using SpectraMax i3 (Molecular devices, San Jose, USA). SpectraMax Injector Cartridge with SmartInject™ Technology. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). Tunable for filterless flexibility. 5. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 314 low lamp level warning. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). Following HRP-conjugated secondary antibodies, goat anti-rat IgG (Kirkegaard & Perry. , Sunnyvale, CA). Service parts for these models are not being produced and are no longer in stock. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. SpectraMax Multi-Mode Microplate Readers. The microtiter plate was then incubated in the dark for 2 h at 37°C. SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Or scan up to 384 wells at any time to determine the best assay conditions. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). S. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 04. Multi-mode Microplate Readers. Details. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). 2. One plate carrier adapter is provided with the instrument. The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. or microplate reader (e. Details. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. N-34-LB. . Absorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). MMP‑1 inhibition assay. A. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. addresses the needs of life science researchers who quantify very small amounts of DNA, RNA, oligos, and proteins. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. SpectraMax Multi-Mode Microplate Readers. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). 305 reference level saturation. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Microplate Readers. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. Statistical analysis. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). 5% (w/v) sodium dodecyl sulfate-polyacrylamide gels, and transferred to immobilon polyvinylidenedifluoride membranes (Millipore, Bedford, USA). WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. 5), L-ascorbic acid (25. used. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). GE Healthcare spectramax 340 pc plate reader Spectramax 340 Pc Plate Reader, supplied by GE Healthcare, used in various techniques. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. 8. SpectraMax Multi-Mode Microplate Readers. The light features a slim profile to prevent. Products : SpectraMax Plus 384 reader,. Call 1-800-745-2710. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). This assay measures the two major sugars in wine, glucose and fructose. ac. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Softmax PRO v5. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. Data were expressed as the mean LPS-treated cells (100%) vs. Each experiment was performed in duplicate and repeated at. The plate was covered and incubated for one hour at room temperature. , Sunnyvale, CA). SpectraMax Multi-Mode Microplate Readers. D2. Wavelength Range. . Molecular Devices SpectraMax 340 PC 384 Microplate Reader. SpectraMax Microplate Readers. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. MCE has not independently confirmed the accuracy of these methods. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The loading equaled the mass of BSA per mass of particles. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 00 USD. Photometric Performance SpectraMax 340PC384 Item Description Wavelength range 340 nm to 850 nm Wavelength selection Monochromator tunable in 1 nm increments Wavelength bandwidth. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. Molecular Devices Corporation 1311. Incubation with TMB substrate was done for 18 min. Customer Transformation Hub. Absorbance was measured at 490/650 nm by using a SpectraMax 340 microplate spectrophotometer® (Molecular Devices, Sunnyvale, CA, U. 312 gain calibration failed. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. 5. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. RESEARCH ARTICLE Effects of early-life conditions on innate immune function in adult zebra finches Merijn M. The adapter is. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. The absorbances were being measured at 515 nm. Also for: Spectramax 190, Spectramax 340pc384, Versamax. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. 00. 10. Amino Acid Analysis. Briefly, cells were seeded into a. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. Details. 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. Exhibit with us {{>currencyLabel}} Back. 304 signal level saturation. One plate carrier adapter is provided with the instrument. albicans pre. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. 1x10 5 transduced, GFP + SK-NEP-1. Isolation of RNA and RT-PCR. Molecular Devices SpectraMax M5e Microplate Reader $26,500. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. Details. 306 plate air cal fail,low light. Both readers feature an 8-channel read head for fast reads and. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. Gerritsma,B. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. SpectraMAX 340 microtiter plate reader. 306 plate air cal. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. The cell proliferation on different Ti discs was assessed by measuring the absorbance at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Devices, Sunnyvale, CA). 2. Details. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Manager: Yinghua Chen, PhD Contact Email: yinghua. TDP-43 stress granular cells were treated with 70% ice-cold. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The sensitivities of the hormones are Prolactin: 0. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. 6) and 0. The slope of the linear portion of the kinetic curve generated from each well was used to. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. Stat Fax 3200 Microplate Reader. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. Crowder, Ziqian Zeng, Alissa N. Fully tested with Warranty. The membranes were. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Money Saved: $340. The intensity of red color formed in the assay was measured at a wavelength of 490 nm with an ELISA reader (SpectraMax 340, Molecular Device Co. Assay setup for a 384-well low-volume plate. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. Nitrocefin hydrolysis was monitored at 482 nm on a SpectraMax 340 UV-visible plate reader for high-throughput assays and an HP8453 UV-visible spectrophotometer for the low-throughput assays. P. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. used in the SPECTRAmax PLUS. It includes both a lamp and a new lamp holder. control. The OD 490 was measured with an absorbance microplate reader (SpectraMax 340; Molecular Devices). They are for reference only. The SPECTRAmax 340PC can measure. chen@case. The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. TGFα release was calculated as described in the Results. plates can be used in the SPECTRAmax PLUS 384. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. A 0. The reaction was stopped with 1 M hydrochloric acid after 15 min. Stat Fax 4700 Microstrip Reader. Molecular Devices SpectraMax M5e Microplate Reader. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Warranty. SPECTRAmax® 190. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 5. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). The initial rate of hydrolysis was determined using the early linear region of. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Manager: Yinghua Chen, PhD Contact Email: yinghua. StakMax. The SPECTRAmax 340PC can measure. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. Other technologies such as imaging. 304 signal level saturation. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 2. 85%. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Operators can conveniently select up to 6 wavelengths at a time for. Molecular Devices SPECTRAmax. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. SpectraMax Plus 384 measuring instruments pdf manual download. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hIranian Journal of Basic Medical Sciences ijbms. Italic and boldface type are used for emphasis. 1. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Subsequent replacements require only the lamp (N-34-LB) be replaced. 2. This is equivalent to having 511 built-in filters,Colour change was measured in a SpectraMAX ® 340 spectrophotometer. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. 001/min at 425 nm. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The Catalina. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . Western blot. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Molecular Devices Spectramax 340 Plate Reader Repair. Analysis of ALA by Ehrlich’s Reagent Assay. Changes in OD 405 nm were followed on the SpectraMAX 340 plate reader. Donor filter: 620 nm. Includes: SpectraMax 340PC384. Bertin Precellys Evolution Touch Homogenizer. SoftMax Pro. Optical density was measured using a SpectraMAX 340 (Molecular Devices, Sunnyvale, CA). CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. SpectraMax Multi-Mode Microplate Readers. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Water – distilled water or equivalent. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andSPECTRAmax ® PLUS 384 Microplate Spectrophotometer. chen@case. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. 1 0 100 80 60 40 20 0 4. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. See More Microplate Reader / Microtiter Plate Reader. Although all samples initially were analyzed at the same volume, the volume of sample. 20. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Labsystems Luminoskan RT Microplate Reader. PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. Cell proliferation was measured using the MTT method. Briefly, ear or dorsal skin was dissected and fixed with 10% phosphate buffered formalin for 24–72 h. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). 2 - 3. Male 8 pin mini Din to Female 9D. FlexStation® 3 Multi-Mode Microplate Reader. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive. Briefly, cells were seeded into a. These lab workhorses are used in drug discovery, research, bioassay validation, and. Product Keyword. 1 mL of acetylacetone. DNA extraction from blood meal ELISA homogenate. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Up for your consideration is a Molecular Devices SpectraMax 340 Microplate Spectrophotometer Model# 340 Condition is for parts or repair. Samples were considered “positive” if the absorbance value was greater than two times the average absorbance value of the negative control wells . Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. Fully tested with WarrantyThe SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Plates coated with no antigen, canine CD19, streptavidin alone, and streptavidin with irrelevant human avitag-CD3ε (Acro Biosystems, Newark, DE) were used as negative controls. NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Percent viability was calculated by comparison with the control group. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. After adding sample, the mixture was incubated for 30 min at 37°C, with one unit of enzymatic activity being defined as an increase in absorbance of 0. SpectraMax 340 (Obsolete), SpectraMax 340PC 384 (Obsolete), Gemini EM reader, SpectraMax 250 (Obsolete) What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full. Examples: “Press carefully to engage,” “Do not press down. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that. No power cord or accessories included. (1993). The plates were incubated for 4 additional hours at 37°C. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). (1988) and recorded at 412 nm. They are for reference only. Description 1. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). SpectraMAX 340 plate reader (Molecular Devices). 0 1. The absorbance at 405 nm was determined using a microplate reader (spectraMAX 340). Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. SpectraMax 340 Absorbance Microplate Reader, Molecular Devices (CAT#: STEM-SPO-0021-ZJF) Labsystems Fluoroskan II Fluorescent Microplate Reader, Excitation Wavelength Range 320 – 700 nm, Labsystems (CAT#: STEM-SPO-0006-LGZ) BioTek MQX200 Microplate Reader Spectrophotometer, 24VDC, BioTek Instruments, Inc (CAT#: STEM. This reader only allows high performance HTRF measurement when assays are run in. #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. TGFα release was calculated as described in the Results. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- pathways and protein activation and expression in one system. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. 5 stars - 1602 reviewsThis assay typically develops in 1. 340 nm / 80 nm. -1 activity was determined MMP using an MMP-1 immunoassay kit (dY901; R&d Systems, Inc. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. 313 reference gain check fail. The Spectra Catalina 340 Automated Connect 12Volt or 24Volt Catalina 340C produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize. Microplate Readers. The reaction mixture was incubated for 5 min. Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). g. Upgradeable modules are also available including western blot, cell imaging, and fast. It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 0. The. 0 OD. Each experiment was performed in duplicate and repeated at. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Each SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. SpectraMax Microplate Readers. using a Molecular Dynamics Model SpectraMAX 340 PC microplate reader. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). SpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. VersaMax reads 96-well and strip-well plates. Briefly, cells were sequentially treated with terminal deoxynucleotidyl. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. HTRF-compatible SpectraMax Multi-Mode Readers. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Optical density was read at 405 nm after 30 min using a Molecular Devices SpectraMax 340 spectrophotometer. Description 1. In this study we used tetanus toxoid (TT) as a well-defined antigen to. 3. 2. Donor: 616/10 nm. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Molecular Devices’ PathCheck® sensor technology. All assays were performed using a sample size of 12, and the absorbance at 425 nm was measured by using a SpectraMax 340 multiwell plate reader (Molecular Devices, Sunnyvale, CA). About GMI provides new and GMI Certified Pre-Owned scientific instrumentation and service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics and analytical laboratories, in the United. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. 4) was followed according to Vanacker et al. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). 190-1000 nm, tunable in 1 nm increments. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA).