Absorbance reading with SpectraMax® microplate readers and SoftMax® Pro Software enables optical density measurement of samples in microplate or cuvette format. The eight-channel system- consisting of 8 sample beams and 8 reference beams delivers both superior. The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. microplates or in a cuvette at a. SpectraDrop High-Throughput Screening (HTS) Kit. . Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Multimode Microplate Reader. The SpectraDrop Microplate incorporates a specially designed adapter and a slide pair whose optical clarity allows measurements in absorbance and. Molecular Devices Spectramax iD3 Microplate Reader. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. When read on microplate readers, the SpectraMax Quant dsDNA Assay Kits enable optimal results and increased assay throughput. 7°C and the bottom to 36. With optimized reagents, option to operate the. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Offer Ends In: 18 Days 19: 38: 21. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. The SpectraMax 190 reader can be integrated with Molecular . Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. 3) This warranty. SoftMax® Pro 7. The instrument uses a paired monochromator and xenon flash. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Other technologies such as imaging. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Summary of Contents for Molecular Devices SpectraMax M2. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. 1. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Download the SpectraMax Mini Reader Plug-in and associated guides. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. Lift up the lid over the chamber to insert or remove a cuvette. 2 software: see SoftMax® Pro 7. Other technologies such as imaging. The guide also covers the software features, the specifications, and the accessories of the systems. western blot membrane detection in a multimode microplate reader platform. When the user applies a set temperature of 37°C, the top is heated to 37. Italic and boldface type are used for emphasis. Category 2 and 3 microplate readers use gradient heating as well. Examples: “Press carefully to engage,” “ Do not. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 1 software Download page. 305 reference level saturation. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. The plate reader optics are tunable for absorbance. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command in SoftMax® Pro. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Avoid losing high signal. Example: [Drawer]. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. The Gemini EM can sample a variety of formats, from 6- to 384-well. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. SpectraMax M5 reader settings are shown. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. For a higher . The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Download PDF versions of the SpectraMax Microplate Reader User Guides. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 7°C (gradient heating design). no. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Microplate reader assays requiring infrared (IR) or red-shifted. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. 303 unable to cal dark current. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. This unlocks the transport slide. Reader modes are customized to your specific applications with options to upgrade easily. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Devices’ StakMax® Microplate Stacker in a matter of minutes and . In addition, the SpectraMax iD5The SpectraMax® L Luminescence Microplate Reader is a high-performance microplate reader that measures luminescence in 96 and 384-well microplates. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Table of Contents. 315 can't find zero order. . Contents of wells were pipetted up and down several times to mix. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalSpectraMax Microplate Readers. Detailed specifications of each model reader are reported in Section 7, “Specifications”. 0 out of 5 $ 5,999. The plate. Win7 Manual Spectramax-L or StakMax Driver Installation after Initial Install. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Fluorescence Microplate Reader Comparison Chart. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. The following document is intended to demonstrate setup of this instrument. All stand-alone instrument functions can be controlled using the software. SpectraMax Spectrofluorometers. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 1000 nm). The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. . Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. More Product Information. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. 0 out of 5 $ 11,999. Find regional support. Positions H21-K22 contained plate blanks of PBS only. SpectraMax Plus: SpectraMax Plus. In addition, SoftMax Pro Softwareluminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. Sale. SpectraMax® Microplate Readers and SoftMax® Pro Software. SpectraMax Microplate Readers. . Cell Cytotoxicity assay procedure. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. 7°C and the bottom to 36. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. 020 fmol 0. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. For more sample throughput, both readersSupplier: Molecular Devices. Due to elevated security of a Windows 7 64-bit operating system, the USB instrument drivers may not be installed automatically. Temperature control. A microplate reader as The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. This pathlength correction is accomplished only with the use of. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. We would like to show you a description here but the site won’t allow us. IMAP FP Settings in SoftMax ProSpectraMax M5 Multi-Mode Microplate Reader. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. View the Molecular Devices website. 1. The SpectraMax microplate readers can be used to accurately quantify ROS levels using a luminescence-based assay. This system includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. Using the optimized wavelength settings, the SPECTRAmax GEMINI XS fluorescence microplate reader can distinguish between live and dead cells using this assay. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. With optimized reagents, option to operate the. Molecular Devices. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. 0 out of 5 $ 5,999. Note that this procedure is only for the SpectraMax L model. Or, with the reader specifications noted from the Instrument Description section of your reader's. We would like to show you a description here but the site won’t allow us. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. All . In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. MPN:SpectraMax Plus. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. It features a modular design that allows users to add or change detection cartridges according to their needs. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Read More. The SpectraMax ABS Plus Microplate Reader is a compact and adaptive microplate reader capable of quantitating nucleic acid and protein samples in various formats such as cuvettes or microplates. 5065390D 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 ComputerIntegration 8 PlateControls 9 CuvetteChamber 10SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. begin reading microplates with seven mouse clicks. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. ELISA Plate Readers / Microplate Readers. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Molecular Interactions. Page 35: Setting Up The Instrument SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Tryptophan, which excites maximally around 270-280 nm and has an emission peak near 350 nm in water, dominates the emission of proteins and is the mostPhysical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. Here, the CVs for each plate were less than 3, and the difference between mean. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Molecular Devices. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. 306 plate air cal fail,low light. Microplate Spectrophotometers. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. Example: [Drawer]. For readers without a cuvette port, simply check the box next to ‘PathCheck’. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. We would like to show you a description here but the site won’t allow us. Wide range of applications. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity. 500 Euro/Dollars for a basic filter-based ELISA microplate reader and could go up to over 150. Analytical Technologies Group specializes in the maintenance and repair of Molecular Devices microplate readers, including the SpectraMax® and VersaMax™ absorbance readers. The screw remains inside the instrument. Instrument settings used with the SpectraMax M5 Microplate Reader. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. Figure 1. Nuclear Receptor. 300 µL of Detergent Compatible Bradford reagent was added to each well. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. . A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. 2 only) SpectraMax M-series Microplate readers. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. With optimized reagents, option to operate the. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. Learn more about its features, specifications, and applications in this user. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. 012 fmol 0. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Figure 1. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Brochure. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today launched its. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. In . Molecular Devices SpectraMax M2E Microplate Reader. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Use the provided 3. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD5 reader. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. A new technical approach to platelet aggregation studies. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. The advanced optical and. 4 μL DNA standards. It can run samples based on pre-defined. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Both readers feature an 8-channel read head for fast reads and a subset feature, the temperature independent PathCheck sensor that. Use the provided 3. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. 315 can't find zero order. Additionally, user-installable options allow the SpectraMax i3 System to. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 5 12. Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution: The SpectraMax® Mini Multi-Mode. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Please refer to the Obsolete Microplate Reader Models for more information. Guides: SpectraMax Mini Installation Guide. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Type: Clear: Add to Cart. The drawing shows a standard microplate of 15 mm height covered with a lid. SpectraMax® Mini Multi-Mode Microplate Reader for Biotech Laboratories Built on the company’s nearly four decades of experience delivering high-performance microplate reader technology to the market, the SpectraMax Mini reader is robust enough to meet a lab’s core microplate reader needs, while its compact size is ideal for integration into a. Request a Quote!Introduction. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. For SpectraMax ABS Plus reader, r 2 = 0. The screw remains inside the instrument. 0 out of 5 $ 2,999-20%. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. Measure more accurately. Fill a clean plate with water. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Other features and benefits include: Eliminate changing out filters. Results are shown in Table 1. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Large, high resolution touchscreen interface allows you to easily set up methods, run experiments or view tutorial videos without the need for a dedicated computer workstation. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). 2. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. Step 2: Launch SoftMax Pro Software. Figure 1. Cell Cytotoxicity assay procedure. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Use similar settings for other Molecular Devices readers with fluorescence detection mode. 313 reference gain check fail. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. . SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Maximum signal with lowest background. with SpectraMax Microplate Readers Introduction Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The SpectraMax® ABS Plus Microplate Reader can run . For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The Spectramax 250s xenon flash lamp and grating monochromator facilitates direct UV measurement of DNA, RNA, and protein in microplates. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader. both cuvette-based and microplate reader applications on the same instrument. HTRF-compatible SpectraMax Multi-Mode Readers. SpectraMax Mini Plug-In for SoftMax Pro 7. Create custom fluorescence assays without having to purchase expensive filter sets. To resume collecting kinetic measurements within the same file, press the Append button. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. The guide also covers the software features, the specifications, and the accessories of the systems. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. Access this by going to Help >. The system uses two scanning monochromators to determine the optimal excitation and emission settings. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 5 87. Next generation SpectraMax® absorbance microplate readers. Sale. See SpectraMax® Microplate Reader User Manual Download Page. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Products. flexibility of monochromator-based systems. The advanced optical and. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Chapter5:ReadModesandReadTypes 0112-0115J 27 Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. We use Spectramax M5 microplate reader in our lab. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. Compare plate readersFive-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. Introduction. Also see:. Absorbance (optical density, OD) was read at 570 nm and 605. 316 grating motor driver faulty. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. SpectraMax® 190 absorbance plate reader enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Lower limits of detection for ATP determined using the ATPliteAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. SpectraMax Quant dsDNA Assay Kits are highly selective for dsDNA. Add to Cart. The SpectraMax® iD3 multi-mode microplate reader measures absorbance, fluorescence and luminescence and is ideal for busy multi-user, multi-application labs. 2. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. . 1. zip. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. Step 2: Launch SoftMax Pro Software. Add to Cart. Temperature control. 0 out of 5 $ 11,999. Detailed specifications of each model reader are reported in Section 7, “Specifications”. edu) to be added as a trained user in iLab. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. This variable pathlength makes it dif Þcult to perform extinction-based assays and confusing to compare results between microplate readers and spectrophotometers. 1. Our specialization servicing. 72 MB.