0A Sunnyvale, CA Made in USAFilter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 40266 Email: drugdiscoverytech@lifetech. Instrument settings can be saved as a protocol file. First, as mentioned before, the walkaway time significantly. tools to expand the boundaries of their research. The Molecular Devices FilterMax F5 (replacing our old Analyst plate reader) is a versatile instrument that offers detection based on fluorescence, fluorescence polarization, luminescence, absorbance, and time resolved FRET. 1. If no sound is heard during initialization, contact technical support. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. FlexStation™ II Systems provide integrated fluid transfer capability from a source plate or reservoir to an assay plate with concurrent kinetic fluorometric analysis of reactions. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. 0A-Sunnyvale, CA-Made in USAMolecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. 9000-0756. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. BMG Labtech - THERMOstar. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 1. Reader Documentation Version No. Plate Formats: 6 to 384-well microplates. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. The plate was then read on Molecular Devices microplate readers with luminescence detection mode (Read A). Cells in each image are identified by SoftMax Pro Software and cell-by-cellOne plate carrier adapter is provided with the instrument. Since cells are nearly confluent, the protocol. Data analysis. Supersedes 1. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. 1 second up to 30 seconds. Set up Identify Acquire Analyze Imaging with the SpectraMax MiniMax Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3 System. Every step is optimized to simplify analysis and reporting to support our microplate readers. 314 low lamp level warning. 0 OD for earlier models. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. Research-grade results. Molecular Devices' microplate readers. 340 PC384. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Cellular Imaging Systems. The computer controlling the plate reader runs Windows XP. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax:. For customers outside the U. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Select the Driver tab and click Update Driver. The adapter is required for optimum performance with standard 96-well and 384-. Number of Citations*: 8350. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact u@osu. #SpectraMax L Config). The Back Panel Figure 1. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. ~ Bio-Tek ELx808 Microplate Reader $2,795. 20-plate magazine (pair) 0200-6016. 0 . In this product video, see how the SpectraMax M5 quickly reads samples. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Flexibility to read a variety of plate formats. The plate is set up for reading and images are acquired accordingSpectraMax Readers have the most complete level of product validation and compliance. 0 × 103 to 2. 190. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Step 2: Launch SoftMax Pro Software. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Endpoint. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. (Figure 1). #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Incubate at RT. A standard spectrophotometer measures the absorbance of one sample at a time. Expanded wavelength coverageOther readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. Molecular Devices, LLC. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. #0200-7011) Instrument setup SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Company Info. One plate carrier adapter is provided with the instrument. Perform plate-based assays on a large number of plates without having to manually feed them into your microplate washer and reader. 1). Search. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. 76358-624. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and Fig 2: Enzymatic response of synthetic compounds. Learn how Molecular Devices FlexStation 3 Multi-Mode Microplate Reader provides life science industry solutions. Restart the system. Important things to consider when purchasing a multimode microplate reader. Item SpectraMax i3x Multi-Mode Microplate Reader. The absorbance at 412 nm was measured by a SpectraMax i3x Multi-Mode Microplate Reader ( Molecular Devices, San Jose, CA, USA) at 30 °C. Increase productivity with patented microplate reader technologies. (SPR) were monitored by Clone Select Imager (CSI, Molecular Devices, San Jose, CA, USA) and by ELISA assay according to the protocol in Brezinksy etFLIPR Tetra fluorometric imaging plate reader (Molecular Devices, Inc) with EMCCD Camera and 440–480 nm and 515–575 nm emission filters; Methods Cell Preparation. Select Start>Control panel>Device Manager. IMAP® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. S. In addition, the iD5 reader measures TRF and FP and. SpectraTest ABS2 Absorbance Validation Plate SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e,. Molecular Devices' microplate readers. 303 unable to cal dark current. 0 . This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. Retrieving Each. Cellular. Our microplate readers are the industry’s most cited instruments and have empowered life science researchers to advance protein and cell biology, breaking the barriers to novel, landmark discoveries. . Shop a wide selection of Molecular Devices FLIPR TETRA System's on LabX. Detection cartridge modularity allows for easy addition of new read modes within minutes without. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Lower variance: Normalize your plate reader assay data to the number of cells in each well. 4: Test tube cover. #4TI-0214) UltraVision Plate 96 (Brooks Life. Instrument settings can be saved as a protocol file. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. These lab workhorses are used in drug discovery. S. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Plate stacker and robot integration The SpectraMax 340PC384 reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. ORDER. or 0. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Molecular Devices offers a broad range of technologies for high-throughput screening, genomic and cellular analysis, colony selection, and microplate detection. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices. . The plate reader temperature was set to 37°C and allowed to reach 37°C before proceeding. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. absorbance plate readers. 1. Microplate Readers. 997 (n = 3). The plate reader optics are tunable for absorbance,. 50-plate magazine : 0200-6187. Plate chamber design of the SpectraMax category 1 microplate readers. This document is provided to customers who have purchased Molecular Devices, Inc. Endpoint, dual read, kinetic, fast kinetic. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. PURPOSE. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. 5032018C 8 Chapter1:Introduction TheEMax® PlusMicroplateReaderisafilter-basedabsorbancemicroplatereaderwitheight standardfiltersforawiderangeofapplications. Step 1: Capture antibody binds to ELISA plate wells. 00 USD. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. Get a front row view of your cells. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 1. 1. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Built-in near-field communication (NFC) reader enables you to pull up your custom protocols and results with a single tap, saving you precious time. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. 25 to 3. Page 1 of 8 . The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Samples with volumes as low as 2 µL can be measured. #0200-7009) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax i3x reader are shown below. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. Unplug the power cable from the back of the instrument. The reader measures both flat and round 96-well microplates and. Create custom fluorescence assays without having to purchase expensive filter sets. We would like to show you a description here but the site won’t allow us. , call 1-800-635-5577. DOCUMENTATION. Robust, high-value microplate readers that can run samples based on pre-defined. Company Molecular Devices, LLC. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. BioTek Synergy H1 Hybrid OMRF research scientist Kumar Subramani reviewed BioTek Synergy H1, stating it is a configurable multi-mode microplate reader. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. On the back, in the right hand top corner, there. A. 6") Weight. 32 was diluted 10-, 100-,. Feb 22, 2023Used. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. Luminescence was read every five minutes for five hours with three seconds of orbital shaking before each read. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Supersedes 1. 312 gain calibration failed. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. Molecular Devices is one of the leading provider of high-performance bioanalytical measurement solutions for life science research, pharmaceutical and biotherapeutic development. Some familiarity with Windows should be sufficient to use it. 1. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Standard features include a cuvette port,. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. Here, we describe a novel GCaMP6s-CAAX-based calcium assay utilizing a high-throughput fluorometric imaging plate reader (Molecular Devices, Sunnyvale, CA) format that can identify both activators and inhibitors of T-type Ca 2+ channels. Turn on Plate Reader and wait for it to complete its start-up routine. It also integrates with the. APP Automated. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. Instrument settings can be saved as a protocol file. TheUnparalleled performance on a personalized platform. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. If the computer is connected to the Internet: Select Search Automatically for. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Load up the plates that you need to run and walk. 0 × 107) RLU 0. 00. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. Sunnyvale, Calif. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. W×D×H. is user upgradeable—from 2 to 3 modes, providing researchers with a versatile instrument that does not compromise on . 2. This reader's dual monochromators for. A control well was set up to verify the accuracy of the standard curve. Recombinant protein expression Nucleocapsid. 001 EU/mL) was added in order to test the sensitivity of the SpectraMax i3 reader. Supplier: Molecular Devices. 1-1. To contact technical support by phone in the U. Instrument settings can be saved as a protocol file. The SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. The instrument settings were identical to those used in Example 1 above. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. We would like to show you a description here but the site won’t allow us. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. 1) is a fluorescence plate reader that can measure intracellular Ca 2+ concentration using both single- and dual-wavelength fluorescent probes. Data collection speed is sufficient for running fast cell-based kinetic assays. July 17, 2023. Search. Beyond the hardware, a microplate reader’s software also impacts performance and flexibility. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Jun 01, 2004. Plate reader instructions Computer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Page 28 Chapter 5: Read Modes and Read Types 1. The touchscreen interface provides integrated instrument control, data display, and the ability to export results for statistical data analysis. PURPOSE. com 4. View the Molecular Devices website. the iD5 reader’s filter system. M5. integration time per well, depending on firmware version. Reads standard cuvettes & 96-384 well plates from 190 to 1000 nm. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Figure 9. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. 0200-6262. These applications may make use of…FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. Introduction. 635. Cells in each. The system features 96, 384, and 1536 simultaneous liquid transfer, multi-wavelength kinetic reading, fluorescence and luminescence detection, agile internal plate handling, and elevated instrument0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. 340 PC384. The touchscreen interface provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Select the Driver tab. FlexStation 3 reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. coli stock with an OD 600 value of 0. 2. One plate carrier adapter is provided with the instrument. Detector (s) PMT and/or photodiode. Versa Max. 190. Recertification service. READ ANALYZE IMPORTa single plate read using any standard cuvette, or 96- or 384-well microplate. The plate reader optics are tunable for absorbance. Molecular Devices SoftMax ® Pro software was used to analyze and present the data. Restart the system. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. with the kit reagent and transferred to the plate reader for evaluation at the same time. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial. No accessories are needed and SoftMax® Pro Software. A plate reader’s software may offer automated analysis using protocols configured for a particular assay. Explore our fully-integrated,. Retrieving Each. Page 1 of 8 . Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. As Glazer explains, “Our readers have software that analyzes data as it’s acquired from a plate—doing both at the same time. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. 과 같은 Parameter를 검사하여 평가할 수 있습니다. See More Microplate Reader / Microtiter Plate Reader. High-precision 9 channel system; filter-less with up to 6 wavelengths. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. An example of an upgradeable multi-mode plate reader is the SpectraMax M5e, a five-mode reader that is part of the same line as the first microplate reader used in space, thanks to its durability. Find new and used lab equipment and shop a wide selection of products and vendors. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. Visible absorbance -. Sale price $18,499. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of. ®100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. Robust, high value microplate reader designed to deliver research-grade results to any laboratory. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. What plates do you recommend for SpectraMax Plate Readers? Luminescence : Solid white plates for top reads and white plates with clear bottoms for. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. 1. Ratios for each sample were calculated as Read B/Read A. However, when a specific probe is not available, or it causes off-target effects on the. Double-click the unknown device with the yellow warning icon. With optimized reagents, option to operate the. 2. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. It may be named "Pothos". Reader Documentation Version No. Integrate . SpectraMax M5. 13. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Absorbance Plate Reader Comparison Chart. It also allows for the elimination of standard curves. This multi plate reader is excellent to work with for checking the U. Lift up the lid over the chamber to insert or remove a cuvette. Wait 20 seconds. Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. The homogeneous Neurotransmitter Transporter Uptake Assay Kit from Molecular Devices Corporation provides a fast, simple and reliable fluorescence-based. (Molecular Devices cat. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. or washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Please contact your Molecular Devices representative for configuration options. QuickSync. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. The plate reader. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). • 0. Please Inquire. The SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. The test compound menadione was added to cells in a 1:3. 4 °C above ambient to 45 °C. Figure 1.