For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. edu) to be added as a trained user in iLab. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. 305 reference level saturation. com A. SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5, Analyst HT or Analyst GT. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Refer to “Installing Detection Cartridges” in. View the Molecular Devices website. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. . . For SpectraMax L or LMax II, LMax II. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Spectramax M5 plate reader (96/384 format) Multimode photometrics for endpoint or kinetic measurements. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. Choose from a three- (M3), four- (M4), orThe SpectraMax M5, M5e, and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. Highly selective for dsDNA over ssDNA or RNA. The KeySpan USA-19HS is often required with new Windows 7. The plate reader can measure multiple readouts, including UV and visible absorbance. The M5 has two PMTs. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Read More. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. ScanLater Western Blot Detection Cartridge. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. With optimized reagents, option to operate the. Optical absorbance was measured at 570 nm on a SpectraMax M5 microplate reader. Step 4. . 1321N1, a human astrocytoma cell line, expresses. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Catalog Number: M5. Description 1. 00 USD. 5. Products : SoftMax Pro GxP Software, SoftMax Pro Software, SpectraMax M5/M5e reader. 4. Add to Cart. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Small, unbound molecules rotate rapidly in solution (top), while large,. Table of Contents. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. The. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. HEALTH AND SAFETY CONSIDERAIONSThe SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. 6: bioNova científica, s. 72 MB. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity, permittingwidespreaduseincludingELISAs,proteinquantitation,endotoxinassays,and cytotoxicityassays. Purchase Overlord with the required number of. We would like to show you a description here but the site won’t allow us. Cytokines in medium released. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The substrate solution (100 μL/well) was subsequently employed for 5-min reaction and the absorbance at 370 nm was monitored on a SpectraMax M5 plate reader (Molecular Devices). 3. 72 MB. . Wavelength optimization. Molecular Devices SpectraMax PLUS 384 Spectrophotometer System-New ABs1 Performance Testing August 2021. 313 reference gain check fail. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Molecular Devices. No filter change with dual monochromator optics. Bottom-reading is usually recommended for cell-based assay with adherent cells. . This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. HEALTH AND SAFETY CONSIDERAIONSThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M5 Multi-Mode Microplate Reader. +1 800-635-5577. We would like to show you a description here but the site won’t allow us. 5. . 304 signal level saturation. Also refer to this Multimode Reader Comparison Chart. . ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e• SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an. clicked with the SpectraMax L Microplate Reader. Multimode Reader Comparison Chart. In this. The dual monochromator optics allow. The corrected absorbance is within 5% of the. Fluorescence spectra and UV–vis spectra were recorded on a SpectraMax ® M5 multi-mode microplate reader (Molecular Devices). Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. Figure 1. SpectraMax M5 Multi-Mode Microplate Reader. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. 00 USD. Molecular Device SpectraMax M5 Plate Reader - Certified with Warranty. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. . The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. 0200-7027. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Step 1: Open the SoftMax Pro IMAP FP protocol. Step 1. . The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET. Settings not required by a reader are indicated by ‘---’. Note: In this user guide, SpectraMax ® refers to several SpectraMax . For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The SpectraMax M5/M5e & FlexStation 3 instruments use SoftMaxPro® Data Acquisition & Analysis Software. . Published Dec 16, 2020 | Updated Dec 16, 2020. The M5 can read volumes down to 2uL with the uMax Low Volume. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. 3. I have been using the SpectraMax M5 since 2011. TR-FRET assay principle. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. These all-inclusive assay kits are ready to use in assay validation or screening. ORDER. D 1. Table of Contents. ; The top 3 importers of Spectramax are India with 365. The following document is intended to demonstrate setup of this. Three kits covering a wide range of dsDNA concentrations. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. 306 plate air cal fail,low light. Table of Contents. For wavelengths in the UV range above 220 nm,. l. 多功能酶标仪 FlexStation3 快速动力学操. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a. 40266 Email: [email protected]® M5多功能读板机为使用者提 供了除化学发光以外的其他检测方法，例 如：光吸收，荧光强度，荧光偏振和时间 分辨荧光。 SpectraMax® L和SpectraMax® M5酶标 仪均用SoftMax® Pro软件进行操控和设 置，该软件含有CellTiter-Glo检测模板和0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideExplore how the SpectraMax i3 Multi-Mode Platform provides an unlimited breadth of application possibilities, and how it can evolve with your future needs. In either case. 9000-0756. Five preferred detection modes. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Description. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 7 nM EGF, the EC 80 concentration determined from. Open the Softmax Pro software and select the FP option. Typical LLDs were approximately 1–2 fg/well in the Analyst GT and 2–3 fg/well in the SpectraMax M5. The SpectraMax M5 is a microplate reader that can be used for various applications,. 5. 1. Step 4: Incubate at room temperature for 1 hour, protected from light. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 26 5039228D Note. 3. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. In addition, the SpectraMaxMolecular Devices. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. , ~488 nm), has the optical. In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. 0 out of 5 $ 15,999. Maximum signal with lowest background. item 6 replacement bulb for molecular devices spectramax m5 xenon replacement bulb for molecular devices spectramax m5 xenon. Multimode Readers Comparison Chart. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested. e. Features. . Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. 190-1000 nm, tunable in 1 nm increments. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. The following document is intended to demonstrate setup of this instrument. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. When the user applies a set temperature of 37°C, the top is heated to 37. Cet appareil ISM est confomre à la norme NMB-001 du Canada. 304 signal level saturation. For example, microplates containing different samples can be read using the The interval between reads was set at 15 s or 60 s, respectively. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. When the correct COM port is selected, the. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic Gq-coupled receptor. 4. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. 6. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 190-1000 nm, tunable in 1 nm increments. FlexStation® 3 Multi-Mode Microplate Reader. . The SpectraMax ABS Plus reader utilizes Molecular . UV/Vis Photometric Performance:. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 95. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. The assay window spanned four orders of magnitude. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. 7. Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit The Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Mass spectra were recorded using an HPLC-System Series 1100 coupled with ESI-single. . Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. you can use a resource. New drivers take 6 days to write, existing drivers take 2 days to convert to Windows 7/10 compatibility. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. used. Softmax Pro GxP Software 6. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. SpectraMax M5 microplate reader. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. Molecular Devices also offers a mass spectrometry-based solution for food safety testingMOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. Cell-based experiments In the first experiment, we prepared a dilution series of CHO-K1 cells transiently transfected withThe SpectraMax 190 reader is the tool for DNA analysis. 7°C (gradient heating design). Open the Softmax Pro software and select the FP option. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax ® Pro Software. $4,499. . The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Absorbance (UV – VIS) Fluorescence; Glow luminescence; Time-resolved fluorescence; Fluorescent polarization; ABI Viia7 qPCR instrument (96 format) – SYBR/FAM/VIC/ROX calibrated. The SpectraMax M5 Multi-Mode. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. Description 1. Description 1. 1. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Open the SoftwarePro® Data Acquisition & Analysis software,. We would like to show you a description here but the site won’t allow us. The test solution was by diluting FCV to PBS/ Glycerol solutions (5 μM, 1. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. . Exhibit with us {{>currencyLabel}} Back. KeySpan adapter for SoftMax Pro and SpectraMax readers. +44-118-944-8000. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. Other technologies such as imaging. Add to Cart. The chamber contains springs that position the cuvette in the proper alignment for a read. OptimizingAbsorbanceReadMode The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 4. 多功能酶标仪SpectraMax M5e. . The assay applies fluorescence polarization to quantify Fc-containing IgG. . Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Table of Contents. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. D 1 1. SpectraMax®L Luminometer User Guide 0112-0174 B 11 The SpectraMax L Luminescence Microplate Reader can accommodate SBS-standard 96 and 384-well microplates with dimensions of 128. Bookings are free, but you will need to use iLab to book. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Set the excitation at 531 nm and emission at 595 nm. Spectral Range. Description 1. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. LUMINESCENCE The SpectraMax M2 and SpectraMax M2e are microplate spectrofluorometers with photomultiplier tube detection. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15. SpectraMax M5, Analyst HT or Analyst GT. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax® M5 Multi-Mode Microplate Reader is capable of measuring UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, and TRF. spr SpectraMax iD5 SpectraTest ABS2 v1. Supplier: Molecular Devices. Select the fluorescence option and read the total fluorescence. 3. These robust readers have been placed in labs from Antarctica to the. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Find regional support. HEALTH AND SAFETY CONSIDERAIONS The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Detection of pro-inflammatory factors. Last lab had ours for years and never had a problem with it. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single. . Pick the best speed, sensitivity, and price for your needs. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. ® reader to the computer. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The following document is intended to demonstrate setup of this instrument. Overall, SpectraMax M5 is a powerful lab tool that combines advanced detection capabilities, user-friendly software, and comprehensive accessory options. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader Why does "Path?" appears in all wells when PathCheck is selected? Cause: Water Constants. Control Panel The control panel houses the instrument status LEDs and the microplate. It may be installed in the top or bottom read detection cartridge transport. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Qualify the fluorescence performance of the SpectraMax . Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. . Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Please Inquire. SpectraMax M5e Multi-Mode Microplate Reader. DNA provides the same sensitivity as a spectrophotometer. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. We would like to show you a description here but the site won’t allow us. 1. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideIn this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. SpectraMax Spectrofluorometers. The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Turn on the microplate reader. D 1 1. . Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. If you do not have an iLab account you must first make one: Calcium Assay Kits are the platform of choice for measuring changes of intracellular calcium for drug discovery and basic research. Optimal instrument settings are the same for all three readers. The dynamic range of the assay was approximately 5 orders of magnitude. 2 mm long, 86. When the user applies a set temperature of 37°C, the top is heated to 37. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Reduces well-to-well variability, improving assay quality (Z' factor) and reliability (CV %) of high-throughput. 2. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Some luminescence applications, such as gene reporter assays, may require a luminometer with photon counting detection for greater sensitivity, such as the SpectraMax M5, Analyst GT or Analyst HT, or LMax II. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The SpectraMax M5 Microplate Reader uses SoftMax® Pro. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96-well black plates. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 1step assay with SpectraMax microplate readers. 2. SOFTmax PRO UserÕs Manual iii Contents Figures . Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. ATP standard curves read on SpectraMax microplate readers. Access this by going to Help >. The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e).