This can increase throughput and efficiency, thus saving time and space in the lab. 67. Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. Description 1. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. 60 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. We here describe a fluorescence resonance energy transfer (FRET) based fluorescence plate reader assay to monitor the interaction between fluorescently tagged sortilin and p75 NTR in live cells. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 1 x 38. Published Dec 16, 2020 | Updated Dec 16, 2020. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. 209, but in 96-well plate was 0. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The SpectraMax iD3 reader detects fluorescence using monochromators only. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. com Setup for Omnia® assays on SpectraMax®. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Automated live cell imaging – cell counting and confluence. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance. td. The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Align the two slots on the bottle holder with the two pegs on the rail. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. White plates reflect the light, enabling even low levels of Luminescence to be detected. 1) A maximal fluorescence intensity of 177 ± 3 RFU was observed at the highest cell concentration (Table 1). The SpectraMax Plus 384 Microplate Reader for. dual monochRomatoRs foR assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. 00 CAD. Dose-dependent enzyme essayVisualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. It may be named "Pothos". In addition, the SpectraMax iD5SpectraMax Microplate Readers. 0 out of 5 $ 14,499 $ 13,995-13%. SpectraMax ® M2 Multi-Mode Microplate Reader. Other technologies such as imaging. Using standard fluorescence optimization can take hours or days to determine the best wavelengths to use for excitation. When displaced, changes in fluorescence can be read in an FP, FI, or TR-FRET depending on the assay format. FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. I've got a LT4500 microplate reader and was hoping to digest the pellets in papain overnight, simply mix a measure of that sample with DMMB solution in a 96 well plate and read absorbance at 525nm. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. as a fluorescence blank. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. 314 low lamp level warning. The flexibility of a high end multimode plate reader with environmental control. Compatible automation solutions for SpectraMax Readers. SpectraMax M2. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. In simplest terms, a plate reader measures and records a signal produced from the wells of a multiwell plate. Fluorescence measurements no longer require you to adjust the gain. To schedule a training session please contact Ewa Folta-Stogniew. The University of Rouen. 04 to 3. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. By employing dyes that specifically bind to nucleic acids, DNA/RNA concentrations can also be quantified using fluorescence detection. Offer. 0 out of 5 $ 9,995 $ 9,495. PC software (SkanIt software) Dimensions (D x W x H) 58 x 53 x 51 cm (23 x 21 x 20 in) Weight. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Figure 2-23: Bottle holder To install the bottle holder: 1. Customer Satisfaction is. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. SpectraMax M2E Multimode plate reader with cuvette option, ABS and FLR reading and extended range. Perkin Elmer EnVision 2104 Multilabel Plate Reader, Perkin Elmer, Envision Plate Reader. fluorescence intensity (Figure 6). For paper-based measurements, we used the Milenia HybriDetect 1 (TwistDx) lateral flow system, as per the manufacturer's. D 3 1. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Multimode microplate readers offer flexibility, and ease of use. Peeking into a research lab today, it would be rare to see a line-up of microplate readers on a bench, each capable of performing only a single type of measurement. Perform the scan and note the wavelength that gives maximum emission. It also. 6 % of reading 4. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). SpectraMax Paradigm SpectraMax i3 SpectraMax Flex-Station 3 SpectraMax F5 SpectraMax M4, M5, and M5e SpectraMax. CA$14,500. 00. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. It can measure absorbance from 230 -1000 nm. Shop now. 4. Refurbished. The software is easy to use and contains settings for hundreds of commonly used assays, such as BCA and MTT. The plate blank’s RFU values should be subtracted from the RFUs of all samples of the plate. Category 2 and 3 microplate readers use gradient heating as well. FAST AND ACCURATE METERTECH Microplate Reader $3,650. 0) OD (500 to 1100) RFU (5. 2. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. A microplate reader as individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). The spectrophotometric performance of the SpectraMax M2 and SpectraMax M2e is similar to the SpectraMax Plus, a dedicated absorbance plate reader. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck® Sensor. 5%in96-and384-wellmicroplates. (in addition to fluorescence imaging) on the SpectraMax i3x Multi-Mode Microplate Reader. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. Biotek FLx800 Fluorescence Microplate Reader. The entry should be SpectraMax GEMINI EM. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. FI assay. Absorbance (monochromator) Fluorescence (monochromator; FI, TR, FP) Luminescence; Molecular Devices Spectramax M2. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Fluorescence Microplate Reader Comparison Chart. Microplate Reader Shelf. Data fromSpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E. NanoQuant plate. 627. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. Incubation. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. New Life Scientific backs all orders with a 90-day warranty. The cooled PMT detector allows high sensitivity in a wide dynamic range, even in extremely low light. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. Optimizing Time-Resolved Fluorescence Read Mode The SpectraMax M2 enables you to read Time-Resolved Fluorescence assays from the top of a plate. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. $ 9,499. Plate background OD values of disposable UV microplates vary slightly between lots. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. This enables the standard curve to be plotted and sample concentrations to be calculated automatically by SoftMax. Appendix. Top detection is available for fluorescence detection on the SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2e. 5. Add to Cart. TRF as Secondary Detection Mode: TRF is supported as a secondary detection mode by these readers: Gemini XS, EM, XPS, M2, & FlexStation. 8 Customer Reviews. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. BMG Labtech - THERMOstar. Last lab had ours for years and never had a problem with it. 1. The plate reader optics are tunable for absorbance. The SpectraMax. Plate chamber design of the SpectraMax category 1 microplate readers. 3) At the bottom of the strip, click on "Show. The chamber. ) Identical data and curves were generated from Gemini EM and SpectraMax M2 but are not shown here. $16,000. the reader’s stated range. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Microplate Readers. NOTE: This assay differs from the previous one in that the fluorescent dye ThT or ThS is included in the aggregation reaction. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Wide range of applications The VersaMax reader covers a wide range of applications:Molecular Devices SpectraMax M2 Multimode Plate Reader. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader andMicroplate Readers. . A whole well fluorescence imaging system that can collect information from all cells inside the well, from 6- to 384-well formats. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Description 1. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The analysis capabilities of SoftMax Pro Software provide quantitation in an easy-to-read, user-customizable report format. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. WinKQCL ® Software can be used with a compatible microplate reader to run the template and. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers are as Fluorescence Validation Test Plates provide validation of the system’s optical characteristics. Bottom Monochromator: Fluorescein 2. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). spr * SpectraMax i3(x) SpectraTest ABS2 v1. 313 reference gain check fail. Five preferred detection modes. 7. 1. Microplate Readers. 00. Search: Submit search Search filters Search This site Search Cornell. 40266 Email: drugdiscoverytech@lifetech. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax ® Pro. I am wondering if the type of 96-well plate matters. D 59 A. BioTek Synergy HTX Microplate Reader - Multi Mode (Abs + FL + Lum), Bio-tek, Used plate reader, BioTek multi-mode detection, PMT for luminescence detection, kinetic assays, Time Resolved Fluorescence, Gen5 software, absorbance reader, fluorescence detection, used lab. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. a multi-detection microplate reader with two-mode cuvette port Spectramax® m2m2e user guide SpectraMax M2e user guide cover 1 1 42106 9:53:28 AM SpectraMax M2 SpectraMax…Spectramax i3 Plate Reader. The fluorescence was measured in the SpectraMax M3 Microplate reader. 7°C (gradient heating design). The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. 7°C (gradient heating design). Wavelength optimization. The SpectraMax microplate readers support a wide variety of assay categories including . Optical System: High energy xenon flash lamp. SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. Product Information. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. The fluorometric. A). 25 to 3. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 2. D 47 7. This is a result of the light being attenuated and scattered by the plastics of the well-bottom which can. Spectramax Multi-Mode Microplate Reader Performance Specifications. Fluorescence intensity values increased over time, but similar EC 50 values of 263 nM and 295 nM were obtained for assay incubation times ranging from one. for the plate. Our absorbance plate readers feature our PathCheck Sensor technology and. assays, we can offer a microplate reader solution to help meet the. A 96-well plate scheme was selected for the measurement mode and the wells selected for targeting the respective colonies arrayed. A . 2 only) SpectraMax M-series Microplate readers. Tecan Infinite F200 Fluorescence Microplate Reader. . I am using the filter pair of 480/535 and trying to measure fluorescence. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633 (18) SARS-CoV-2 antibody signatures robustly predict diverse antiviral functions relevant for convalescent plasma therapy medRxiv September 16, 2022 Harini Natarajan. 25 to 3. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for. Also refer to this Multimode Reader Comparison Chart. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. A solution for low volume applications. Molecular devices SpectraMax M3 Pdf User Manuals. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. 304 signal level saturation. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. Fixes: General:SpectraMax M2 and M2e Multi-Mode. (30. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. 00 USD. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. 119. 25 to 3. VANTAstar™ Flexible Multi-mode Microplate Reader. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Avoid losing high signal. Perkin Elmer 1420-2560 Victor X Series Plate Reader. 67. SoftMax Pro. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. read matrix, spectral scanning and detection of plate formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. When connecting for the first time to either the SpectraMax L reader or StakMax system. free tracer decreases, resulting in an overall increase in RFU values. 0) OD (500 to 1100) RFU (5. The SpectraTest absorbance test plate (Abs-1) can be used by VersaMax, SpectraMax, & M2 readers. (b) Fluorescence responses of probe HADP and control compounds. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. 10. Fig 2: Enzymatic response of synthetic compounds. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. It allows you to explore cellular pathways and protein activation and expression in one system. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance. Single-Label Plate Reader: SpectraMax Plus 384 (Molecular Devices). 