The 8-channel read head allows for fast read times which means a 96-well plate can be read in as little as five seconds. x. SoftMax Pro GxP Software version 5. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. 01. Other. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The opaque walls prevent stray light from being detected from neighbouring. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. #SpectraMax L Config) Methods. Fluorescence Microplate Reader Comparison Chart. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Other technologies such as imaging. 1 x plates; Instrument dimensions. Automated live cell imaging – cell counting and confluence. In SoftMax Pro, Dual Read mode was selected. This approach enables uniform light dose application to 9 wells (a 3-by-3 matrix in a 96-well plate) simultaneously, which can be leveraged to shorten the duration of PDT experiments (Fig. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY . 2. 0. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. Wells to read. This is equivalent to having 511 built-in filters,The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 0 OD. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Cell viability and cytoxicity assays. External Dimensions without lid (mm). Microplate Readers. 5A spare fuses, 2 cleaning needles, tubing sets for all bottles, cleaning tool for needles. Glass microfiber: Use for cell harvesting and binding assays, liquid scintillation counting or high loading, DNA binding and purification; Melt blown polypropylene: Use for pre-filtration; Cellulose acetate: Use for low protein binding,. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. Microplate Readers include the M3, M4, M5, and M5e models. Microplate Spectrophotometers. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume differences that may. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. the Molecular Devices SPECTRAmax® 190 and 250. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Click Create. High tolerance for contaminants. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. 0 . These include real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR,. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. 300 µL of Detergent Compatible Bradford reagent was added to each well. Spectrofluorometer from Molecular Devices® provides a flexible environment. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Artel MVS Bundle DM Std/800TSNB Reader Details. 005 OD Normal and Rapid modes, 96- and 384-well plates;. 00. the Bio-Tek PowerWave™ 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Multimode microplate reader equipped with latest detections like fluorescence,. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Quantitative, whole well cell confluence for every well. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. Well scanning With well scanning, multiple points within each well can be read, providing a high. Two high-energy xenon flash lamps: Reading capabilities. 5°C at 37°C. 9000-0756. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED. B. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. This robust system is capable of reading 96 wells, each with its own sample beam and reference detector. Site. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro Software. 2% in. to each well. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). It also integrates with the. Top and bottom reads are. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Published Dec 16, 2020 | Updated Nov 07, 2022. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. 46Click the Read button in the SoftMax Pro 8-channel (96) for FlexStation 3 Reader • 8-channel pipettor • (10) racks of 96-well, FlexStation Pipet Tips (Black) • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettor • (10) racks of 384-well, FLIPR® Tetra Pipet Tips (Clear) • 384-well yellow plate 0200-6183 Consumables ELISA Plate Readers / Microplate Readers. A. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. The. It offers extremely low absorbance background, excellent. This can increase throughput and efficiency, thus saving time and space in the lab. Making it possible to perform kinetic analysis of solid. 1 fmol luciferase per well. The onboard luminescence detection mode may be used, or, for higher sensitivity, the reader can be equipped with a NanoBRET detection cartridge that offers a 4-fold improvement in limit of quantitation. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. Never order or replace filters to optimize an assay again!6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. 340 PC384. This offers the potential for fast and convenient measurements of FAAH activity using a 96-well plate spectrophotometer. #CA961) Methods. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Absorbance cuvette port. Bioz Stars score: 86/100, based on 40 PubMed citations. VMax . However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. 45 µm PVDF. x. High-throughput, automated dynamic light scattering. and glow luminescence modes in 96- to 384-well plates. D. x. Fluorescence Microplate Reader Comparison Chart. 1. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. µl) was added to each well and incubated at 37°C for 1 h. Next-generation luminescence plate reader. Related products. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. Description. Luminescence applications and assays. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. of Dispensers:Molecular Devices deliver high throughput while showing good correlation with manual. 00. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc. Important things to consider when purchasing a multimode microplate reader. These are the default kind of cuvette & 96 well plate stocked by the lidstrom lab as of 11/2013. 2. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. 96-, 384-, and 1536-well microplates. one 96-well sample plate can be controlled from 15–40°C,. 020 fmol 0. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. Published Dec 16, 2020 | Updated Dec 16, 2020. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. 3 OD at 200 nm. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Range of 96, 384-well SBS microplates . White light and fluorescence images of the 6-well plates were captured with the ClonePix 2 System. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. Each wellThe Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. Inconsistent results: cannot determine whole well confluence – well after well. Temperature control: 4°C above ambient to 45°C. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. Microplate optimization and read height optimization were performed for best results. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. 3) At the bottom of the strip, click on "Show. The eight-channel system delivers high precision and speed when reading 96-well microplates for single endpoint measurements and kinetic assay data gathered over time. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. x. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues . These robust readers have been placed in labs from. 5 pM 1 0200-7015POS Glow for 96- and 384-well plates 96–well: 1. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Click Add to create the plate. Compare this item. A standard 96 well plate was set up as described in Protocol Table 4. MCF7 cells treated with. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Bay Gray powder coated metal back plate, column, and base. 0 . Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. These lab workhorses are used in drug discovery. All trademarks used herein are the property of. C. Capabilities. Learn more. Reader Documentation Version No. Figure 3. 96- and 384-well. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. 000 OD ± 1. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Selects either 96-well or 384-well plate for a stand-alone read. 0 . Figure 2-23: Bottle holder To install the bottle holder: 1. with Quartz ABS Plastic. Figure 1-2 Match chamfered corners of slide and adapter 2. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. 000 to 2. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Please refer to the Obsolete Microplate Reader Models for more information. 6. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Single or dual wavelength read, kinetic readings, automix, endpoint, and blank pattern, curve fits included with PC Software. Z. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. In the latter case, 8 wells in each column are perfused in series and the 12 columns are. M5. 001 ABS (displayed), 0. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. #C1500) Control standard endotoxin (ACC cat. It has a 340-750nm Spectral Range. 306 plate air cal. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. 96-well Molecular Devices P/N 42-000-0117 HE™ High Efficiency microplates, 384-well Molecular Devices P/N 0200-5201. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. validation, maintenance, and repair documentation. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. . A = 2 - log 10 (%T). Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). They can be read by most microplate readers. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. The same volume of genotyping mixture (2x KASP Master mix plus KASP Assay mix) was added to the each well. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. Description: Enter plate name (optional)(2) Wild‑type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β‑catenin pathway Molecular medicine reports January 1, 2023 Hu X, Zhang R, Yao J, Mu B, Zhao C. for molecular biology. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. x. 96-well plates - Filter plates with 0. For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. REF (SpectraMax. wells across each plate • Enables viewing and tracking of every well in every plate • Reveals additional information on cellular. Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Step 3: Click “Settings” and a screen will appear (Figure 1). Make sure toPlate reader instructions Computer. Multimodal plate reader: Yes: 25 mm: Yes—Max. Wells of 96-well plates (NEST) were pre-coated with 100 μg/ml of collagen solution for 1 hour. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and software solution forThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Compare this item. Molecular Spectroscopy Consumables & Accessories Thermal Analysis. Robust shaking and consistent temperature control optimize bacteria and yeast cell growth. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life. Answer. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. EMax. Gemini EM Reader scans. Plate types Flat and round 96-well plates Data output Export to PC Detection system Single channel silicon photodiode Reading speed 25 seconds Software SoftMax Pro Software Shaking None Temperature control None Linearity 0. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. No accessories are needed and SoftMax® Pro Software. (96- or 384-well) simultaneously and with high temporal resolution. VIS absorbance detection. These plasticware can come in a variety of formats, such as skirted, semi-skirted, unskirted, clear, colored, and barcoded. Integrated stacker option. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. Contents of wells were pipetted up and down several times to mix. These models can no longer be repaired. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. 190. Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. Values are averages from 5 clean plates, each filled with 200 µL water/well. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. It also integrates with the powerful SoftMax Pro Software for. Selects either 96-well or 384-well plate for a stand-alone read. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. EMax. Packed 25 plates per sleeve, 2 sleeves per case. x. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMolecular Probes® 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and autofluorescence and so are optimized for fluorescence-based applications. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. 2 OD at 200 nm; this decreased to 0. Plus 384. 0, or. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. 039. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. Molecular Devices' microplate readers. The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. 96-well-plate-scan-time-2-colors-1-site-well. Cayman Chemical. 314 low lamp level warning. 0. the use of a reader calibrated with primary NIST and NMI standards. BioTek LogPhase 600 microbiology reader measures microbial growth curves in up to four standard 96-well microplates at a time. 100 fmol luciferase per well. Molecular Devices has provided scientists with . A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. Materials. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. Compare Tool. 7°C and the bottom to 36. No tools required to assemble. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. Step 2: Launch SoftMax Pro Software. FlexIII. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. 2 with 1000 cells. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. Plate and Setup Rack Options Screens with selection of up to four Methods shown. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty EarlyTox Live/Dead Assay Kit (Molecular Devices cat. 5 fg per well to 0. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. g. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Back plate and…. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. 304 signal level saturation. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Contact our support team with your questions regarding Molecular Devices products and applications. 1% in white 96- and < 0. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. Erik Koppes, Richard Haas, Jerry Vockley, Lina Ghaloul-Gonzalez approximately 5,000 cells were seeded in 4 replicates in 96-well plates. xThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Software Validation. SoftMax Pro GxP Software version 5. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. We would like to show you a description here but the site won’t allow us. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. 9. The procedures in this protocol support the 96-well reaction plate. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. 3% in white 96- and 384. 96-, 384-, and 1536-well microplates. Uncontrolled Copy for Training and Reference Purposes Only . The rate of molecular motion of Protein G slows down when it is bound to antibodies, resulting in an increase of the FP value (Figure 2). 5 fg per well to 0. d. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. 0% ± 0. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. A barcode reader accessory can be purchased with theWe would like to show you a description here but the site won’t allow us. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Align the two slots on the bottle holder with the two pegs on the rail. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. 1. 384-WELL SBS STERILE MICROPLATE. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. 2 pM 384–well: 3 pM 96–well: 2 pM 384–well: 4 pM 1 0200-7012POS Glow for 96-, 384-, and 1536-well plates Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA.