The user guide also covers the features and specifications of the SpectraMax MiniMax 300. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. In order to calculate a humification index (HIX), spectra were obtained with an excitation wavelength of 254 nm over the emissions waveband of 300–480 nm at increments of 5 nm (Ohno 2002. Description 1. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Shaking and incubations were performed using an on-deck orbital shaker. Unlimited breadth of. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. Find regional support. . Supported Windows Operating System: See release notes. . SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 2 x 10-14. The calculated LOQ values were 0. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . 1. The dual auto injector module. SpectraMax Microplate Readers. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Figure 1. to View Figures. Curves were forced through x,y = 0. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. , Fisher Scienti fi c, cat. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. Find regional support. Microplate Reader Shelf. Nucleic Acid and Protein Quantitation. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. SpectraMax Paradigm Cisbio HTRF Detection Cartridge User Guide 5008539 A 5 Laser Light Laser Specifications The SpectraMax® Paradigm® Multi-Mode Detection Platform is rated a Class 1 Laser Product because it houses one or more Laser Modules whereas the laser light is not accessible. 58% for the detection module and 2. The calculated LOQ values were 0. compound concentration, and an IC 50 value of 21. Bioz Stars score: 98/100, based on 1 PubMed citations. 6% for the on-board optics (monochromators). Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. com. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. applications, including dual luciferase and ATP assays. Shake intensity. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when. Supplier: Molecular Devices. 2 or higher,. The corrected absorbance is within 5% of the. Facilities. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viability Introduction. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. Download PDF. Image analysis provides accurate data. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax iD3 and iD5 Register or Sign In. 1 and above: Compatible with only with 64-bit OS. Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. Learn more about its features, specifications, and applications in this user guide. Prepare your i3/i3x instrument for shipment. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . 0´ 104 1. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. HTRF IP standard curve. Find regional support. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. Italic and boldface type are used for emphasis. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThis characterization of nanoparticle and its surface interactions can be detected using the SpectraMax i3x Multi-Mode Microplate Reader and the Spectral Optimization Wizard in SoftMax Pro Software. Spectranet customer portal. 0. The information is below the horizontal line. The. Spectranet customer portalThe SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. 3. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 0´ 104 8. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. . Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. *On the SpectraMax i3x reader only. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. 1. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. GloMax Discover System. 800. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. GM3000 Register or Sign In. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . Multimode Readers Comparison Chart. 1 and above:. GFP expression was higher at 21 hours post transfection than at 16 hours (16-hour data not shown). With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax. 58% for the detection module and 2. 01 0. 4. Published Dec 16, 2020 | Updated Oct 10, 2023. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. iD5. 020 fmol 0. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 2. Register to download your free eBook today. 99. Note: Additional readers with similar performance are listed. 314 low lamp level warning. Viewing this answer requires an authorized account. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideRecommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. HTRF IP standard curve. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Expanded dynamic range Engineered for performance with spectral fusion illumination for increased sensitivity across the entire excitation range. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. View the Molecular Devices website. i3x Multi-Mode Microplate Reader. using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. We would like to show you a description here but the site won’t allow us. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 304 signal level saturation. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. Find regional support. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Introduction. The SpectraMax i3x Multi-Mode Microplate Reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Both readers demonstrated their ability to detect the HTRF signal from the terbium donor and red acceptor combination, and they could be used for saturation studies using the Tag-lite technology. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. 5. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. . Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. i3x single use: $35: $52. . 98 / 100 stars. detection cartridge LUM96: 59. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellEvery SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. Average 98 stars, based on 1 article reviews. . This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. . Spectranet customer portal(SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. no. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Absorbance Fluorescence (top/bottom read) Luminescence (top read) Time-Resolved Fluorescence (top read). HTRF-compatible SpectraMax Multi-Mode Readers. Data analysis. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. SpectraMax i3x Multi-Mode Microplate Reader. As. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. Homogeneous and eterogeneous microplate assays can be performed in one flexible system. Unplug the power cable from the back of the instrument. StakMax Handling Stacker Base Unit. 3 STANDARDIZE TO A SINGLE ANALYSIS PLATFORMLearn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax® i3x injector module with SmartInject® Technology ensuring equal mixing across the plate for high-precision experiments. Multimode Reader Comparison Chart. Settings not required by a reader are indicated by ‘---’. Explore a wealth of applications with the future-ready SpectraMax i3x. *On the SpectraMax i3x reader only. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. 因为是导师第一任学生，没有师兄师姐，加上本人悟性也差，小小pcr花了半年时间才终于有点样子。因为经历过，所以懂得，故而分享之，希望能给到屏幕前的你一点点帮助! 如有失误，欢迎指正~最后，不要忘了一键三联哟，谢谢大家！, 视频播放量 6293、弹幕量 2、点赞数 122、投硬币枚数 55、收藏. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 10. The protocols are also available on the SoftMax Pro Software protocol sharing. Keywords: htrf spectramax detection cartridge, spectramax i3, spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. Manufacturers try to design their instruments so they can be. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. Examples: “Press carefully to engage,” “ Do not. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraMax i3x reader provides the The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 9000-0756. Negative samples contained only MycoAlert Assay Buffer. 価格、見積もり、お近くの販売店を. 0. 22mm 384 FilterMax™F3&F5Multi-ModeMicroplateReaders,SpectraMax®i3x Multi-ModeDetectionPlatform,SpectraMax®iD3&iD5Multi-Mode MicroplateReaders,andSpectraMax®Paradigm®Multi-Mode MicroplateReader 5301193 18mm 96 SpectraMaxi3x,SpectraMaxiD3,SpectraMaxiD5,SpectraMaxM. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. ZERO BIAS - scores, article reviews, protocol conditions and more. For each kit, the reader was able to detect the lowest dilution as positive. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformVersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. The treatments were. 58% for the detection module and 2. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Subsequently, 100 μL of the substrate solution (2 mM of p-nitrophenyl butyrate in dimethylformamide) was added into the mixture, and incubated at 37°C for 15 min. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. 4. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析 We would like to show you a description here but the site won’t allow us. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and. The dynamic range of the assay is about 3. SpectraMax i3x and Paradigm readers require the FP-FLUO. o For example: Ex: 485/Em 525/Cutoff 515 4. 1. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. SpectraMaxi3Multi. Recommended settings for SpectraMax iD5 and i3x readers. were measured at OD405 using a SpectraMax i3x multimode detection system (Molecular Devices, San Jose, CA, USA), and the melanin concentration was determined using the standard curve of melanin solutions with known concentrations. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataSpectraMax i3x Multi-Mode Microplate Reader. 더 많은. ValitaTiter Plus settingsContact our support team with your questions regarding Molecular Devices products and applications. 1-xiAt last, the relative absorption was estimated using a microplate reader (SpectraMax i3x, Molecular Devices, LLC, Sunnyvale, CA, USA) at 450 nm. QuickSync. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. 0 mm hex key. Figure 4. Figure 3. Unparalleled performance on a personalized platform. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Time-. +44 118 944 8000. (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. 0. The SpectraMax i3x reader provides theThe SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. pathways and protein activation and expression in one system. Bulk Kit. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. ü; ü. 3) At the bottom of the strip, click on "Show. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. Actual shake speed is based on the microplate format. Find regional support. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. 5 µL anti-cAMP-Cryptate. It can be upgraded with. . We would like to show you a description here but the site won’t allow us. See Unpacking the SpectraMax Paradigm Instrument on page 1. Products : SpectraMax i3x reader, SoftMax Pro GxP Software, SpectraMax i3, SoftMax Pro Software, SpectraMax MiniMax cytometer When I attempt a read with my SpectraMax i3 or i3x reader using the injector cartridge, why do I see a plate height error, even though I have entered the correct plate height?. 00800 665 32860. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. . . . In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. Determination of anti-inflammatory. Cell counters for accurate and. 6% for the on-board optics (monochromators). The dual auto injector module. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Automated for ease of use. . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 100 fmol 384-well (75 µL) 0. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Fluorescent Polarization: Solid black plates, as above. Unlimited breadth of. Explore Multi-Mode Validation Plate that confirms instrument performance for spectramax i3, i3x, Paradigm system & FilterMax Reader. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. SpectraMax M5. • Removing the transport locks from the PMT shutters and drawers. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). ; The top 3 importers of Spectramax are India with 365. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. 在这里，我们展示了如何使用SpectraMax®i3x和 SpectraMax®iD5多功能微孔板读板机应用HTRF技术进行 Gq-偶联受体的分析。G蛋白偶联受体的分析主要通过检测 cAMP和IP3这两条信号转导通路调控介导的细胞内钙离子水 平的升高。Cisbio公司的IP-One Gq试剂盒通过检. Summary of Contents for Molecular Devices SpectraMax M2. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SpectraDrop Abs Protein Quant (i3-i3x)Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. i3. Wokingham, Berkshire RG41 5TS United Kingdom. Register to download your free eBook today. 5 Figures from 202 Publications Thermo Fisher Scientific. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. After 2 days of incubation, LUC and REN activities were measured using a SpectraMax ® i3x Multi-Mode detection platform (Molecular Devices, USA) with a Dual-Luciferase Reporter Assay Kit (Promega. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Supplier: Molecular Devices. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 306 plate air cal fail,low light. 635. Quantiferon-TB Gold in Tube Kit. com. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Average 98 stars, based on 1 article reviews. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,(foc) spectramax i3x alphascreen 384 std detection cartridge - p/no:0200-7017 (cartridge for demonstration purpose) united kingdom: chennai air cargo: unt: 1: 1,254,478: 1,254,478: aug 04 2016: 90273090: spectramax i3x multi-mode microplate reader; iqoq for spectramax i3x smp7 gxp license(for r&d lab use only) united states: bombay air cargo. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Figure 2-1 Label on Rear of Instrument WARNING!We would like to show you a description here but the site won’t allow us. Instrument. Simplified workflow: Add cellular imaging assays in a small footprint. Danaher Inc spectramax i3x. Bioz Stars score: 98/100, based on 1 PubMed citations. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringLuciferase activity was measured using a SpectraMax i3x microplate reader (Molecular Devices). SpectraMax i3x readers. *On the SpectraMax i3x reader only. 303 unable to cal dark current. Spectranet customer portalSpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. SpectraMax® ABS and ABS Plus reader support; ü. Molecular Devices. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. . Table 1. ZERO BIAS - scores, article reviews, protocol conditions and more. Tristar 5 Multimode Microplate Reader. For more sample throughput, the system canMicroplates were sealed to reduce evaporation (adhesive PCR plate seal; Thermo Fisher Scientific, AB0558) and placed in a microplate reader (Molecular Devices, SpectraMax i3x) for 8 h at 30 °C. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 5mM EDTA, 1% NP40,Explore a wealth of applications with the future-ready SpectraMax i3x. g. Eliminate time-dependent substrate addition steps. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Engineered for high performance, the auto dual injector cartridge includes fail safe features. The SpectraMax M4 reader had identical performance specifications to the SpectraMaxLuminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. More Product Information. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). It allows you to explore cellular pathways and protein activation and expression in one system. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringfluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiterA microplate reader that can detect two or more applications is considered a multi-mode plate reader. The chamber. . SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 99. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Nuclear Receptor. . . ü; SpectraMax® Mini reader support. SoftMax Pro User’s Manual v Contents Figures. It allows you to explore cellular pathways and protein activation and expression in one system. Read modes. 1 Microplate Data Acquisition & Analysis Software for Windows. Reaction catalyzed by the firefly luciferase. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Description 1. 313 reference gain check fail.