LANCE ® Ultra DELFIA ® Certified HTRF Readers and Setups Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. Read & analyse. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Remove the plate sealer and read on an HTRF® compatible reader . White 384-well low volume plate Make sure to use the set-up for Eu3+ Cryptate. 10 μL of GLP1 receptor cells were dispensed into wells of. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. For more information about HTRF. It features a modular design that allows users to add or change detection cartridges according to their needs. FOR HTRF CERTIFIED READER . HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. For more ® information about HTRF compatible readers and for set-up recommendations. Read & analyse. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes• HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Endpoint mode experiments measure each well of the microplate only once. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. FOR HTRF CERTIFIED. Customer Transformation Hub. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. For more information about HTRF ®• HTRF®-Certified Reader. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Read & analyse. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. View Brochure. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Reader. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. Specialized filters allow for IR fluorescence. The plate is set up for reading and images are acquired according to specified parameters. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Simply place the module into one of the five module bays and read the assay. The samples were each placed in a well of a 24-well plate, covered with 1 ml of culture medium, and incubated for a total of 17 days. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. PHERAstar FSX Powerful and most sensitive HTS plate reader . The ratio of the two fluorescence Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. → Incubate 2h (if the detection is not sensitive enough, it is better to incubate 20h). Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Partnerships for delivering turn-key solutions. FOR HTRF CERTIFIED READER. These plates can be read on all HTRF certified readers, thus making their implementation in. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf certifiedLysates are then transferred ®to the assay plate for the detection of Total CHK2 by HTRF reagents. FOR HTRF CERTIFIED READER. The SpectraMax i3x and SpectraMax iD5 readers can be equipped with an HTRF-certified detection cartridge (i3x) or an HTRF-certified module and filters (iD5) to meet the demanding specifications required for HTRF assays. For more information about HTRF. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Provide more sensitive and precise results. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. FOR HTRF CERTIFIED READER. + 4 µL HTRF pre-mixed antibodies . Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Phospho-Rb (Ser780) two-plate assay protocol. FOR HTRF CERTIFIED. n HTRF plate validated (QC with reference HTRF assays) n Low to high volumes (10 µL to 350 µL) n Can be used with all HTRF compatible readers n For cell-based or biochemical assays Trying is Believing! Plate Characteristics HTRF® 96 - well low volume plate CulturPlateTM 96 - well Microplate CulturPlate TM 384 - well Microplate ProxiPlateTM 384 - Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF® 96-well low volume plate TECHNOLOGY ALL THE BENEFITS OF HTRF WITH THE EASE OF USE OF AN ELISA PLATE!. Make sure the setup for Eu3+ Cryptate is used. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a low volume detection plate (either HTRF 384-lv or 96-lv plate) before the addition of HTRF Phospho-Rb (Ser780) detection reagents. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. 1) . HTRF® CertifiedCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. 15 / Each of 1; Qty. A gradient of signal is apparent prior to plate reader optimization (a) causing a %CV of 9. + 4 µL HTRF pre-mixed antibodies. Transcreener® ADP2 FP assay. Alpha Technology: The VICTOR Nivo system. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:320/40 HTRF Eu cryptate 665/7uv Wide Filter 0. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. It can also be further streamlined to a one-. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYDetection. Plate 25 μL of cells in. SV detection plate. The kit includes everything you need: reagents, buffers and a microplate. We would like to show you a description here but the site won’t allow us. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. 96-well culture plateHowever, time-resolved fluorescence (TRF & TR-FRET) assays are used in conjunction with fluorophores that have a longer half-life. The plate was covered both incubated for one hour at room temperature. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. 0. FOR HTRF CERTIFIED READER For more information about HTRF. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed IL1β Antibodies ADD READ ANALYSE Incubate overnight* at room temperature *Reading possibe after 2h White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. 5 nm filter (PerkinElmer 2100-5110) were used. FOR HTRF CERTIFIED READER. • HTRF®-Certified Reader. This protocol gives the cells viability and confluence to be monitored. Dedicated UV-laser excitation. CLARIOstar is equipped with a specific optical device which enables the measurement of. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. In particular, these parameters should be entered as below. Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. Optimization of the reader for the plate dimensions clearly improved the results as seen by the uniform signal (b), a lowered %CV of 2. The optical systems use two scanning All the benefits of HTRF with ELISA plate ease of use. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. The new ligands are divided into four groups according to their chemical structures and/or interactions with YTHDC1 (Table 1 and Fig. Do not pre-mix the d2 and Cryptate solutions prior to dispensing Make sure to use the set-up for Eu Cryptate. VICTOR® Nivo™ multimode plate reader 2 | For research use only. Plate 25 μL of cells in. FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Incubate overnight at RT. The lysis buffer 4X is ready-to-use. How HTRF can simplify your life The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black plates; Dedicated excitation laser and detectors (PMTs) for best sensitivity; Simultaneous detection of donor and acceptor gives speed and precision See full list on bmglabtech. e. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. NC1286225. Sensitive, accurate and reliable detection is critical across cell, protein and genomic analysis applications in drug discovery and development. For more information about HTRF. 25,000 human Jurkat cells were plated in 96 well plates. FOR HTRF CERTIFIED READER. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. The ratio between control lysate signal / non-specific signal should be greater than 2. For more information about HTRF. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. * For HTRF microplate recommendations, please visit . It accepts all types of microtitration plate. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Plate Type and Read Area should be selected based on the microplate used and wells to be read. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Incubate at room temperature. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF Ratio Anti-Tag Antibody Cryptate Inhibitor-Red Inhibitor Purified Tagged Kinase Figure 2: Principle of HTRF kinase competition binding assay (IC 50 - KI determination) HTRF Dasatinib-Red Part # 62KB02REDC & 62KB02REDE Amount: 1 nmol (62KB02REDC) & 20 nmol (62KB02REDE) Concentration: 25 µM in DMSO Form:. COMPONENTS ; STORAGE . HTRF 96 TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: 3h * * Depending on cell lines used, volume of lysis should be optimized. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. Its. For instance, when assay volumes are. Quantify up to 10 analytes per well. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. (In the following text, the term YTHDC1 is used frequently as an abbreviation for the m 6 A-recognition domain of YTHDC1). Incubate overnight at RT. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Plate 25 μL of cells in. For more information. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. For more. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. These plates can be read on all HTRF certified readers, thus making their implementation in. FOR HTRF CERTIFIED READER For more. 4 mg/ml. VICTOR Nivo Multimode Plate Reader. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. This protocol enables the cells' viability and confluence. Catalog No. Read & analyse. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. MUTANT HTT STANDARD WORKING SOLUTIONSLysis buffer 4X for two-plate assay protocol on suspension cells Determine the amount of lysis buffer needed for the experiment. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. 96-well or 384-well small volume (SV) detection microplates - For more information about microplate recommendations, please. HTRF is a proprietary time-resolved fluorescence technology that overcomesThe company offers different HTRF-certified, multidetection microplate readers such as the FLUOstar Omega, POLARstar Omega, CLARIOstar and PHERAstar. LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. + 4 µL HTRF pre-mixed antibodies . FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. Make sure the setup for Eu 3+ Cryptate is used. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. The detection antibody is incubated for 1. Remove the plate sealer and read on an HTRF® compatible reader . No washing steps are required. Nephelometers are dedicated instruments that can only detect scattering and are only available as standalone plate readers. Revvity. In this case the Z´(pronounced Z prime) value comes to mind. FOR HTRF CERTIFIED READER. 5. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Assay plate setup. 96-well culture plateHTRF certified . CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. Customer Transformation Hub. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. At Cisbio we provide microplates that help you discover better. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Catalog No. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. For more. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. 9. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Configure your microplate reader. As shown in Fig. FOR HTRF CERTIFIED READER. Read & analyse. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. + 4 µL HTRF ® pre-mixed antibodies . The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. No washing steps are required. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Lyse cells Read on an HTRF compatible reader . Figure 1: Principle of HTRF Cortisol competitive assay. Cells in each image are identified by SoftMax Pro Software and cell-by-cell HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. 0. Lysates are then transferred to the assay plate for the detection of total-CDK4 by HTRF. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large range of available microplate readers and certainly the detection system already in your lab. These plates can be read on all HTRF certified readers, thus making their implementation in. FOR HTRF CERTIFIED READER. Note that any dilution or improper use of d2 and cryptate Tb-detection reagents will impair the assay quality. For more information about HTRF. It can also be further streamlinedFOR HTRF CERTIFIED READER. -Certified Reader. 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF ® pre-mixed antibodies. Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. For fluorescent assays, black plates absorb light and reduce background and crosstalk. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. FOR HTRF CERTIFIED. Plate 25 μL of cells in. HTRF Microplate Readers. Download Setup. Revvity. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Read & analyse. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. For more information about HTRF ® compatible readers and for set-up recommendations, please. At that stage, the analyte is sandwiched between the 2 antibodies. GENERAL LAB WORK USING HTRF KIT 1 Plate 3 mL of cells in 6-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The kit includes everything you need: reagents, buffers and a microplate. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. This protocol gives the cells viability and confluence to be monitored. FOR HTRF CERTIFIED READER For more information about HTRF. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. FOR HTRF CERTIFIED READER. For a list of HTRF-compatible readers and set-up recommendations, please visit . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. no. PURCHASE SEPARATELY . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. Lysis buffer 1X for two-plate assay protocol on adherent cells Determine the amount of lysis buffer needed for the experiment. VICTOR® Nivo™ multimode plate reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 4. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED. Add. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. S. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. For more information about HTRF. It can also be further streamlined toFOR HTRF CERTIFIED READER. PerkinElmer’s 96w low volume, 96w and 384w microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. For more. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. HTRF is compatible with a large range of available microplate readers. * For HTRF microplate recommendations, please visit . Figure 1. It measures at any wavelength, with top and bottom reading for most standard technologies. Plate 25 μL of cells in. Assay setup for a 384-well low-volume plate. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Make sure the setup for Tb Cryptate is used. FOR HTRF CERTIFIED READER. Microplate readerFluorescence microplate readers usually contain a light source, an optical system for the selection of excitation light (i. HTRF Signal + 5 hours @ RT or . Page 30 4. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more information about set-up and compatible HTRF ® readers,• HTRF®-Certified Reader. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. The VICTOR Nivo is a HTRF certified microplate reader. The CLARIOstar® Plus is our most flexible multi-mode plate reader. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Microplate readers are only certified if they. Learn about the principles, applications, and benefits of HTRF in this Agilent application note. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Measure elevated and normal expression samples without multiple dilutions. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. 47 seconds for a 1536-well plate. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader. Lysates are then transferred to the assay plate for the detection of phospho-c-Src (Tyr419) by HTRF. The PHERAstar ® FSX and CLARIOstar ® microplate readers offer the best possible performance for AlphaScreen ®, AlphaLISA ®, and AlphaPlex™ assays. PerkinElmer. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Seal the plate and incubate 1 hour at RT : Remove the plate sealer and read on an HTRF® compatible reader . The plate was covered and incubated for one hour at room temperature. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. culture plate. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Conclusion. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Revvity. This protocol, HTS designed, allows miniaturization while. and TR-FRET, for example to detect PerkinElmer’s DELFIA, HTRF and LANCE assay technologies Time resolved fluorescence certified for use with HTRF. Neocarzinostatin dose-response on Jurkat cells. FOR HTRF CERTIFIED READER. Plate 25 μL of cells in. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. Example assays are ELISAs, DNA and protein concentration determination, steady-state dose response curves, ADP-Glo™ (Promega), Transcreener™ GDP fluorescent polarization. Preconfigured HTRF. Lysates are then transferred to the assay plate for the detection of total-STING by HTRF. FOR HTRF CERTIFIED READER For. Read & analyse. Incubate 24 hours at RT. FOR HTRF CERTIFIED READER. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. The dual measurement. Unlike standard ELISA plate readers, MSD. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. FOR HTRF CERTIFIED READER For more. Incubate overnight at RT. The optimized well design and high quality plastics provides low background and crosstalk. FOR HTRF CERTIFIED READER For more. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. Make sure the setup for Tb Cryptate is used. Make sure the setup for Eu Cryptate is used. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. protocol with suspension cells or with a. Handle complex biological matrices easily. 3. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. EX: HTRF Emission range. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Due to the chemical and photophysical. Evaluation of the performance of the Spark™ multimode reader for far red fluorescence emission. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate Antibody• HTRF®-Certified Reader. FOR HTRF CERTIFIED READER. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. They can be used in a no-transfer. For more information about HTRF ®Highly compatible plates for HTRF. For more information. An. For more. For more information.