5°C at 37°C. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Select the lab user profile. 1. The dual monochromator optics allow the. duAl monochromAtors With SpectraMax M2/M2e. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. D 3 1. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization. Allows you to choose your sample format depending on your unique research and throughput needs. SpectraMax M2 and M2e Multi-Mode Microplate Readers User Guide Molecular Devices SpectraMax M2 and M2e Multi-Mode Microplate Readers. BMG LABTECH. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Transcreener ® ADP. , contact. Plate reader settings for NanoOrange assay. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. To schedule a training session please contact Ewa Folta-Stogniew. fluorescence). If something isn't quite right, all three reliable fluorescence and luminescence plate readers can tell you before. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. Whether you need to measure fluorescence, absorbance, luminescence, or time-resolved fluorescence (TRF), or you use PerkinElmer ™ AlphaScreen. We use Spectramax M5 microplate reader in our lab. Microplate readers’ standard detection modes are. Microplate Readers Biotek Synergy 2 Plate Reader Multi-Mode. com 10. 0 out of 5 $ 2,999-20%. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize. The instrument is located in room 2131 at 300 George Street. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. the reader’s stated range. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. 020 OD 2. Luminescence, and Fluorescence microplate re aders—providing extensive data calculation. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Analyze samples using fluorometry, photometry, luminometry, TRF or FP with these accurate, versatile microplate readers. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. The protocol contains settings for reading the plate, as well as template groups that allow a user to easily set up analysis, including calculation. 0) OD (0. The FlexStation 3 reader’s optics are designed to easily adapt to changes in assay requirements while maintaining the optimal performance of a single-mode reader. The purple holder is used for reading non-transparent plates from the top (e. Add to Cart. 0 software was used to handle data analysis and control each instrument. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. 100 readings per well gives optimal Z’ values for this assay. 3 kg. Fluorescence Microplate Reader Comparison Chart. Perform the scan and note the wavelength that gives maximum emission. 100 readings per well gives optimal Z’ values for this assay. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. SpectraMax M2 Readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. SpectraMax Glo Steady-Luc Reporter Assay Kit. Description 1. 3: The cuvette chamber. Features. Auto-calibration and self-diagnostics. duAl monochromAtors With SpectraMax M2/M2e. td. 47 and 0. (a) Chemical structures of 1 (HADP) and its analogues 2–4. Factors that contribute to an instrument’s sensitivity, or limit of detection, include background noise from the detector and electronics, the type of detector that is used, and the instrument’s configuration and. standard spectral absorbance, fluorescence, and luminescence detection. Last lab had ours for years and never had a problem with it. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. SpectraMax® Microplate Readers and SoftMax® Pro Software. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideReader Documentation Version No. Fluorescent Polarization: Solid black plates, as above. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck® Sensor. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western. A solution for low volume applications. spectramax id5, spectramax id3 multi-mode microplate reader, spectramax id3, sof Created Date: 8/20/2020 9:11:23. Fully tested with validations and warranty. Using standard fluorescence optimization can take hours or days to determine the best wavelengths to use for excitation. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (plate reader can measure multiple readouts, including UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. LVF Monochromator™ : Excitation and Emission. 8 foot) 4400-0002 Power cord, 1 meter (3. SpectraTest FL1 Fluorescence Validation . fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). For example, microplates containing different samp les can be read using the same orThe plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. 2. Optimized for fluorescence microplate readers. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. PC software (SkanIt software) Dimensions (D x W x H) 58 x 53 x 51 cm (23 x 21 x 20 in) Weight. Real-time experimental control (REC) that automatically activates a procedure as soon as a specific data value is reached. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. The SpectraMax 190 reader can be integrated with Molecular . In the lower half of the window, left side, there is a box labeled reader. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. The kit includes everything you need: reagents, buffers and a microplate. Figure 6: Transcreener ADP2 FI assay principle. Published Dec 16, 2020 | Updated Dec 16, 2020. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. SpectraMax M2 and use thereof is covered by issued (U. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. 2. . Molecular Devices SpectraMax M2 Multimode Microplate Reader w/ Laptop & Software. UsethecuvetteportforAbsorbance,Fluorescence,andLuminescence(secondary mode)readmodes. Biotek FLx800 Fluorescence Microplate Reader. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Optimized Settings to Validate the Molecular Devices SpectraMax® M2/M2e Microplate Readers with the Transcreener® Fluorescence Intensity Assays. Appendix. Three data types for FP read types are also being introduced with v4. Tristar 3 Multimode Microplate Reader. Fluorescence Microplate Reader Comparison Chart. 4. Molecular devices SpectraMax M5 Pdf User Manuals. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. Molecular Devices SpectraMax M2. Plate chamber design of the SpectraMax category 1 microplate readers. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). BMG Labtech - THERMOstar. We would like to show you a description here but the site won’t allow us. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Item Description FrontPanelOperation SinglewavelengthAbsorbance,%Transmittance,Fluorescence readingofthecuvette(ortesttube) UsingSoftMaxProSoftware ExpressdataasAbsorbance. VANTAstar™ Flexible Multi-mode Microplate Reader. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 3. (b) Fluorescence responses of probe HADP and control compounds. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Specialized microplate readers for luminescence detection are available for use in applications such as luciferase reporter assays, bioluminescence resonance energy transfer (BRET), and other luminescent assays. In the presence of ACE, the PL intensity was increasing by time, while the PL intensity sustained a low and stable fluorescence in 3 hours in the absence of ACE. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). These measurements can include parameters such as absorbance, fluorescence intensity and. Absorbance; Fluorescence (FI, TR, FP) Luminescence. The SpectraMax i3x reader provides theThis Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. The method is based on. Dual monochromators allow selection of any absorbance wavelength between 200 nm and 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The fluorescence was measured in the SpectraMax M3 Microplate reader. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Calcein AM is a widely used live-cell marker. B. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. PC software (SkanIt software) Dimensions (D x W x H) 58 x 53 x 51 cm (23 x 21 x 20 in) Weight. Products : SpectraMax M2/M2e reader, SpectraMax. Fluorescence measurements were acquired at 10 min intervals (ex: 485 nm and em: 535 nm) in a SpectraMax M2 fluorescence plate reader (Molecular Devices) operated in the 384-well microplate format. For fluorescent assays, black plates absorb light and reduce background and crosstalk. The excitation and the emission. The EarlyTox Live/Dead Assay Kit contains two markers for live or dead cells that are suitable for use with mammalian cells. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Analyze the data Step 1: After the microplate has been read, theFluorescence analysis was performed using a SpectraMax iD3 multi-mode plate reader (Molecular Devices LLC, U. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. 2. Compatible automation solutions for SpectraMax Readers. This reader's dual monochromators for. 0 out of 5 $ 9,995 $ 9,495. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 reader measures absorbance, fluorescence, and luminescence. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Results were graphed in SoftMax Pro Software using a 4-parameter curve fit. Get unrivalled sensitivity and reproducibility and elevate your microplate assay protocol with the Eppendorf Assay/Reader Plates. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). SpectraMax ® M2 Multi-Mode Microplate Reader. The SpectraMax Paradigm Detection Cartridge design utilizes an energy intensity optimization technique that allows the user to read up to seven logs of sample concentration in a single pass of a plate without any manual intervention. SpectraMax® M2/M2e Multimode Microplate Readers, with SoftMax® Pro Software, Molecular Devices. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). FI assay. Conclusions. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. Detection Ranges: absorbance 200-1000nm, fluorescence Ex: 250-850nm / fluorescence Em: 360-850nm, luminescence: 360–850 nm. Figure 6: Transcreener ADP2 FI assay principle. 7°C and the bottom to 36. SpectraMax Spectrofluorometers. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Flexibility to read 6- to 384-well plates. 0 out of 5 $ 9,995 $ 9,495. I've got a LT4500 microplate reader and was hoping to digest the pellets in papain overnight, simply mix a measure of that sample with DMMB solution in a 96 well plate and read absorbance at 525nm. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax ® Pro. 1 fmol/well 384-well plate) Luminescence Specifications Wavelength Range 300 to 700 nm Dynamic Range < 6 decades Sensitivity 10 amol ATP typical (flash) Fluorescence Polarization Specifications Light Source. Package performance tests. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. Refurbished. Fig 2: Enzymatic response of synthetic compounds. DNA quantification with fluorescence dyes. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Molecular devices SpectraMax M3 Pdf User Manuals. spr SpectraMax iD5 SpectraTest ABS2 v1. The SPECTRAmax GEMINI XS Microplate Spectrofluorometer from Molecular Devices is a microplate reader capable of detecting signal from either fluorescence or luminescence based assays. Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. The software is easy to use and contains settings for hundreds of commonly used assays, such as BCA and MTT. 58% for the detection module and 2. Its dual light sources, a xenon lamp and LEDs, illuminates samples with incomparable signal strength and superior sensitivity. a multi-detection microplate reader with two-mode cuvette port Spectramax® m2/m2e user guide SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM SpectraMax M2 & SpectraMax…plate and push the “drawer” button (this does not need to be done before opening/exploring the software, but must be done before reading the plate, of course). The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Endpoint, kinetic, spectrum and area-well. . 6 % of reading 4. In the presence of ACE, the PL intensity was increasing by time, while the PL intensity sustained a low and stable fluorescence in 3 hours in the absence of ACE. Fluorescence Microplate Reader Comparison Chart. The percent (4) Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant. 5). (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. 305 reference level saturation. Highly selective for dsDNA over ssDNA or RNA. BioTek Synergy 2. SpectraMax ® M2/M2e: Not Capable: Validated:. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. In addition, the SpectraMax iD5Popular answers (1) If you are going to read the plate from the top, you should use a solid black plate. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Therefore, ADP production is proportional to an increase in fluorescence. Application: For molecular biology testing. Downloads. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. Add to Cart. Figure 2-23: Bottle holder To install the bottle holder: 1. Traditional microplate readers perform mostly biochemical assays—measuring how much light passes through or is emitted by a sample. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The GloMax® line includes high-performance plate readers with a range of detection options. Important things to consider when purchasing a multimode microplate reader. When a. The SpectraMax Plus 384 Microplate Reader for. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). Add to Cart. • Flexible design for future expansion • User upgradable in < 2 minutes • High speed detection for up to 1536-well microplates • Excellent. SpectraMax ABS Plus SpectraTest ABS2 v1. S. The analysis capabilities of SoftMax Pro Software provide quantitation in an easy-to-read, user-customizable report format. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 67. 00. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Select the Driver tab and click Update Driver. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. Add to Cart. White plates reflect the light, enabling even low levels of Luminescence to be detected. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and. 119. Note: Check the Applies to section at the bottom of the page to make this is the proper driver for your plate reader. For example, microplates containing different samp les can be read using the same orThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The pre-configured protocols can be selected for each assay and. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader , SpectraMax M5. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. Plate stacker and robot integration The SpectraMax M2 and M2e readers can be integrated with the A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Fill a clean plate with water. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. 7°C and the bottom to 36. spr * SpectraMax i3(x) SpectraTest ABS2 v1. 0077 OD 0. Temperature variation ± 0. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Gemini XPS Fluorescence Microplate Reader . 60 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time. refurbished. Explore how the Gemini EM Microplate Reader is a flexible spectrofluorometer capable of running many fluorescence-based assays using its dual. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. free tracer decreases, resulting in an overall increase in RFU values. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The drawing shows a standard microplate of 15 mm height covered with a lid. Download PDF. Part Number Description 1-2100-0548 USB computer connection cable, 3 meter (9. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. To avoid these issues and provide the best possible dynamic range between the highest and the lowest measurement values of your assay, fluorescence gain is typically adjusted on the sample with the expected highest signal output (e. 0 out of 5 $ 14,499 $ 13,995-13%. The FI assay was performed using the SpectraMax ® M2 Multi-Mode Microplate Reader. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The plate is set up for reading and images are acquired according to specified parameters. Like all SpectraMax® Readers, the new M3 and M4 readers leverage patented technologies to deliver added value to users. Double-click the unknown device with the yellow warning icon. Molecular Devices Spectra Max Gemini XS fluorescence Microplate reader. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® Assays. 1. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. With exceptional signal-to-noise ratio and a lower detection limit. When a. When a feature or capability applies to only certain readers, this exception is noted. 4. Type: Microplate Reader. Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Tecan’s. For a complete list of the instruments supported by this release of theThe HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. 020 OD 2. D 47 7. S. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. Category 2 and 3 microplate readers use gradient heating as well. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. MPN: 50505. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. 验证检测 SpectraMax 190 的工作状态。 堆板机、机械臂一体化整合 SpectraMax 190 可以在几分钟内整合到 Molecular Devices 的 StakMax 堆板机上。为了实现更高程度的自动化，自动化供应商 合作伙伴已经把我们的酶标仪系统和所有主要合作伙伴的机械臂 进行了整合。I am interested in using a SpectraMax M2 Plate Reader for a spectrophotometric assay and am trying to collect the proper reagents and materials. Danaher Inc spectramax m2 fluorescence microplate reader Spectramax M2 Fluorescence Microplate Reader, supplied by Danaher Inc, used in various techniques. Bottom Monochromator: Fluorescein 2. CA$14,500. Microplate Readers. Category 2 and 3 microplate readers use gradient heating as well. Other technologies such as. Usage/Application: Lab. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. A microplate reader as individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). 5008531B 3 Contents Chapter1:SpectraTestMulti-ModeValidationPlateOverview 4 Multi-ModeProtocols 6 Chapter2:GettingStarted 8 CertificateofCalibration 8SpectraMax M2. The SoftMax Pro Software validation package and IQ/OQ validation protocols include tools for FDA 21 CFR Part 11 compliance. Reads various kinds of 96-wells plates, and is equipped with shaking function. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Molecular Devices SpectraMax M2 Multilabel Microplate Reader. 0 × 107) RLU 0. patent nos. Microplate Readers. It allows you to explore cellular pathways and protein activation and expression in one system. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. 00 CAD. 0 software was used to handle data analysis and control each instrument. Fully tested with validations and warranty. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. Customer Satisfaction is our number one goal All lab equipment in stock already tested and ships in 1-3 days The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. Documentation:Plate readers that are used to detect luminescence, fluorescence and absorbance signals can vary in sensitivity (Figure 2). ₹ 1,80,000 Get Latest Price. Reading Capabilities: cuvette or microplate. Endpoint, kinetic, spectrum. Microplate Readers. Add to Cart. Offer. 3: The cuvette chamber. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Step 5: Click the software’s Read button. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader. E Models. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. 312 gain calibration failed. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. Multimode Reader Comparison Chart. With optimized reagents, option to operate the. Additionally, all BMG LABTECH microplate readers offer the possibility to measure in very small volumes, down to 2 µl of the sample by using the LVis Plate. 1. 5 x 38. The SpectraMax i3 Multi-Mode Microplate Reader and the SpectraMax MiniMax 300 Imaging Cytometer enable both ways of assessing transfection conditions for GFPbased reporter assays. 0 out of 5 $ 7,699. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. Absorbance (monochromator). 5%in96-and384-wellmicroplates. Add to Cart. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Absorbance cuvette port. Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . a multi-detection microplate reader with two-mode cuvette port Spectramax® m2m2e user guide SpectraMax M2e user guide cover 1 1 42106 9:53:28 AM SpectraMax M2 SpectraMax…Spectramax i3 Plate Reader. . Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. After the plate is read, data will appear in the plate. begin reading microplates with seven mouse clicks. The emitted• Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. The SpectraMax® i3 Multi-Mode Detection Platform for absorbance, fluorescence, and luminescence detection, and the ability to add cartridges as new assays are developed. e.