Lamp source is a Xenon flash lamp (1 Joule/flash). The plate reader temperature was set to 37°C and allowed to reach 37°C before proceeding. Uncontrolled Copy for Training and Reference Purposes Only . Laboratory automation workflows can help. S. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. Download PDF versions of the SpectraMax Microplate Reader User Guides. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Data collection speed is sufficient for running fast cell-based kinetic assays. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial. • 0. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Find new and used lab equipment and shop a wide selection of products and vendors. SoftMax® Pro Software Version 5 for Mac and Windows User Guide 5000938 B June 2010 SoftMaxPro_UserGuide_5000938. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. 0200-6263. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes wi. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. Users of Molecular Devices' SpectraMax® i3 Multi-mode reader can even add an optional. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode SpectraMax® Microplate Reader User Manual Download Page. With optimized reagents, option to operate the. Learn how you can utilize microplate readers to drive landmark discoveries. or 0. The plate is set up for reading and images are acquired according to specified parameters. Molecular Devices’ unique SpectraDrop™ Micro-Volume Plate offers a high throughput and accurate solution for low volume measurement. Optimize wavelengths-protocols from Molecular Devices are on computer desktop. 306 plate air cal. Three low volume 24-well bottom slides. Step 4: Incubate at room temperature for 1 hour, protected from light. Turn the reader on and secure the USB cable. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. Step 3: Click “Settings” and a screen will appear (Figure 1). The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. $2,800. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. 2. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. View SoftMax Pro GxP. Page 28 Chapter 5: Read Modes and Read Types 1. An example of an upgradeable multi-mode plate reader is the SpectraMax M5e, a five-mode reader that is part of the same line as the first microplate reader used in space, thanks to its durability. If no sound is heard during initialization, contact technical support. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. 0 . They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Wait 20 seconds. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. SpectraMax Microplate Readers. Data collection speed is sufficient for running fast cell-based kinetic assays. To update the driver. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 312 gain calibration failed. Tests every subsystem of the reader or . Restart the system. Are you looking for a versatile and affordable microplate reader that can perform various assays and measurements? Check out the FilterMax F3 and F5 Multi-Mode Microplate Readers, which offer absorbance, fluorescence, luminescence, and more read modes with high performance and reliability. 0 OD for earlier models. Create custom fluorescence assays without having to purchase expensive filter sets. If no sound is heard during initialization, contact technical support. These tools can be used in conjunction with SoftMax® Pro Software validation package and IQ/OQ/PQ validation protocols for FDA 21 CFR Part 11 compliance. Weight. SoftMax® Pro 7. 1. Some familiarity with Windows should be sufficient to use it. Figure 1. From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. Monday to Friday 7am - 5pm Pacific Time. In the M2, M5 and Plus. Gregory et al. Microplates for Molecular Devices Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Timothy Bolus, MPM, MBA, Compliance Program Manager. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. 5: Schematic of the back panel of a reader. 190. Item SpectraMax i3x Multi-Mode Microplate Reader. 3860 N First Street San Jose, CA 95134. 2. Plate reader instructions Computer. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. SpectraMax® Microplate Readers and SoftMax® Pro Software. Multimode microplate readers offer flexibility, and ease of use. Visible absorbance -. Measure more accurately. These systems are hybrids that allow you to choose between. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Molecular Devices' microplate readers. In . 0A-Sunnyvale, CA-Made in USAMolecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The adapter is required for optimum performance with standard 96-well and 384-. M5. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The advantage of using labeled probes is detection specificity. A plate reader’s software may offer automated analysis using protocols configured for a particular assay. A single software solution addresses your needs, from plate set-up to image analysis. We would like to show you a description here but the site won’t allow us. Explore our fully-integrated,. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Molecular Devices products are warranted to meet the stated specifications. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. US HEADQUARTERS. Select the Driver tab and click Update Driver. Other features and benefits include: Eliminate changing out filters. Plate pre-read measurements were made in a Costar UV plate. Superior optical system includes a xenon flash lamp and features. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. 0) OD (0. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 1700 +1 800. Automated microplate readers reduce manual manipulation and ensure consistent operation. Test up to 3. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. Instrument settings can be saved as a protocol file. For more sample throughput, both readers can be easily. Eight filter options provide simplified assay setup. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. Detector (s) PMT and/or photodiode. 2. Published Dec 16, 2020 | Updated Dec 16, 2020. 2. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. The computer controlling the plate reader runs Windows XP. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 0 × 107) RLU 0. ” So, acquisition and analysis come from one software system. Recombinant protein expression Nucleocapsid. Luminescence is the emission of light by a substance as a result of a chemical reaction (chemiluminescence) or an enzymatic reaction (bioluminescence). 8×15×8. Maintaining data integrity is a. This can increase throughput and efficiency, thus saving time and space in the lab. Contact our support team with your questions regarding Molecular Devices products and applications. These applications may make use of…FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. Description 1. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Versa Max. 340 PC384. S. Read plate standard and mix. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. It has dual scanning monochromators that allow for 1 nm increment selection over the wavelength range of 250 - 850 nm. Important things to consider when purchasing a multimode microplate reader. Find regional support. SpectraMax Absorbance, Fluorescence. gene expression with a user-friendly reader. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. 0 . Also refer to the Multimode Reader Comparison Chart Features. Compact designs. This can increase throughput and efficiency, thus saving time and space in the lab. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. com 4. Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast,. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. integration time per well, depending on firmware version. 400×320×350 mm. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Plate reader. Microplate Reader, Multimode. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. For more sample throughput, the system can be easily integrated into a full roboticOnly Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. One plate carrier adapter is provided with the instrument. Molecular Devices, LLC. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. ~ Bio-Tek ELx808 Microplate Reader $2,795. FLIPR. Fortunately, Molecular Devices’ SpectraMax i3x and iD5 readers offer the best of both worlds. 25 to 3. A). Multimode Microplate Readers. To update the driver. Experimental details and customization of the group table. An ampicillin-resistant E. Successful targets include calcium channels, chemokine receptors, and transiently transfected receptors. Absorbance Plate Reader Comparison Chart. Methods. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Multi-mode Plate Reader. FLIPR. 6 kg (30 lbs. The cuvette must be in place when you read the plate. 5 sec. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Chapter 1: Introduction The SpectraMax® iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. The SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplateMicroplate Spectrophotometers. Read height should be optimized for Vmax® plate reader specifications. Ensure data accuracy of your reader. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Plate stacker and robot integration The SpectraMax 340PC384 reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. Fixed cells stained with fluoresceinconjugated anti-VCAM are identified with the Marker Expression Protocol. The adapter is required for optimum performance with standard 96-well and 384-. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Microplate Reader, Multimode Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. Molecular Devices' microplate readers. 9000-0756. +44-118-944-8000. is user upgradeable—from 2 to 3 modes, providing researchers with a versatile instrument that does not compromise on . The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in writing. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Molecular Devices' microplate readers. SoftMax Pro 7. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. 5. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 18 kg. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. With SoftMax Pro Software, the PathCheck Sensor automatically normalizesThe SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 00. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 1. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. absorbance plate readers. The reader measures both flat and round 96-well microplates and. 2. Search. Temperature Control 4°C above ambient to 45°C. First, as mentioned before, the walkaway time significantly. These lab workhorses are used in drug discovery. The plate reader optics are tunable for absorbance. Easy-to-use touchscreen allows you to quickly set up your experiments, use preconfigured analysis methods, and view tutorial videos. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. , target CV per well = 1. Endpoint. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. Imaging System Giles Scientific Biomic V3 Automatic Plate. Select Start>Control panel>Device Manager. Find our technical support information along with contact details. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. It is more inert and reduces compound interference. July 17, 2023. The touchscreen interface provides integrated instrument control, data display, and the ability to export results for statistical data analysis. Plate Reader-2 features a wide range of high-performance multi-mode reader capabilities ideal for life science research on the International Space Station (ISS. 9000-0756. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. The former have been the traditional domain of plate readers and great strides have been. To purchase a newer software version,. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Lab automation for high-throughput plate-based assays. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. The plate is set up for reading and images are acquired according to specified parameters. Molecular Devices SoftMax ® Pro software was used to analyze and present the data. 2. Fluorescence analysis was performed using a SpectraMax iD3 multi-mode plate reader (Molecular Devices LLC, U. Versa. The plate was then re-read on the plate reader (Read B). Step 2: Launch SoftMax Pro Software. Molecular Devices’ SpectraDrop TM MicroVolume Microplate for SpectraMax ® microplate readers offers higher throughput than any other low-volume microplate, with up to 64 samples per plate. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. In this product video, see how the SpectraMax M5 quickly reads samples. BMG Labtech - THERMOstar. Superior optics Microplate Reader Shelf. Step 2: Add sample to well – antigen within the sample binds to the capture antibody. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. #0200-7011) Instrument setup SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Perform plate-based assays on a large number of plates without having to manually feed them into your microplate washer and reader. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Title: F3/F5 Multi Mode Microplate Readers | Molecular Devices Author: Molecular Devices, LLC Subject: Learn how the F3/F5 Multi-mode microplate readers can utilize its filter sets to accurately perform measurements with up to five modes of detection. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A)Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. When the user applies a set temperature of 37°C, the top is heated to 37. For nearly 30 years, Molecular Devices has provided scientists with . Step 1: Turn on your microplate reader. +1 800-635-5577. The SpectraMax 190 reader can be integrated with Molecular . This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. This reader's dual monochromators for. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. 1. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. The adapter is required for optimum performa nce with standard 96-well and 384-. begin reading microplates with seven mouse clicks. Cellular. . The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The Bauer Core Facility at Harvard University offers of variety of plate-reader choices to its users (mostly from Molecular Devices), and the SpectraMax® L is preferred for luminescence rather than the core’s multimode readers. 6 % of reading 4. The opaque walls prevent stray light from being detected from neighbouring. The content of this site is published by the site. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Turn on Plate Reader and wait for it to complete its start-up routine. PURPOSE. 0 . The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. If the computer is connected to the Internet: Select Search Automatically for. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. 9000-0756. Recertification service. After reading the ELISA plate on both readers, each standard curve was nearly identical. The plate reader. HTRF-compatible SpectraMax Multi-Mode Readers. The FlexStation uses a Xenon flashlamp and monochromators for both excitation and emission light to allow the use of a wide range of. 1 up to 3600 sec. M2. the use of a reader calibrated with primary NIST and NMI standards. The SpectraMax M2 and M2e are versatile and reliable. Built-in near-field communication (NFC) reader enables you to pull up your custom protocols and results with a single tap, saving you precious time. Wait 20 seconds. Never order or replace filters to optimize an assay again!SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. With the discontinuous kinetics feature in SoftMax Pro Software. FAST AND ACCURATE METERTECH Microplate Reader $3,650. It consists of a SpectraMax® Microplate Reader, Peak Robotics S-LAB Plate Handler with the S-LAB software, and the AquaMax® 4000 Microplate Washer. 40266 Email: dru[email protected] iD5 reader’s filter system. READ ANALYZE IMPORTa single plate read using any standard cuvette, or 96- or 384-well microplate. W×D×H. Molecular Devices, LLC. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. With optimized reagents, option to operate the readers. S. in steps of 0. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. Figure 1. Samples with volumes as low as 2 µL can be measured. Evaluation of BioTek's Synergy H4 Plate Reader for Fluorescent Detection. The most published microplate reader control and data analysis software. For biochemical assays, the washer removes unbound material and unreacted reagents. FLIPR Calcium 4 Assay Kit. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. Latest Citations: For a complete list, please click here. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. 5 hours. Endpoint, kinetic, spectrum. Description Figure 1. Reader Documentation Version No. Restart the system. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. D. SoftMax Pro 7. 1. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. Feb 22, 2023The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular.