Designed to provide the simplicity, flexibility and power required for advanced data analysis. Incubation. SpectraMax M Series multi-mode microplates are configurable readers with triple. Step 2. The CellTiter-Glo assay from Promega uses luciferase. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. 3 mg. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Note: In this user guide, SpectraMax ® refers to several SpectraMax . The PBS solution (10. The readings with excitation at 360 nm and emission at 450 nm represented the amount of. The integration time suggested is 5-10 sec. When a feature or capability applies to only certain readers, this exception is noted. For the Live/Dead assay, they were treated. 7 cells were pretreated with SG (0, 25, 50, and 100 μg/mL). SpectraMax M5e Multi-Mode Microplate Reader. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the. Read one sample or up to 384 inThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). view more . • Tel. Introduction. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. Bioz Stars score: 86/100, based on 1 PubMed citations. Wide range of applications. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown menu. When a SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Wide range of applications. This application note describes how to. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. 5 Compatible microplate readers FP Plate Reader SpectraMax® M5/M5e Multi-Mode Microplate ReaderSpectraMax M5 Multi-Mode Microplate Reader; Method. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The cuvette must be in place when you read the plate. The spectrophotometric. The SpectraMax® ABS Plus Microplate Reader can run . These robust readers have been placed in labs from Antarctica to. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. . Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. reader in the visible range between 405–750 nm with NIST and NMI. After two more rinses with Hank’s solution, fluorescence intensity of the red/green ratio was determined on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at an excitation of 490 nm and emission of 530 nm (green fluorescent monomers) and 590 nm (red fluorescent aggregates) respectively. SpectraMax® M5 Multi-Mode Microplate Reader (cat. Documentation Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Parallel negative control assays had primary, secondary, or both antibodies omitted. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. Molecular Devices. The SpectraMax M5e Multi. AquaMax® 2000. Lift up the lid over the chamber to insert or remove a cuvette. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. SpectraMax® M5e. If you do not have an iLab account you must first make one: would like to show you a description here but the site won’t allow us. 5 Dual-luciferase reporter assay. Call 516-939-0888 to check. Choose cables that meet the requirement described in this appendix. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. SpectraMax M5 reader settings are shown. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. The PBS solution (10. Microplate Readers include the M3, M4, M5, and M5e models. 96-1536. While they play an important role in generating data from a wide range of. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. Five. 0 out of 5 $ 17,999 $ 16,995. SpectraMax® M5e. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Ltd. Bookings are free, but you will need to use iLab to book. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. SpectraMax M5e Multi-Mode Microplate Reader. Microplate Reader, MultimodeProducts : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. These lab workhorses are used in drug discovery, research, bioassay validation, and. The dual monochromator optics allow the widest. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideSpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. STAKMAX. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Molecular Devices. Add to Cart. Offer Ends In: 44 Days 10: 22: 56. 1 & 7. We would like to show you a description here but the site won’t allow us. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. Table of Contents. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. KEY FEATURES • Five. The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. Recommended Optics Wavelength (nm) Wavelength. Fluorescence was measured (Ex 530/Em 590) in a SpectraMax M5 microplate reader. Category 2 and 3 microplate readers use gradient heating as well. com 13. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Path Check Pathlength Measurement SpectraMax Spectrofluorometers. Table of Contents. The SoftMax® Touch. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The Spectr. The assay applies fluorescence polarization to quantify Fc-containing IgG. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. B: Selection of Excitation Wavelength. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModePlate chamber design of the SpectraMax category 1 microplate readers. Category 2 and 3 microplate readers use gradient heating as well. Step 1. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. 00 USD. The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. Microbiota DNA was. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance,. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Then, after H 2 O 2 was added, the fluorescence was measured for each time period at 488 nm (excitation) and 525 nm (emission) wavelengths on a SpectraMax M5 microplate reader. Figure 4: Transcreener ADP. free tracer decreases, resulting in an. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. 4. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 0 . Figure 4: Transcreener ADP. M3-M5e_manual. AquaMax® 4000. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. com A. 67. For more sample throughput, the system can Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. 2. We would like to show you a description here but the site won’t allow us. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. E Models Only) 69. Molecular Devices Corporation. Catalog Number: (89212-396) Supplier: Molecular Devices. The following document is intended to demonstrate setup of this instrument. These robust readers have been placed in labs from Antarctica to the. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Read the Cuvette 1. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. 4 0. A microplate reader asThe SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. free tracer decreases, resulting in. This. SpectraMax® Aquamax. One vial of Thaw-and-Use TIGIT Effector Cells was thawed and resuspended in cell recovery. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. SpectraMax® ABS Plus. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Nontreated cells in DMEM medium were used as a control. 7 1. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. M3-M5e_manual. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Microplate Readers Biotek Epoch Microplate. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 00. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. The guide also covers the software features, the specifications, and the accessories of the systems. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. 63 1. ZERO BIAS - scores, article reviews, protocol conditions and moreThe absorbance was measured at 450–650 nm using a SpectraMAX M5 microplate reader (Molecular Devices). Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. TNF standard curve. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Recertification is recommended every two years. With optimized reagents, option to operate the. Add to Cart. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. 2. Fluorescence intensity was detected by. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 6-384. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. , Sunny-vale, CA). : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. 00 USD. FlexStation™ III. 40266 Email: drugdiscoverytech@lifetech. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The chamber contains springs that position the cuvette in the proper alignment for a read. Bottom-reading will also give you higher RFU's (and the appearance of high sensitivity) because the polystyrene will fluoresce a bit. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Introduction. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. 2. 05 3. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Luminescence microplate readers or luminometers are used to detect luciferase-based reporter gene assays, chemiluminescent ELISAs, BRET, and many other assays. This reader's dual monochromators for. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Examples: “Press carefully to engage,” “ Do not. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Go to Product Details. Most papers they cultured bacteria to OD600>0. Self Service Available. Microplate Readers are modular and . Italic and boldface type are used for emphasis. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Then, the fluorescence change rates (F 0 − F)/F 0 were calculated, and the selectivity histogram. Molecular Devices Spectramax M5 Microplate Reader. The dual monochromator optics allow the widest range of applications to be. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. All reagents were brought to room temperature before use. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Western blot analyses. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. Microplate Readers include the M3, M4,. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. ) injection of 3 mLThe fluorescence intensity was detected by using a SpectraMax M5 microplate reader from Molecular Devices Co. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. No accessories are needed and SoftMax® Pro Software. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Add to Cart. The. Microplate Readers Biotek Synergy H1 Hybrid Microplate. No filter change with dual monochromator optics. Read More. 49 2. The dual monochromator optics allow the. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. 6: bioNova científica, s. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. SpectraMax Lmicroplate luminometer. 3. Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. The inset, using the same data set, highlights the sensitivity of the assay at low levels of H 2O 2. Figure 1. Linear, Orbital Slow, and Orbital Fast. 7 cells and HEK293T cells were seeded at the density of 2 × 105 cells per well in a 24-well plate overnight. Figure 1. flexibility of monochromator-based systems. The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 7 cells were co-transfected with 0. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader;. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. PMT sensitivity was set to AutoRange with a target calibration wavelength of 470 nm, and the plate was dark adapted for 1 minute prior to reading. 1. SpectraMax M3, M4, M5 and M5e Multi-Mode Microplate Readers User Guide Fluorescence Microplate Reader Comparison Chart. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. 96-1536. With optimized reagents, option to operate the readers. Compatible automation solutions for SpectraMax Readers. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. View the Molecular Devices website. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. analysis, controls the SpectraMax Multi-Mode Microplate Readers. Here the delta mP was 336, and Z’ factor was 0. com. Also refer to this Multimode Reader Comparison Chart . These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. PURPOSE. The cells were incubated with compounds at the indicated doses for 1 h. Page 28 Chapter 5: Read Modes and Read Types 1. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. SpectraMax® iD5. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Both readers feature an 8-channel read head for fast reads and. 14 Average %CV 0. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. Microplate reader settings. S. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. When the user applies a set temperature of 37°C, the top is heated to 37. TR-FRET assay principle. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode. SpectraMax® M4. Molecular Devices. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax M5 microplate reader (Molecular Devices, California, USA) with excitation at 450 nm and emission at 570 nm. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. This is equivalent to having 511 built-in filters,The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. 67. A: Microplate Preparation. 00. Five preferred detection modes. 00 USD. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Selection of Water Constant in PathCheck settings. Temperature variation ± 0. SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E. AquaMax® 2000. 73. Bookings are free, but you will need to use iLab to book. ® reader to the computer. book Page 1 Wednesday, July 7, 2010 2:09 PM The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. 04 to 3. 4 Max StDev 1. Before you Begin Materials Required but Not Provided • Fresh, anhydrous. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Molecular Devices SpectraMax M5 ; Molecular Devices SpectraMax M2. Lower limits of detection for ATP determined using the ATPlite . The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The assay window spanned four orders of magnitude. Microplate Capacity. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the signal is constant. Add to Cart. RAW 264. 0 OD for earlier models. SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです！ NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. We would like to show you a description here but the site won’t allow us. 5. Trilevel commercial control reagents (StanbioThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. p. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. e. Wide range of applications The VersaMax reader covers. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. Cuvette port functionality can also be controlled using SoftMax Pro Software. The calculated LOQ values were 0. 7°C and the bottom to 36. Plates were then shaken at room temperature for 10 min and the absorbance at 570/630 nm was measured by using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). 1. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability.