The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. , contact Molecular. Multimode Reader Comparison Chart. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). BioTek Synergy 2 SL Luminescence Microplate Reader with PC & Gen5 Software. The SpectraMax M3 microplate reader (Molecular Devices, USA) was used to monitor the colour development over 5-min intervals for 30 min at 370 nm. 2. Also refer to this Multimode Reader Comparison Chart. Both readers feature an 8-channel read head for fast reads and. 1 release preventing the protocols from being used for validation. Trends within and amongst the times series, reaction half-lives, and maximum chemiluminescent intensities are discussed. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Finally, SpectraMax M3 microplate reader was used to detect 412 nm absorption values, which were positively correlated with GSH levels. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. 3: The cuvette chamber. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. 1step assay with SpectraMax microplate readers. After that, 100 µL of TMB solution was added as the substrate and kept for 15 min at room temperature. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. 0200-0729. Spectranet customer portalPage 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. On the front panel of the instrument, press the load/eject button to move the microplate drawer outside of the instrument. Add to Cart. The test was repeated three times. Detection modalities are shown in Table 1-1. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. FlexStation™ I. We would like to show you a description here but the site won’t allow us. A microplate reader as• FlexStation® 3 Multi-Mode Microplate Reader• SpectraMax® i3/i3x Multi-Mode Microplate Reader • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers• SpectraMax® Paradigm® Multi-Mode Microplate Reader 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. The detection of intracellular ROS and mitochondrial membrane potential SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 0200-6117. For all. For example, microplates containing different samp les can be read using the same or We would like to show you a description here but the site won’t allow us. The M3/M4/M5/M5e/Flex3 and the L/LMax II/LMax II 384 groups of readers each require different Dead Time Correction values, and therefore each has their own Certification. The innovative and flexible design features. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 2. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. SpectraMax® ABS. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Instrument settings used with the SpectraMax M5 Microplate Reader. Bottom-reading is usually recommended for cell-based assay with adherent cells. Results. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive dataPlates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Detection modalities are shown in Table 1-1. SpectraMax® M5e. Molecular Devices LLC pro 6 3 software controlled spectramax m3 multimode microplate reader Pro 6 3 Software Controlled Spectramax M3 Multimode Microplate Reader, supplied by Molecular Devices LLC, used in various techniques. Choose from a three- (M3), four- (M4), orThe “M3 M4 M5 M5e ABS1” and “M3 M4 M5 M5e FL1” validation protocols are not included in v5. Speed Read Mode. 315 can't find zero order. HistologyWe have been using a SpectraMax M3 for several years and have found it excellent, but more recently have been looking to use assays which require Time Resolved Fluorescence (TRF) operation (really. Use the 3. A. Data were analyzed using Parallel Line Analysis 2. SpectraMax ® M2 Multi-Mode Microplate Reader. 3 software (Molecular Devices, San Jose, CA, USA). 3) This. Dead Time Correction accounts for the finite amount of time it takes the instrument to detect and then record the absorbance and counting of a photon by the. Dual monochromators increase performance and assay flexibility. The assay was repeated at varying final concentrations (5, 15, 25, 50, 100 nM) of PES. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Robust, high-value microplate. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Microplate Readers Tecan Infinite M200 Plate Reader. . The ALP activity level was determined, relative to that of the control group, as. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. 4. Description 1. SpectraTest ABS1 Absorbance Validation Plate. In this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Expand your lab’s capabilities to include flash applications such as dual luciferase and. • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderWe would like to show you a description here but the site won’t allow us. SpectraMax® ABS Plus. SpectraMax® Aquamax. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellLuminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. 2. For more detailed information and technical support of Life Technologies assays including specific conditions for assay windows between 2-3 fold, please call 1-800-955-6288 and enter extension 40266 or email. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. , Sunnyvale, CA). 0 mm hex key to tighten screws #2 and #3 until the lock is attached to the plate drawer. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. The bacterial colony was mixed with. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Lift the right side of the microplate reader and place the feet into the two mounting cups on the baseplate. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Select wells and then add or select a group or blank and assignA microplate reader that can detect two or more applications is considered a multi-mode plate reader. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. Published Dec 16, 2020 | Updated Dec 16, 2020. The drawing shows a standard microplate of 15 mm height covered with a lid. Guanidine hydrochloride at a final concentration of 5 M was used to denature a 10 mg/mL solution of lysozyme. SpectraMax i3x Multi-Mode Detection Platform. The chaperone function of PfHsp70-1 under various experimental conditions. Microplate Reader Shelf. Microtitre plates were incubated for 2 h at 37°C in the dark. SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3Chapter 1: Introduction The SpectraMax® Mini Multi-Mode Microplate Reader is a multi-detection instrument with a 6- well to 384-well plate read capability. 312 gain calibration failed. This process should take about 3 - 5 minutes. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Page 1 SpectraMax ® GEMINI EM Dual-Scanning Microplate Spectrofluorometer Operator’s Manual 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0088 Rev. A purple product is formed when pLDH is present , and this product is quantified in a Spectramax M3 microplate reader at the absorbance of 620 nm. SpectraMax Paradigm Multi-Mode Safety Guide 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. SoftMax Pro. 2. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). ” Download for Windows of SoftMax® Touch Software. Cables. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Mitosox works fine in 96 wells. The reaction was terminated with 100 µL of dinitrosalicylic acid and placed in a boiling water bath for 5 min. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). Also, when using a SpectraMax M4 Reader,. The entire SpectraMax M range includes a cuvette port for Absorbance, Fluorescence, and from SpectraMax M3 also for Luminescence. Qualify the fluorescence performance of the SpectraMax . iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. We would like to show you a description here but the site won’t allow us. Category 2 and 3 microplate readers use gradient heating as well. Wavelength precision. . Moving a SpectraMax Multi-Mode Microplate Reader. Molecular Devices Spectramax Plate Reader. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. 313 reference gain check fail. We measured the fluorescence intensity for 240 min at room temperature using a spectrophotometer (SpectraMax M3, Molecular devices) with excitation and emission wavelengths of 485 and 525 nm, respectively. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. The actual pathlength, d, of a solvent is found from the following equation: d cm. 2. Spectramax PMT voltage? I used spectramax M3 plate reader to measure the fluorescence change through time course. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. com. Fluorescent Polarization: Solid black plates, as above. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Using a SpectraMax M3 spectrophotometer from Molecular Devices, we measured the color change at 450 nm. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Call 516-939-0888 to check current. ISM Equipment Classification (Group 1, Class A) This equipment is. For crystal violet. Note: you might need to slide the microplate reader on the foam pad to make room for the cytometer. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The results were normalized as percentage based. . e. Absorbance was measured at 490 nm using a microtiter plate reader (Molecular Devices Spectramax M3, Sunnyvale CA, USA). The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. Learn how to set up protocols, customize reports, export results, and more with this PDF document. All associated StakMax® stackers. 2. Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. The chamber. Wavelength Range. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. 4. Luciferase Detection Instrumentation Guide. All procedures completely complied with the manufacturer’s instructions. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. spr SpectraMax iD3 SpectraTest ABS2 v1. spr SpectraMax Plus 384. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. 01) + 0. Other technologies such as imaging. Place the plate drawer transport lock on the end of the plate drawer. SpectraMax Spectrofluorometers. Mitochondrial activity was expressed by the decrease in OD (450–660 nm), discounting the average of the ODs of the controls . Cells were transfected with Sirt1 siRNA or scrambled siRNA. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax ABS Plus SpectraTest ABS2 v1. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. 2. Multi-Mode Microplate Readers. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. ; The top 3 importers of Spectramax are India with 365. A colorimetric assay was performed using a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA) at 450 nm. 1). Aggregation of MDH was monitored as a function of light scattering at 360 nm over 30 min at 51°C in a SpectraMax M3 (Molecular Devices, United States) microplate spectrometer. Shop For Laboratory Equipment featuring a wide variety of new, used and refurbished laboratory equipment. In the M2, M5 and Plus 384, the light. 1. The result was expressed as mg gallic acid. DevicesThe SpectraMax M2 And M2e Systems Provide The Multi Detection Microplate Readers With Dualmonochromators, Dual-mode Cuvette Ports, With Top & Bottom Reading. SpectraMax Gemini EM/XPS. High sample correlation coefficients (R 2) make it easy to. The relative LDH activity was calculated from the slope of the graph of decreasing absorbance. book Page 1 Tuesday,. SpectraMax readers are the only ones on the market with the versatility to evolve and adapt to every need. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. The absorbance reading of the purple product was formed, which was an indication of the pLDH activity and, hence, the number of parasites present was quantified in a Spectramax M3 microplate reader (Abs 620). Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). For more sample throughput, the system canStakMax Handling Stacker Base Unit. The SpectraTest ABS1 Validation Plate package. Fusion of liposomes was followed by content mixing of the RPLs and subsequent increase in fluorescence which was monitored in a SpectraMax M3 fluorescence plate reader (Molecular devices). Examples: “Press carefully to engage,” “ Do not press down. Description 1. The drawing shows a standard microplate of 15 mm height covered with a lid. Lower limits of detection for ATP determined using the ATPlite . When a SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. SpectraMax Gemini, SpectraMax I series, SpectraMax M series, FlexStation 3. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Luminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. Figure 6: Transcreener ADP2 FI assay principle. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide Modes SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. 4 kilograms). Operated from within SoftMax Pro Software, the StakMax Microplate Handler can hold up to 50 plates and facilitates barcode reading. Supplier: Molecular Devices. Choose from a three- (M3), four- (M4), orFluorescence was measured using a SpectraMax M3 plate reader equipped with an excitation at 490 nm and an emission at 535 nm. SpectraMax Paradigm Multi-Mode Detection Platform User Guide Loading and Unloading Microplates To load or unload a microplate: 1. Find many great new & used options and get the best deals for Replacement Bulb for Molecular Devices SpectraMax M3 Flash Lamp at the best online prices at eBay! Free shipping for many products! Skip to main content. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. 313 reference gain check fail. D8000C; R&D Systems, Minneapolis,. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. 9000-0756. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Firefly luciferase, a 61 kD monomeric protein, is especially attractive to many researchers because of its high sensitivity, wide linear detection range and extremely low background due to the absence of endogenous luminescent activity in mammalian cells. For cell viability assay, cells were incubated for 4 h with 1 mg/ml resazurin solution (10 μl), and then the fluorescence intensity was measured using a SpectraMax M3 microplate reader (Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 544 nm and emission wavelength of 595 nm . Additionally, SoftMax® Pro Software automatically identifies the optimal excitation and emission wavelengths via the Spectral Optimization Wizard. The optical density (OD) was read by SpectraMax M3 (Molecular Devices, Sunnyvale, CA). The absorbance was measured at 230. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® iD3 Multi Mode Microplate . {"product":{"total":4,"offset":0,"limit":4,"data":[{"path":"/products/microplate-readers/multi-mode-readers/spectramax-m2-m2e-readers","identifier":"SpectraMax M2/M2e. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. The absorbance was assessed at 405 nm by a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signal A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Use the 2. To assess mast cell degranulation, β-hexosaminidase release was evaluated according to a method published by Dearman et al. Concisely, digested test composite was treated with NaNO 2 (5%) and incubated for 5 min. We would like to show you a description here but the site won’t allow us. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Summary of Contents for Molecular Devices SpectraMax M2. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. Offer Ends In: 5 Days 05: 02: 03. Dual monochromatic, three modes detection, UV Vis Abs, Fluor, and Lum, cuvette. Danaher Inc molecular devices spectramax m3 spectrophotometer Molecular Devices Spectramax M3 Spectrophotometer, supplied by Danaher Inc, used in various techniques. Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds. For example, microplates containing different samp les can be read using the same orCell viability는 세포막 Integrity 또는. Furthermore, to investigate the effects of nucleotides on the interaction, the assay was repeated in the presence of 5 mM. Our Price: $8,635. Turn the instrument around so that the back of the instrument is. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. We would like to show you a description here but the site won’t allow us. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. . From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 50 0112-0115G Item Description Wavelengthrange 200-1000nm Wavelengthselection Monochromator,tunablein1nmincrementsPage 25 SpectraMax iD3 Multi-Mode Microplate Reader Installation Guide To install the transport lock on the plate drawer: 1. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Description 1. SpectraMax® M5. The chamber contains springs that position the cuvette in the proper alignment for a read. book Page 1 Wednesday, July 7, 2010 2:09 PM FlexStation® 3 Benchtop Multi-Mode Microplate Reader User Guide 0112-0127 B June 2013 FlexStation_manual. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. When the user applies a set temperature of 37°C, the top is heated to 37. 9000-0756. The concentration of CTC was expressed as mg catechin equivalent per g of extract. Learn more about its features, specifications, and applications in this user guide. SpectraMax Microplate Readers. DCFH-DA and JC-1 were used to detect intracellular ROS and mitochondrial membrane potential. Figure 1. For wavelengths in the UV range above 220 nm,. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. The relative number of viable cells was quantified by measuring the fluorescence (excitation at 560 nm and emission at 590 nm) using a SpectraMax ® M3 (Molecular Devices, San Jose, CA, USA). Brochure. The DNA extracts were quantified using Quanti-iT™ Picogreen ® dsDNA reagent (Invitrogen, Paisley UK) on a black 96 well plate at an absorbance of 520 nm. In the SoftMax Pro Software, select the Operation tab and click Change Aperture to display a message. Absorbance was read at 570 nm using a SpectraMax M3. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Spectral scans were performed on native and denatured lysozyme samples using the SpectraMax iD3 reader with excitation at 270 nm and emission ranging from 300 nm to 450 nm. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. o For example: Ex: 485/Em 525/Cutoff 515 4. 4. the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. Collection in December 2013 and storage until extraction of Malaysian the A. 304 signal level saturation. We would like to show you a description here but the site won’t allow us. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. The spectrophotometer (SpectraMax, M3, Molecular Devices, Washington DC, USA) was used to confirm the encapsulation of gallic acid inside the PLGA nanoparticles. Description 1. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. D 1 1. Filterless readers provide consistent results with steady temperature regulation. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. Moffitt Cancer Center. 1 & 7. 100 µL of MycoAlert Substrate (reconstituted in MycoAlert Buffer) was immediately added to each well and the plate incubated at room temperature for 10 minutes. 1. Using. How do I do this? Note: Check the Applies to section at the bottom of the page to make this is the. 303 unable to cal dark current. The SpectraMax M2 and M2e are versatile and reliable. Data were expressed as mg/g gallic acid equivalents (GAE) of dry crude extract. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part. -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for If application. To avoid injury, it is recommended that two. The optical densities (OD) were determined using the SpectraMax ® Plus 384 Microplate Reader (SpectraMax M3; Molecular Devices, SanJosé, CA, USA) at 450 nm and 660 nm [31,34]. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Superior flexibilityThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The guide also covers the software features, the specifications, and the accessories of the systems. The reading was taken with a spectrophotometer (SpectraMax M3) at 640 nm. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Microplate Reader Shelf. With optimized reagents, option to operate the. com. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. . It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. I am looking to meet/connect with people from all walks of life. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. gingivalis -treated BMDMs (MOI = 50), with or without pretreatment of OTTSP167 or Compound 50 for 2 h. 3) At the bottom of the strip, click on "Show. Cells were treated with potassium bromate (30 mM) to provide a pro-oxidant source required to lower the glutathione ratio, which was used as a. no. 0. 10. As for each measurement, I. book Page 1 Wednesday, July 7, 2010 2:09 PM When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. 2 only) SpectraMax M-series Microplate readers. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Plate Formats: 6, 12, 24, 48, 96, 384 wells. Learn more about its features, specifications, and applications in this user. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Bioz Stars score: 86/100, based on 1 PubMed citations. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. 2. 306 plate air cal fail,low light. 4. SpectraMax® M4. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Male 8 pin mini Din to Female 9D. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. To determine growth rate, the log phase of growth. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 303 unable to cal dark current. Photometric accuracy. The information below applies to a standard 96 well plate, set to a linear shake mode. FlexStation™ III. StakMax Microplate Handling Stacker. Multimode Readers Comparison Chart. The SpectraMax ABS Plus reader utilizes Molecular . Choose from a three- (M3), four- (M4), orThe SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Cells cultured in 96 well plate are washed with PBS or HBSS (phenol red free), incubated with ~5-10 uM. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The ROS level was detected by a reactive oxygen species assay kit (Beyotime, China). The detection of intracellular ROS and mitochondrial membrane potential. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 190-1000 nm, tunable in 1 nm increments. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today.