8 is required to operate the new SpectraMax® M5 reader and its five read types: Fluorescence Intensity, Absorbance, Luminescence, Time-Resolved Fluorescence (TRF) and Fluorescence Polarization. Detection modalities include absorbance (UV-Vis Abs) and. Calcein AM is a widely used live-cell marker. Fluorescence measurements were acquired with a Molecular Devices SpectraMax M2 Microplate Reader using 96 well plates, with the samples being excited at 468 nm and emission being measured at 572 nm. The instrument is located in room 2131 at 300 George Street. SoftMax Pro Software has been integrated by many leading robotics and LIMS partners, enabling both data analysis and. 306 plate air cal fail,low light. Download PDF. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The SoftMax Pro Software validation package and IQ/OQ validation protocols include tools for FDA 21 CFR Part 11 compliance. Download Application Note Plate reader settings for NanoOrange assay. Three data types for FP read types are also being introduced with v4. Multimode Microplate Readers. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Analyze samples using fluorometry, photometry, luminometry, TRF or FP with these accurate, versatile microplate readers. SpectraMax ABS Plus SpectraTest ABS2 v1. However, proNGF-induced receptor complex formation has been difficult to directly assess other than by western blotting. すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. Optimized for fluorescence microplate readers. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. 3 foot) See. Analyze samples using fluorometry, photometry, luminometry, TRF or FP with these. Microplate Readers Include the M3, M4, M5, and M5. The first step consisted of taking an initial fluorescence measurement using Molecular Devices SpectraMax® M2 fluorescence plate reader of a 96-well plate containing PET-RAFT polymerization reaction mixtures in individual wells. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 00. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading. LVF Monochromator™ : Excitation and Emission. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. SpectraMax Glo Steady-Luc Reporter Assay Kit. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. . 5,112,134 5,766,875. A microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. Add to Cart. 2. Shop all used microplate readers and parts from a variety of manufacturers. With optimized reagents, option to operate the readers. The microplate reader will read the plate and the relative fluorescence units will be displayed in the Plate section. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR. At different time points, the plate was removed from the incubator and loaded to the plate reader for fluorescence reading (excitation 450 nm,. orporation. Compact and tunable absorbance microplate readers DATA SHEET. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF. Standard. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. SpectraMax M2 and SpectraMax M2e from Molecular Devices are multi- detection microplate readers with dual-monochromators, dual-mode cuvette ports and top- and bottom-reading capability (top-reading only on SpectraMax M2). SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. e. It can measure fluorecence from the top or bottom of a plate with a range of 250 - 850 nm. Fill a clean plate with water. When a feature or capability applies to only certain readers, this exception is noted. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. We would like to show you a description here but the site won’t allow us. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. Fluorescence Microplate Reader Comparison Chart. Specialized microplate readers for luminescence detection are available for use in applications such as luciferase reporter assays, bioluminescence resonance energy transfer (BRET), and other luminescent assays. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). 0 out of 5 $ 3,895. In the lower half of the window, left side, there is a box labeled reader. 2. , a positive control). 0 out of 5 $ 9,995 $ 9,495. SpectraMax Microplate Readers. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. 1 SoftMax Pro v4. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. We use Spectramax M5 microplate reader in our lab. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. fluorescence). The drawing shows a standard microplate of 15 mm height covered with a lid. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. 0077 OD 0. Fully tested with validations and warranty. Self Service Available. 5008531B 3 Contents Chapter1:SpectraTestMulti-ModeValidationPlateOverview 4 Multi-ModeProtocols 6 Chapter2:GettingStarted 8 CertificateofCalibration 8SpectraMax M2. Qualify the fluorescence performance of the SpectraMax . The chamber contains. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. This reader's dual monochromators for. Three kits covering a wide range of dsDNA concentrations. Multimode Readers Comparison Chart. Cells in each image are identified by SoftMax Pro Software and cell-by-cellThe Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. 0. . Summary of Contents for Molecular Devices SpectraMax M2. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Traditional microplate readers perform mostly biochemical assays—measuring how much light passes through or is emitted by a sample. the reader’s stated range. Warranty: 60. For example, microplates containing different samples can be read using the Tecan Infinite® M1000 Pro Plate Reader. Select the Driver tab and click Update Driver. ~ Bio-Tek ELx808 Microplate Reader $2,795. type: Clear: Add to Cart. CO 2 & O 2 control. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader , SpectraMax M5. 1. • Flexible design for future expansion • User upgradable in < 2 minutes • High speed detection for up to 1536-well microplates • Excellent. FI assay. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. 00 USD. The IC50s by cell rounding and LysoTracker assays were 0. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. 87. Reads various kinds of 96-wells plates, and is equipped with shaking function. WinKQCL ® Endotoxin Detection and Analysis Software Version 6 Features. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. free tracer decreases, resulting in an overall increase in RFU values. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Type Clear: Add to Cart. on SpectraMax M2 fluorescence plate reader (Molecular Devices, CA, USA): excitation wavelength 320nm, emission wavelength 470nm. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). 7°C and the bottom to 36.