i would choose for OD value determination. Absorbance was read on a Spectramax M5 Microplate reader at 450 nm. Access this by going to Help >. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. 7 cells (6 × 10 5 cells/well in 6-well plates) were pretreated with of 3,3′,4,5′-TMS or 3,4′,5-TMS at 10 and 50 µM for 4 h, followed by the addition of LPS (1 µg/mL) for 12 h. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. SpectraMax® ABS. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Five preferred detection modes. S. VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® Plus 384 Absorbance Microplate Reader; SpectraMax® L (software version 5. When a The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Category 2 and 3 microplate readers use gradient heating as well. Read one sample or up to 384 inThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Qualify the fluorescence performance of the SpectraMax . The SpectraMax L Microplate Reader was set up for endpoint reading with an inte-gration time of 1 second or 0. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 63 1. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. : 2 Oct 12 Page 2 of 10 Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Incubation. Molecular Devices Spectramax M5 Microplate Reader. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). AlphaScreen. Spectramax Multi-Mode Microplate Reader Performance Specifications. The SpectraMax® ABS Plus Microplate Reader can run . When a feature or capability applies to only certain readers, this exception is noted. The guide also covers the software features, the specifications, and the accessories of the systems. Amino acids assay. 72 MB. These robust readers have been placed in labs from Antarctica to. reader in the visible range between 405–750 nm with NIST and NMI. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. 2. Figure 3. The assay window spanned four orders of magnitude. Temperature control range from Ambient +4º to 50ºC. The acceptance. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 1. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. SpectraMax® Aquamax. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. spectramax m5 multi. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. SpectraMax® M5 Multi-Mode Microplate Reader (cat. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-,. 2. 5 Compatible microplate readers FP Plate Reader SpectraMax® M5/M5e Multi-Mode Microplate ReaderSpectraMax M5 Multi-Mode Microplate Reader; Method. edu) to be added as a trained user in iLab. • Tel. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. . The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. FlexStation® 3 Multi-Mode Microplate Reader. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. Plasma Tau was determined by high-performance liquid chromatography (Shimadzu ®, model LC 10AD) using a Shimadzu model RF 535 fluorescence detector. All associated StakMax® stackers. Fluorescence from the Alamar blue® assay was detected by SpectraMax M5 microplate reader (Molecular Device Inc. Levels of immunoreactivity were normalized to. The calculated LOQ values were 0. 1. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. The drawing shows a standard microplate of 15 mm height covered with a lid. SpectraMax Glo Steady-Luc Reporter Assay Kit. 87. SpectraMax M5 microplate reader (Molecular Devices, California, USA) with excitation at 450 nm and emission at 570 nm. Bookings are free, but you will need to use iLab to book. Figure 4: Transcreener ADP. e. Learn how you can utilize microplate readers to drive landmark discoveries. 1. 1 & 7. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax® iD3 Multi Mode Microplate . Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. We would like to show you a description here but the site won’t allow us. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Choose from a. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 7 cells and HEK293T cells were seeded at the density of 2 × 105 cells per well in a 24-well plate overnight. ® reader to the computer. Bookings are free, but you will need to use iLab to book. Molecular Devices SpectraMax M5 ; Molecular Devices SpectraMax M2. Here the delta mP was 336, and Z’ factor was 0. Download PDF. 1 is GxP edition only; Protocols for the ABS1 Validation Plate. Wide range of applications. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. 05 3. Learn how to set up protocols, customize reports, export results, and more with this PDF document. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. Ltd. When a feature or capability applies to only certain readers, this exception is noted. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Modethe introduction of the SPECTRAmax PLUS microplate spectrophotometer and its PathCheck feature, the ability to determine the pathlength in each well of a microplate and automatically normalize the absorbance value to a 1 cm pathlength. Wide range of applications The VersaMax reader covers. We would like to show you a description here but the site won’t allow us. 1. 96-1536. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Turn on the microplate reader. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. This. free tracer decreases, resulting in an. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. AquaMax® 4000. Fluorescence Microplate Readers. The CellTiter-Glo assay from Promega uses luciferase. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Biotek FLx800 Fluorescence Microplate Reader; Related products-20%. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices. both cuvette-based and microplate reader applications on the same instrument. 6: bioNova científica, s. When the user applies a set temperature of 37°C, the top is heated to 37. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. When a feature or capability applies to only certain readers, this exception is noted. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Please refer to the Obsolete Microplate Reader Models for more information. The CDWIA was performed on each. 312 gain calibration failed. Microplate Readers are modular and . Choose from a three- (M3), four-. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 9000-0756. The cells were then treated to induce apoptosis. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. "With this complete portfolio of multi-mode configurations, we. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. Use similar settings for other Molecular Devices readers with fluorescence detection mode. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. The assay applies fluorescence polarization to quantify Fc-containing IgG. 5. The day before assay, assay buffer was prepared as described in the product technical manual. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 2. Shimadzu 2450 UV–Vis spectrophotometer and SpectraMax M5 microplate reader were used to perform the optical characterization and measurements. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. free tracer decreases, resulting in. 6: bioNova científica, s. (The i3x and Paradigm readers require the. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. The CellTiter-Glo assay from Promega uses luciferase. Lift up the lid over the chamber to insert or remove a cuvette. Italic and boldface type are used for emphasis. For the Live/Dead assay, they were treated. Molecular Devices Spectramax M5 Microplate Reader. Description. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. Western blotting assay. FlexStation™ I. Uncontrolled Copy for Training and Reference Purposes Only . Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. com. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. B: Selection of Excitation Wavelength. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. The samples were boiled for 5 min with 1 ×. The detection of lactate dehydrogenase releaseBriefly, algae were harvested, centrifuged, and re-suspended with an equal volume of methanol at the end of the experiment. Molecular Devices Spectramax M5 Microplate Reader. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. 67. View the Molecular Devices website. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Five preferred modes for diverse bioresearch applications. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Expanded wavelength coverage. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. For more sample throughput, the system can Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. 0 out of 5 $ 2,499 $ 1,999. Five preferred detection modes. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. If you do not have an iLab account you must first make one: The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Fill a clean plate with water. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. Published Dec 16, 2020 | Updated Dec 16, 2020. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. CAT activity was measured using SpectraMax M5/M5 Microplate Reader (Molecular Devices, MDS Analytical Technologies). For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. When a FlexStation® 3 Multi-Mode Microplate Reader. FlexStation™ I. We use Spectramax M5 microplate reader in our lab. Wide range of applications. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader Multimode Readers Comparison Chart. Microplate Readers Fisher Scientific AccuSkan FC Microplate Reader – 2020. 0 OD for earlier models. Table of Contents. Optical absorbance. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. 7°C and the bottom to 36. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Retrieving Each. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 7 cells were co-transfected with 0. Sale price $16,495. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Molecular Devices. 0 and 0. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Bookings are free, but you will need to use iLab to book. iD5 i3x M5 Paradigm Delta mP 69 85 92 88 Average StDev (mP) 1. Detailed specifications of each model reader are reported in Section 7, “Specifications”. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Read the Cuvette 1. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. 2. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance,. To block unwanted residual excitation light and minimize background interference, a 570 nm. Add to Cart. Supplier: Molecular Devices. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. 1. The SpectraMax M2 and M2e are versatile and reliable. Microplate Readers Include the M3, M4, M5, and M5. (Shanghai, China). The K d was calculated with GraphPad Prism software (GraphPad Software Inc. Documentation Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. 2. Fluorescence Microplate Reader Comparison Chart. D. The spectrophotometric. Molecular Devices. Next, add 10 μl of the MTT labeling reagent (5 mg/mL) to each well for 4 h. The dual monochromator optics allow the widest range of applications to be utilized for bior. 2. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. 0 . 40266 Email: drugdiscoverytech@lifetech. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. 315 can't find zero order. Fluorescence intensity was detected by. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. 1 second. 40266 Email: drugdiscoverytech@lifetech. All assays were performed in at least three individual experiments, each comprising no. Flexible for 6-well to 384-well microplates and cuvettes. Table of Contents. STAKMAX. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. 1. 1. Example: [Drawer]. Sale price $18,499. Molecular Devices Spectramax M5 Microplate Reader; Related products. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. Also refer to this Multimode Reader Comparison Chart . The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 1** Version 7. The absorbance was measured at 570 nm using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). SpectraMax® M5e. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Turn on the microplate reader. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. 0. l. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. Example: [Drawer]. Microplate Readers include the M3, M4,. com A. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. 1 Version 7. Devices’ StakMax® Microplate Stacker in a matter of minutes and .